Stamm 4-4 non-coding RNAs Flashcards

1
Q

sequence of proteins that interact with the immature miRNA (mir)

A

Drosha, Pasha (DGCR8), Exportin, Dicer, TAR RNA Binding Protein (TRBP), Argonaut

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2
Q

what important modification does Drosha make on mir to enable recognition by exportin

A

2 nt 3’ overhang

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3
Q

How does the name of the miR change as it is created

A

nucleus: pri-miRNA, pre-mRNA, cytosol: miRNA

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4
Q

how does miRNA interact with its target

A

binding non-specifically (hard too predict), but must be complementary in the 5’ seed region of the miRNA

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5
Q

were can one find the PAZ domain and what is its function

A

PAZ is a domain of argonaute and it holds the 3’ overhang during cleavage

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6
Q

Three outcomes of miRNA binding to a target

A

prevent translation, direct deadenylation, cleavage/degradation by Argo

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7
Q

difference between miRNA and siRNA

A

siRNA is 100% complementary to target and usually leads to degradation rather than transcriptional silencing (but still needs a 3’ overhang), needs RDRP

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8
Q

how is abundance of miRNA regulated

A

post-transcriptional modifications regulate stability

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