Work Practices

Question 1

True or False? Animal aerosolization with toxins can be performed in a Class II BSC.

Answer 1

False

Question 2

When working with toxins that pose a percutaneous hazard, one should:
always wear an appropriate respirator
wear chemical splash goggles
wear gloves that are impervious to the toxin and any diluents/solvents in use
work inside a Class III BSC

Answer 2

Wear gloves that are impervious to the toxin and any diluents/solvents in use

Question 3

Environmental monitoring is useful for which of the following?

Determining the effectiveness of cleaning protocols
Detecting aerosols of organisms in use in lab
Identifying poor technique in use in lab

Answer 3

Determining the effectiveness of cleaning protocols

Question 4

Environmental monitoring often involves the use of which of the following?

Rodac plates
Air sampling
Rinse sampling
All of the above

Answer 4

All of the above

Question 5

Horsfall cage

Answer 5

ventilated containment units and cages with filtered air supply and exhaust for larger animals with BSL2 and 3

Question 6

Aseptic

Answer 6

Absence of pathogenic organisms

Question 7

Sterile

Answer 7

Absence of all living organisms

Question 8

It is recommended you wait to start work for

15-20 minutes after turning on the BSC:

Answer 8

To allow the HEPA filters to electrostatically charge

Question 9

Risk assessment for development of Exposure Control Procedures must be performed and includes the following:

Answer 9

– Agents/potential agents
– Consider routes of transmission
– Availability of treatment, vaccines
– Type and scale of proposed work
– Training and proficiency of workers
– BSL determined locally by biosafety
committee and/or BSO