2.1 Flashcards
What is the structure of the Nucleus?
- Double membrane called nuclear envelope which contains pores
- Nucleolus does not have membrane but contains RNA
- Chromatin is genetic material wound around histone proteins. It is stretched out when cell not dividing but when dividing it condenses and coils into chromosomes.
What makes a cell a eukaryote?
- Jelly-like cytoplasm which contains organelles.
- Membrane-bound organelles such as mitochondria, golgi apparatus and endoplasmic reticulum.
- Ribosomes where proteins are assembled.
- Vesicles.
- Cytoskeleton- network of protein filaments (actin & microtubules) which allow organelles to move from one point to another, allow cells (amoeba & lymphocytes) to move, allows muscles to contract.
- Nucleus, surrounded by nuclear envelope, with DNA organised and wound into linear chromosomes.
- Plasma membrane
- Nucleolus which contains RNA; where chromosomes unwind; ribosomes are formed.
Advantage of Optical microscope?
- Relatively cheap
- Easy to use
- Portable (lab or field)
- Can study whole living organisms
What is the magnification of Optical microscope?
1500-2000x
Describe an Optical microscope.
-Uses visible light with a wavelength 400-700nm, meaning objects closer to each other than 200nm will be seen as one object.
Define Magnification.
How many times an image is bigger an image is in comparison to its actual size.
Define Resolution.
How clear an image is, higher the resolution, the greater the detail.
What is the image under a Optical microscope called?
Photo micrograph.
What is the image under a Electron microscope called?
Electron micrograph.
Describe how a Scanning microscope works.
A light beam scans an object point to point and assemble the image on a computer using pixel information.
Advantages of Scanning microscope.
- High resolution
- High contrast.
- Depth selectivity allows both whole organisms and cells to be observed.
Use of Scanning microscope.
Used to examine the fungal filaments in the cornea of the eye in order to diagnose fungal corneal infection.
Describe an Electron microscope.
- Use electrons with wavelength to 0.04nm to create an image.
- Electrons fired from cathode and focused onto specimen to screen/photographic plate using magnets.
Describe a Transmission electron microscope.
- Specimen has to be chemically fixed, -> dehydrated and stained with metal salts.
- Electrons move through specimen and focused onto photographic plate.
- 2D greyscale image produced.
What is the magnification of TEM?
500,000x
Describe SEM.
- Electrons bounce off specimen and focused onto screen.
- Produces 3D greyscale image, false colour added.
- Specimen in vacuum and stained with fine metals.
What is the magnification of SEM?
100,000x
What do electron microscopes have in common?
- Large and expensive
- Require lots of skill
- Specimens in vacuum
- Stained with metal salts can be hazardous to user