Zhou 3-24

Question 1

What is the mechanism to ensure that TFs are routed to the correct location

Answer 1

they are kept in the cytosol by masking the NLS (nuclear localization signal)

Question 2

What are 3 different means of changing the TF localization (“waking up” the TF)

Answer 2

de-posphorylatiing the NLS, degrading the inhibiting factor, homodimerizing, dissociate TF from inhibitor/degrader signal, dissociate from membrane anchor

Question 3

What is the idea for controling the right amount of TF

Answer 3

They are more suceptible to ubiquitination, degradation by becoming activated (Black widow hypothesis). This allows desensitization.

Question 4

TAD

Answer 4

transactivation domain- overlaps with the degron domain

Question 5

How does the cell be sure that the TF has the correct activity

Answer 5

by using a “editor, writer” to open up the chromatin at the modification signal recognized (Docking site)

Question 6

Explain the binary switch hypothesis

Answer 6

Chromatin readers recognize a signal based on two mutually exclusive modifications, where residues are sterically close to one another

Question 7

Why is the histone code so important to genetics

Answer 7

It tell the TFs how when and where genes get expressed