14. Practical biotechnology: embryo manipulations (EMT) and cloning Flashcards
Which out of the following statements is true regarding sex sorted semen preparation?
Higher pregnancy rates are achieved by this method, compared to AI with non-sorted semen.
Approx. 50% of the spermatozoa cells are lost during the preparation of sex sorted semen.
The process does not impair the spermatozoa cells.
Efficacy is 100% in getting the desired male or female offspring.
The device for this process is usually used 24 hours a day for maximal utilization.
The device for this process is usually used 24 hours a day for maximal utilization.
Which out of the following statements is false regarding in vitro embryo production?
Fertilization can be maintained by conventional IVF or by ICSI.
Oocytes of valuable donors can be recovered surgically, while ovaries of slaughtered animals can be used for research purposes.
The oocytes collected from the donors are immature, that is why in vitro maturation is necessary.
Embryos require proper culture media and proper environment during in vitro culture.
In vitro embryo production is a special method for MOET.
In vitro embryo production is a special method for MOET.
Which is the correct order regarding in vitro embryo production?
Oocyte recovery, in vitro culture, in vitro fertilization, in vitro maturation, embryo transfer
Oocyte recovery, in vitro maturation, in vitro fertilization, embryo transfer
Oocyte recovery, in vitro maturation, in vitro fertilization, in vitro culture, embryo transfer
Oocyte recovery, in vitro fertilization, in vitro maturation, in vitro culture, embryo transfer
Oocyte recovery, in vitro fertilization, in vitro culture, embryo transfer
Oocyte recovery, in vitro maturation, in vitro fertilization, in vitro culture, embryo transfer
Which out of the following statements is false regarding transgenesis?
Transgenesis is the process of introducing a transgene into a living organism so that the organism will exhibit a new property and transmit that property to its offspring.
A transgenic animal is an organism which have a new gene originated from another species, and the gene was transmitted by crossing two different specieses.
A transgene is a gene that has been transferred by genetic engineering techniques from one organism/species to another.
A transgenic animal is an organism which have inserted DNA that originated in a different organism/species.
A transgenic animal is an organism which have a new gene originated from another species, and the gene was transmitted by crossing two different specieses.
Which out of the following statements is false regarding chimeras?
Embryonic stem cell injection into blastocysts is a method for producing chimeras.
Chimeras are formed from four (or more) parent cells.
Embryo splitting is a method for producing chimeras.
A chimera is originatied from two (or more) different populations of genetically distinct cells.
Embryo aggregation is a method for producing chimeras.
Embryo splitting is a method for producing chimeras.
Which out of the following gametes or embryos can be efficiently cryopreserved?
Boar semen, bull semen, bovine embryos, sex-sorted bovine embryos.
Bull semen, bovine embryos, sex-sorted bovine embryos, splitted bovine embryos.
Bull semen, bovine embryos, sex-sorted bovine embryos.
Boar semen, bull semen, bovine embryos, sex-sorted bovine embryos, splitted bovine embryos, equine embryos.
Bull semen, bovine embryos, sex-sorted bovine embryos, splitted bovine embryos, equine embryos.
Bull semen, bovine embryos, sex-sorted bovine embryos.
Which out of the following methods cannot be used for increasing the number of female offspring of a valuable cow?
Embryo sexing
Artificial insemination
MOET
Embryo splitting
Artificial insemination
Which out of the following statements is false regarding cloning by somatic cell nuclear transfer?
Reproductive cloning creates a new multicellular organism, genetically identical to the nucleus donor.
Cloning by adult somatic cell nuclear transfer has not been successfull in mammals; though nuclear transfer of embryonic cells has resulted in viable individuals.
Individuals created by cloning are usually fertile.
Cloning by differentiated embroynic cell and adult somatic cell nuclear transfer has already been successfull.
Therapeutic cloning means harvesting „personalized” stem cells that can be used to study human development and to potentially treat diseases.
Cloning by adult somatic cell nuclear transfer has not been successfull in mammals; though nuclear transfer of embryonic cells has resulted in viable individuals.
