13. Practical biotechnology: artificial insemination (AI) and embryo transfer (MOET) Flashcards

1
Q

What kind of methods can be used for the artificial insemination of the bitch?

Intrauterine insemination, surgical insemination through the vaginal fornix.

Transvaginal or intrauterine insemination (both performed by Norwegian catheter), surgical insemination.

Vaginal insemination with Norwegian catheter (fresh semen), surgical insemination (cryopreserved semen).

Conventional, intrauterine, and deep intrauterine insemination.

Transvaginal insemination (fresh semen), intrauterin insemination with Norwegian catheter (cryopreserved semen), surgical insemination.

A

Transvaginal insemination (fresh semen), intrauterin insemination with Norwegian catheter (cryopreserved semen), surgical insemination.

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2
Q

What is the optimal time for performing artificial insemination in the bitch?

At the onset of vaginal bleeding, as this is an obvious sign of oestrus.

Before the onset of progesterone secretion, as elevated level of progesterone is a sign of previous ovulation and pseudopregnancy.

When mainly neutrophil grnaulocytes can be seen in vaginal cytology.

Soon after the LH peak, as this is a good and measurable sign of the ovulation.

13-17 days after the onset of vaginal bleeding, 4-6 days after the LH peak.

A

13-17 days after the onset of vaginal bleeding, 4-6 days after the LH peak.

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3
Q

Which methods can be used for the artificial insemination of the sow?

Conventional insemination, intrauterine insemination, deep intrauterine insemination; each method can be used with fresh semen or cryopreserved semen.

Conventional and intrauterine insemination for fresh semen, deep intrauterine insemination for cryopreserved semen.

Conventional insemination, intrauterine insemination with Norwegian catheter, surgical insemination.

Conventional insemination for fresh semen, intrauterine insemination and deep intrauterine insemination for cryopreserved semen.

Conventional insemination, intrauterine insemination, deep intrauterine insemination; each method is used with fresh semen only.

A

Conventional insemination, intrauterine insemination, deep intrauterine insemination; each method is used with fresh semen only.

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4
Q

Is it possible to efficiently cryopreserve bull and boar semen? Is there a difference between the usage of the semen of the two specieses?

Yes, boar semen is almost allways used after cryopreservation, while bull semen is inseminated freshly.

Yes, bull semen is almost allways used after cryopreservation, while boar semen is inseminated freshly.

No, neither the bull semen, nor the boar semen can be cryopreserved efficiently.

No, both bull semen and boar semen can be cryopreserved efficiently.

A

Yes, bull semen is almost allways used after cryopreservation, while boar semen is inseminated freshly.

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5
Q

Which statement is true for the mare?

Estrus cycle is regular throughout the year with 21 days long cycles, but from November to February the mares are not bred.

The symptoms of being in heat can be recognized easily in a stud containing mares only, as in this case the stallion is not disturbing the mares.

The heat is 3 days long; insemination is to be performed on the 1st and 3rd days.

The symptoms of being in heat can be recognized easily in a stall containing mares only, as in this case the stallion is not disturbing the mares.

Restlessness, teasing each other, lifting the tail to one side, frequent urination, opening and closing the vulva, slight mucous discharge are the symptoms of oestrus.

A

Restlessness, teasing each other, lifting the tail to one side, frequent urination, opening and closing the vulva, slight mucous discharge are the symptoms of oestrus.

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6
Q

What is the goal of MOET?

To achieve higher number of offspring from genetically superior cows.

To utilize partially infertile cows as embryo recipients.

To achieve higher number of embryos by using the semen of genetically superior bulls for the fertilization of superovulated cows.

To utilize the lactation of recipients, following the pregnancy.

To achieve higher number of offspring from genetically superior bulls.

A

To achieve higher number of offspring from genetically superior cows.

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7
Q

What is the optimal time for the artificial insemination of the cow?

Immediately after the onset of standing heat.

12-18 hours after the onset of standing heat.

21 days after the previous insemination.

36-44 hours after the onset of standing heat.

When restlessness, mounting each other, resting chin on another cow can be seen.

A

12-18 hours after the onset of standing heat.

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8
Q

When does the ovulation occur in the sow?

Usually on the 3-5th day of the 5 days long oestrus.

Usually in the onset of the 5 days long oestrus.

Usually in the 36-44th hour of the 60 hours long oestrus.

Usually in the onset of the 36 hours long oestrus.

Usually in the onset of the 60 hours long oestrus.

A

Usually in the 36-44th hour of the 60 hours long oestrus.

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9
Q

Which statement is false regarding MOET?

The goal of superovulating the donor is to provide higher than normal numbers of embryos in the same cycle.

Both the donor and the recipients are valuble, genetically superior animals.

The donor is a valuble, genetically superior animal.

The goal of synchronizing the recipients is to prepare the uterus of the recipient to support implantation.

The synchronization of the recipients is not allways necessary.

A

Both the donor and the recipients are valuble, genetically superior animals.

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10
Q

What kind of method can be used for semen collection from the stallion?

The semen can be collected with the gloved hand method, in the presence of a mare in heat.

The semen can be collected with an artificial vagina filled with air, in the presence of a mare in heat.

The semen can be collected with an artificial vagina, in the presence of a mare in heat.

The semen can be collected with the compression of the bulbus glandis, in the presence of a mare in heat.

The semen can be collected with an artificial vagina, the presence of a mare in heat may hinder semen collection.

A

The semen can be collected with an artificial vagina, in the presence of a mare in heat.

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