Fried 3-5

Question 1

transition example

Answer 1

A –>G or C–> T

Question 2

transversion example

Answer 2

A–> C or T, T –> A or G, etc.

Question 3

How do mutations arise from mismatches

Answer 3

during replication

Question 4

point mutation

Answer 4

single nucleotide change

Question 5

what is the danger of mutating away from the hydrogen bond- participating regions of the base?

Answer 5

creates different tautomers that change propensity to H-bond in consistant pattern

Question 6

5 proteins involved in mismatch repair

Answer 6

MSH2/MSH6, PCNA, MLH1/PMS2, Exo1, Pol. III, DNA Ligase

Question 7

fxn of MSH2/MSH6 (or MutS, prokaryotes)

Answer 7

recognize deformities caused by mismatch

Question 8

fxn of Exo1

Answer 8

endonuclease: cuts DNA on either side of mutation

Question 9

fxn of PMS2/MLH1 (or MutL in prokaryotes)

Answer 9

Aid in mismatch recognition and loading of Exo1 of mis match repair machinery

Question 10

What are the most common substrates for abasic site creation

Answer 10

Purines- that’s why the reaction is called depurination

Question 11

Most common mutagenic catalyst

Answer 11

water

Question 12

DNA Photolyase

Answer 12

Corrects thymine dimers

Question 13

Most common mismatch

Answer 13

G:T