1.8 factors affecting enzyme action Flashcards
which 2 ways are enzyme- catalysed reactions measured?
- formation of the products
- disappearance of the reactants
for an enzyme to work what must it have?
- an active site
- substrate in which to come in contact with
how do you calculate the rate of reaction using a graph?
- dy/dx
what effect does an increase in temperature have on enzyme action?- to optimum
- increase in kinetic energy
- more collisions so more enzyme- substrate complexes
what effect does an increase in temperature have on enzyme action?- huge increase
- denature enzyme
- change in active site’s shape so less enzyme- substrate complexes
what effect does a decrease in temperature have on enzyme action?
- less kinetic energy
- less collisions so less enzyme- substrate complexes
2 ways in which the pH affects how an enzyme works
- changes the charges on the amino acids so substrate cannot attach to active site so no enzyme substrate complex can form
- causes bonds to breaks so changes the active site’s structure so no enzyme- substrate complex
what effect does an increase in enzyme concentration have on the rate of reaction?
- as the enzyme concentration increases the rate of reaction increases
- until there are more enzymes than there are substrates
- the rate of reaction remains constant from then on
draw the graph of the effect of enzyme concentration on the rate of enzyme action
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what effect does an increase on substrate concentration have on the rate of reaction?
- as the substrate concentration increase the rate of reaction increases
- until there are more substrates than there are enzymes
- rate of reaction remains the same from then on
draw the graph of the effect of substrate concentration on the rate of enzyme activity
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