10. Transcription 1

Question 1

in DNA transcription, what does the coding and anticoding strand of DNA do?

Answer 1

coding - is the non template

anticoding - is the template strand of mRNA

Question 2

what are the three parts of transcription?

Answer 2

1. inititation
2. elongation
3. termination

Question 3

why is RNA more suseseptible to breaking with an added dilute base?

Answer 3

because of the ribose sugar - the hydroxyl group makes it easier to nucleophillic attack

Question 4

what is the secondary structure of RNA? why is it able to do this? and what kind of bonds are these?

Answer 4

• RNA is usually only single stranded, therefore usually only one copy
• there is intra base pairing which makes hair pins and loops in the structure
• these are non watson and crick pairs

Question 5

what are 3 features of RNA polymerase?

(these are three things it cant do)

Answer 5

• no 3’exonucleous activity
• no traditional proofreading (cant go back and check mistakes)
• no primer needed