1.1 Flashcards
How does SDS-PAGE separate proteins
By size
How does a centrifuge work
Separate substances of density
What can paper and thin layer chromatography separate?
And 2 egs
Different substances such as amino acids and sugars
Why use Buffers
To control pH
What does the speed of the solute travel along chromatogram depend on
Solubility of Solvent
What is the principal of gel electrophoresis and its use in separating proteins and nucleic acids
Charged macromolecules move through electric field applied to gel matrix
What does native gels separate proteins by
Shape size and charge
What is an isoelectric point
PH at which soluble protein has no net charge and will precipitate out of solution
Why is immunoassay techniques used?
Detect and identify specific proteins
What name is given to the stocks of antibodies with the same specificity
Monoclonal antibodies
What technique is used after SDS-PAGE electrophoresis?
Western blotting
What is western blotting
Separated proteins from gel are transferred to solid medium
How can proteins be identified?
With specific antibodies that have reporter enzymes attached
An antibody specific to the protein antigen is linked to a chemical label. What is the label?
Reporter enzyme producing colour change
What can used instead of reporter enzyme for the label?
Chemiluminescence, fluorescence and other reporters
