17 — Physical methods in biology and medicine

Question 1

List the major signals being detected in a flow cytometer. Give the information provided by them.

Answer 1

Question 2

How can a flow cytometric histogram be constructed?

Answer 2

Question 3

What is the dot plot and how can it be constructed?

Answer 3

Question 4

What does gating mean in flow cytometry?

Answer 4

Question 5

How does flow cytometric cell sorting take place?

Answer 5

Question 6

List the advantages of flow cytometry and flow cytometric cell sorting (FACS).

Answer 6

Question 7

What is the operating principle of a confocal laser scanning microscope?

Answer 7

Question 8

What is the principle of atomic force microscopy?

Answer 8

Question 9

What is the resolution limit of atomic force microscopy?

Answer 9

Question 10

Explain the aim and principle of mass spectrometry.

Answer 10

Question 11

List forces and their directions acting on a molecule sedimenting in a centrifuge tube.

Answer 11

Question 12

Define the sedimentation constant and give its unit.

Answer 12

Question 13

How can the density of an unknown macromolecule be determined by sedimentation experiments?

Answer 13

Question 14

How does the sedimentation equilibrium depend on the form factor in the case of sedimentation equilibrium method and why?

Answer 14

Question 15

What is electrophoretic mobility?

Answer 15

Question 16

List the factors influencing the electrophoretic mobility of a macromolecule.

Answer 16

Question 17

What is the principle of isoelectric focusing?

Answer 17