12: Hematopoeisis Definitions Flashcards
Bone marrow hematopoeitic compartment vs marrow stromal compartment
Hematopoeitic: highly vascular with hematopoeitic SCs
Marrow stromal compartment: endothelial cells, adipocytes, stromal cells, fibroblasts, macrophages
Five cell types in BM that produce hematopoeitic GFs
- Endothelium
- Marrow fibroblasts
- Stromal cells
- Adipocytes
- Lymphocytes
Cellularity of bone marrow with age
100-age = cellularity % of bone marrow (ex: I’m 24, so my cellularity should be about 76%)
SCF: Stem Cell Factor: produced by what + function
From fetal tissue + BM -> makes SCs responsive to other cytokines to stimulate hematopoeisis
Key GF in leukemoid reaction
IL-6
GF used in treatment for neutropenia after chemo or BM transplant
G-CSF
What produces EPO vs TPO (erythropoeitin and thrombopoeitin)
EPO: kidney
TPO: liver
What stain is used on reticulocytes to visualize blue reticulum/ribosomes
Supravital stain
When are reticulocytes increased in peripheral blood?
In hemolytic anemias where RBCs are being destroyed
Granulocytes vs agranulocytes
Granulocytes: neutrophils, basophils, eosinophils
Agranulocytes: monocytes, lymphocytes
Agranulopoeisis vs granulopoeisis
Both: cells decrease in size
Agranulopoeisis: heterochromatin increases + no granules form + no nuclear lobulation
Granulopoeisis: chromatin condenses + granules form + nucleus becomes lobulated
What causes indentation of nucleus in neutrophil process?
Para nuclear golgi nestles in to the nucleus
Two substances to note that are secreted by basophils
Histamine, heparin
Nucleus of a plasma cell
Eccentrically placed with perinuclear hoff (Golgi nestled in)
Endomitosis
Nuclear divisions occur without cell division -> polyploid nucleus
