Zhou 3-12

Question 1

3 steps of initiation

Answer 1

1. Forming closed intitation complex with one side of the Pol binding the promoter 2. Forming open (Melted) complex, where ~14bps are separated around the +1 site 3. Promoter escape, where stable RNA, DNA, Enzyme complex forms and elongation begins

Question 2

How many RNA Pol’s does bacteria have? What is the main protein coding RNA Pol in eukaryotes?

Answer 2

bacteria have only one RNA Pol. Eukaryotic RNA Pol. II makes mRNA

Question 3

Prinbow box

Answer 3

TATA box of prokaryotes that is one of 2 consensus seq anchor points for sigma factors of RNA Pol in promoter. located @ -10.

Question 4

Prokaryotic C-terminal Domain factor and its consensus sequence. What is its function?

Answer 4

alpha factor. binds to the UP (Upstream Promoter) element. Works with the sigma initiation factor to recruit RNA Pol to the site. Binds less specifically than sigma.

Question 5

How many components in eukaryotic Polymerase? How many in prokaryotic polymerase?

Answer 5

Both contain 5 different protein components (factors)

Question 6

What is the difference in the pre-initiation complex and the elongation complex in PROKARYOTES

Answer 6

sigma factor is kicked out of the elongation complex with conformation change

Question 7

How do initiation factors differ in prokaryotes versus eukaryotes

Answer 7

Prokaryotes use sigma factors, eukaryotes use general transcription factors

Question 8

alpha- amanitin

Answer 8

mushroom inhibitor of RNAP II eukaryotes

Question 9

How do eukaryotic pre-initiation complexes melt the DNA to form transcription bubble?

Answer 9

Using the ATPase factor TFIIH

Question 10

BRE

Answer 10

TFIIB Recognition Element

Question 11

Inr

Answer 11

Initiatior

Question 12

DPE

Answer 12

Downstream Promoter Element

Question 13

Which factor of eukaryotic RNAP contains the TBP (TATA Binding Protein)

Answer 13

TFIID

Question 14

GTFII’s

Answer 14

General Transcription Factors for RNAPol II: TFIID + TAF’s (TBP Associated Factors)

Question 15

What must occur for eukaryotic RNAPol to escape the promoter

Answer 15

Phosphorylation of the CTD (partially by TFIIH factor, partially by serine kinase elongation factors), shedding on initiation factors/GTF’s, mediators

Question 16

CTD of eukaryotic RNAPol

Answer 16

Many repeats of 7 amino acid peptide- important for mRNA processing and modification

Question 17

TFIIS

Answer 17

elongation factor that performs hydrolytic editing- where more than one NT can be removed- VERY ACCURATE proofreading

Question 18

How many channels in RNAPol? (Both Prokaryotic and eukaryotic)

Answer 18

5 channels