WK16: Specimen Collection Microbio

Question 1

True or False
Specimen collection in Microbiology to isolate and identify the causative agents forms back bone of the investigative procedures.

Answer 1

True

Question 2

True or False:

Causal testing of Microbiological tests are productive.

Answer 2

False (counterproductive)

Question 3

True or False:

formalin can be used in microbiology specimens

Answer 3

False

Question 4

True or false:

The syringe is held at the shaft during collection

Answer 4

False (butt)

Question 5

Serum is allowed to clot for ____ mins at ambient temp

Answer 5

30

Question 6

Serum is placed at _____ C for at least 1-2 hours during handling

Answer 6

4-8 C

Question 7

Serum is centrifuged at ______ rpm for 5-10 mins

Answer 7

1500 rpm

Question 8

repeated freeze-thaw cycles destroy _____

Answer 8

IgM

Question 9

how many mL of CSF is collected?

Answer 9

3-5mL

Question 10

True or False:
If sudden removal of (CSF) fluid is allowed may draw down
cerebellum into the Foramen magnum and compress the
Medulla of the Brain

Answer 10

True

Question 11

The best site for puncture is inter space between ______ lumbar vertebrae

Answer 11

3 and 4

Question 12

True or False:

if CSF transport is delayed, refrigerate

Answer 12

False (will kill H. influenza)

Question 13

Drug resistant coli form bacilli or yeasts may dominate the ____ _____ in patients
receiving antibiotics.

Answer 13

throat flora

Question 14

A plain ______ should be used to collect as much exudates as possible from tonsils, posterior pharyngeal wall

Answer 14

cotton wool swab

Question 15

If cooperated by patient, the swab should be rubbed with rotation over one tonsillar area of the ______ and uvula, the other tonsillar area and finally the _______

Answer 15

soft palate, posterior pharynx

Question 16

Specimens should be placed into sterile _______ and immediately placed on ice or cold packs or at 4°C

Answer 16

viral transport media (VTM)

Question 17

True or False

All respiratory specimens should be kept at 4°C for no longer than 5 days

Answer 17

false (5 days)

Question 18

True or False:

A deep nasal swab generally yields the same information as throat swab.

Answer 18

True

Question 19

Deep nasal are taken to diagnose ______ and Diphtheria bacillus.

Answer 19

S.pyogenes

Question 20

Pus collected or aspirated from sinus, or a saline wash out should be examined in a Gram film and by culture on _____ and _______blood agar plates.

Answer 20

aerobic

anaerobic

Question 21

as long as eardrum remains intact, none of the
infected exudates can be collected on an ear swab, though culture of the throat swab may give a provisional indication of casual organism

Answer 21

Acute otitis Media

Question 22

Swabs of the discharge in the external meatus should be cultured to guide the choice of antibiotics for systemic and topical therapy.

Answer 22

Chronic suppurative otitis media

Question 23

for otitis externa, A swab should be taken from the _____ and cultured aerobically on blood agar and MacConkey agar plates for the bacteria

Answer 23

meatus

Question 24

minimum volume for sputum

Answer 24

1mL

Question 25

sputum is a mixture of _____ ______ and inflammatory _______ coughed up into the mouth and expectorated

Answer 25

bronchial secretions

exudates

Question 26

Method for collecting urine from neonates

Answer 26

Broom hall method

Question 27

The specimen commonly collected for the diagnosis of vaginitis’s, vaginosis or uterine sepsis is _________

Answer 27

high vaginal swab

Question 28

An ________ must be collected for examination for gonococci.

Answer 28

endocervical swab

Question 29

Swabs for genital infection must be transported in _____ _____ ______

Answer 29

Amie’s transport medium

Question 30

Ideal sample from wounds

Answer 30

pus or exudates

Question 31

Stool must be collected within ____ hours of onset

Answer 31

48

Question 32

Sample amount for stool

Answer 32

5-10 mL

Question 33

Storage temp for stool

Answer 33

-15C

Question 34

For stool sample for suspected parasites, mix with ______ or _______

Answer 34

10% formalin or polyvinyl chloride

Question 35

For parasite stool samples if delay is unavoidable and particularly when the weather is warm collect the specimens in a container holding 6 ml _________ transport medium

Answer 35

buffered glycerol saline