Zhou 3-14

Question 1

What are the main differences between eukaryotic translation and prokaryotic translation?

Answer 1

prokaryotes may couple the two processes- directly translate the mRNA after it is synthesized, while eukaryotic mRNA must undergo processing to get into the cytoplasm to be translated

Question 2

Rho

Answer 2

6 component RNA binding protein (containing RNA) ring, after transcribing stops, Rho uses ATPase activity to separate ssRNA from elongation complex

Question 3

How can prokaryotes terminate transcription independent of Rho

Answer 3

by using the stem-loop structure (palindromic) of the newly synthesized RNA and weak AU pairing at the end of the transcript while bound to the DNA

Question 4

Which processes are coupled by eukaryotic RNAPol activity?

Answer 4

elongation, termination, and RNA processing

Question 5

3 steps of 5’RNA processing

Answer 5

1) remove alpha Phosphorous of 5’ triphosphate 2) add GMP 3) add methyl group - THe cap gets recognized by a cap-binding protein before translation can occur

Question 6

CPSF

Answer 6

cleavage and polyadenylation specificity factor

Question 7

CstF

Answer 7

cleavage stimulation factor

Question 8

PAP

Answer 8

Poly-A Polymerase