1.4 (Lab 2) - Histology Terminology

Question 1

Apical side of epithelial side faces ______

Answer 1

faces the lumen

Question 2

Basal side of epithelial side faces ____

Answer 2

faces the basement membrane

Question 3

adluminal

Answer 3

next to lumen

Question 4

abluminal

Answer 4

away from lumen

Question 5

describe the motion of cilia

Answer 5

they beat in one direction

Question 6

Skeletal muscle fibers are syncytia, define syncytia, and what does it mean in terms of visualizing them on a LM?

Answer 6

• Syncytia = they are formed from several individual cells joining together during development
• Nuclei appear to be pushed to the periphery of the fibers, makes seeing cell membranes of individual skeletal muscles impossible?

Question 7

eosinophilic stains will be what color

Answer 7

pink

Question 8

basophilic stains will be what color

Answer 8

purple

Question 9

what are sterocilia? where can you find them? how does their structure relate to function?

Answer 9

• Sterocilia = long microvilli
• found on epthelial cells lining lumen (we saw in the epididymis slide - buh717)
• long fibers, that tend to clump together and their presence increases the surface area of their cells to aid their function = absorption
• they are nonmotile (unlike, microvilli)

Question 10

What does PAS stain for? What are the typical coloring elements?

Answer 10

• PAS stains for glycoproteins
• glycoprotein rich elements will appear blue
• might allow you to see basement membranes better and apical membranes (but basal is often darker stained)

Question 11

what is the brush border and where is it found

Answer 11

= a microvillous border found on the epithelial surfaces of the small intestine (striated border) and kidney (brush border)

• brush border morphology increases a cell’s surface area
• can be on simple cuboidal epithelium or simple columnar epithelium

Question 12

some cell types of trachea lab

Answer 12

• goblet cell - is not ciliated
• ciliated epithelium
• basal cell - adjacent to basement membrane (Stem cell)
• basement membrane increases in respiratory system with irritant contact over time

Question 13

thymus take home messages from lab

Answer 13

• connective tissue capsule
• lobule (dark whitish outside=cortex; inside lighter=medulla)-as T cells mature they migrate to medulla and are released
• large pink structures in thymus are diagnostic from medulla of thymus

Question 14

take home messages from edpidymus in lab

Answer 14

• not HNE stain
• apical specialization = stereocillia - for absorptive function
• always start at lumen when viewing images

Question 15

take home messages from Kidney in lab

Answer 15

• special stain to recognize the basement membrane
• apical aspect of cells = brush border
• glands have basement membrane (for the most part)