17. Intro to Recombinant DNA Technology Flashcards
1
Q
what is recombinant DNA?
A
DNA from different sourcesis cut and recombined.
They can be genes from any organism
2
Q
List the biochemical processes which have been exploited for recombinant DNA technology
A
cloning -these occur at different levels
- molecular
- celluar
- whole organism
3
Q
Describe a plasmid and show how it can be used in recombinant DNA technology.
A
a plasmid is used for molecular cloning.
transformation of the bacteria to share the plasmid that has the recombinant DNA in it is how molecular cloning works
4
Q
what are the four basic tools used for in molecular cloning?
A
- vectors: carry DNA into hosts (these can be plasmids or phages)
- hosts
- restriction enzymes
- dna ligase
