Stamm 4-4 non-coding RNAs Flashcards
sequence of proteins that interact with the immature miRNA (mir)
Drosha, Pasha (DGCR8), Exportin, Dicer, TAR RNA Binding Protein (TRBP), Argonaut
what important modification does Drosha make on mir to enable recognition by exportin
2 nt 3’ overhang
How does the name of the miR change as it is created
nucleus: pri-miRNA, pre-mRNA, cytosol: miRNA
how does miRNA interact with its target
binding non-specifically (hard too predict), but must be complementary in the 5’ seed region of the miRNA
were can one find the PAZ domain and what is its function
PAZ is a domain of argonaute and it holds the 3’ overhang during cleavage
Three outcomes of miRNA binding to a target
prevent translation, direct deadenylation, cleavage/degradation by Argo
difference between miRNA and siRNA
siRNA is 100% complementary to target and usually leads to degradation rather than transcriptional silencing (but still needs a 3’ overhang), needs RDRP
how is abundance of miRNA regulated
post-transcriptional modifications regulate stability