2-6: Movement of Bacterial Cells Flashcards

1
Q

What is motility

A

The ability to propel your own movement

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

Give an example of a non-motile bacteria

A

Yersinia pestis, a bacterial pathogen

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

What is the flagellum

A

Large, complex, multi-protein (~50) machine that powers movement

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

Describe the general structure of a flagellum

A

Long, thin filament that acts as propeller, rotated by motor anchored in cell envelope

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

What is swarming

A

Coordinated multicellular movement across a solid surface

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

What are the different flagellar arrangements

A

Peritrichous = many across body/pole
Monotrichous = single at pole
Lophotrichous = many, all at one pole
Amphitrichous = both poles
Atrichous = none

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

“runs” in peritrichous bacteria

A

Counterclockwise, flagella bundle at tail, cell moves forward

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

Descibe flagellar rotation of short “tumbles” in peritrichous bacteria

A

One or more flagella rotate clockwise, therefore bundle falls apart, tumbles, assumes new random orientation

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

What two ways do monotrichous bacteria move

A

Reversible and unidirectional flagella

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

How do monotrichous bacteria with unidirectional flagella move?

A

rotation stops/starts, movement during stops = direction change

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

How do monotrichous bacteria with reversible flagella move

A

Can rotate clockwise or CCW, rotation in the opposite direction will reverse the movement

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

What are the three segments of the flagella

A

1) Filament (long, thin propeller driving movement)
2) Hook (adaptor that connects filament to basal body)
3) Basal body (core of structure, powers rotation, motor is part of)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

What drives rotation of the flagellar motor

A

Proton motive force

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

What are the parts of the gram negative flagellar motor

A

Central rod, MS ring, C ring, P ring, L ring, stator

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

Where are the MS, C, P and L ring located

A

MS = CM
C = cytoplasm
P = peptidoglycan
L = OM

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

What does the stator do

A

couples flow of protons to rotation of MS ring - acts like a turbine

17
Q

What does the MS ring do

A

Rotates rod, ultimately the hook and filament

18
Q

What do the L/P rings do

A

Bearings to help rotation

19
Q

What does the C ring do

A

Generate torque, switch motor direction, flagellin secretion

20
Q

What does gram positive flagellum lack

A

P/L rings

21
Q

What is flagellin

A

Proteins, thousands of which make up the filament

22
Q

How long and wide is the filament

A

5-10um long, 20nm wide

23
Q

Describe the structure of the filament

A

Rigid, helical, and hollow

24
Q

Is the filament highly conserved in bactiera?

A

Yes

25
Q

How is flagellin detected by our immune systems

A

It is an important antigen (H antigen)

26
Q

How is flagellum made

A

Built inside -> out
Produced in cytoplasm, secreted via the hollow filament. New subunits will assemble at the end (outside the cell) with the cap proteins

27
Q

What secretion system is used to export flagellin?

A

Type III secretion system. A related system is used as a protein toxin injection system by certain pathogens

28
Q

Variations in some bacteria flagellar motility?

A

Some use Na+ gradient, spirochetes have axial filament that resides in periplasm resulting in corkscrew motion

29
Q

What is a taxis

A

Directed movement of bacteria using bias random walk

30
Q

what is chemotaxis

A

Movement in direction of gradients (increase or decrease) in concentration of a chemical
which is detected by chemoreceptors

31
Q

How does tumble frequency change with moving TOWARDS a stimulus?

A

Tumbles inhibited, longer runs

32
Q

What is phototaxis?

A

P = movement towards/away from light

33
Q

What is aerotaxis?

A

directed motility in response to O2

34
Q

What is twitching motility

A

Non-flagellar motility, uses a type 4 pilus attaches to surface then retracts like a “grappling hook”