19. Restriction endonuclease 2

Question 1

what is the difference between T4 DNA ligase and E.coli DNA ligase?

Answer 1

T4 DNA ligase can join blunt and sticky ends together

E.col ligase can only join sticky ends together - not blunt!

Question 2

what is an Isoschizomer?

Answer 2

a restriction endonucleases from different sources that recognize the same target sequence.

Question 3

what types of clevage sites can Isoschizomers have?

Answer 3

they can have the same target and cleave site

or they can have the same target and different cleave site

look carefully at the picture