WK16: Specimen Collection Microbio Flashcards
True or False
Specimen collection in Microbiology to isolate and identify the causative agents forms back bone of the investigative procedures.
True
True or False:
Causal testing of Microbiological tests are productive.
False (counterproductive)
True or False:
formalin can be used in microbiology specimens
False
True or false:
The syringe is held at the shaft during collection
False (butt)
Serum is allowed to clot for ____ mins at ambient temp
30
Serum is placed at _____ C for at least 1-2 hours during handling
4-8 C
Serum is centrifuged at ______ rpm for 5-10 mins
1500 rpm
repeated freeze-thaw cycles destroy _____
IgM
how many mL of CSF is collected?
3-5mL
True or False:
If sudden removal of (CSF) fluid is allowed may draw down
cerebellum into the Foramen magnum and compress the
Medulla of the Brain
True
The best site for puncture is inter space between ______ lumbar vertebrae
3 and 4
True or False:
if CSF transport is delayed, refrigerate
False (will kill H. influenza)
Drug resistant coli form bacilli or yeasts may dominate the ____ _____ in patients
receiving antibiotics.
throat flora
A plain ______ should be used to collect as much exudates as possible from tonsils, posterior pharyngeal wall
cotton wool swab
If cooperated by patient, the swab should be rubbed with rotation over one tonsillar area of the ______ and uvula, the other tonsillar area and finally the _______
soft palate, posterior pharynx
Specimens should be placed into sterile _______ and immediately placed on ice or cold packs or at 4°C
viral transport media (VTM)
True or False
All respiratory specimens should be kept at 4°C for no longer than 5 days
false (5 days)
True or False:
A deep nasal swab generally yields the same information as throat swab.
True
Deep nasal are taken to diagnose ______ and Diphtheria bacillus.
S.pyogenes
Pus collected or aspirated from sinus, or a saline wash out should be examined in a Gram film and by culture on _____ and _______blood agar plates.
aerobic
anaerobic
as long as eardrum remains intact, none of the
infected exudates can be collected on an ear swab, though culture of the throat swab may give a provisional indication of casual organism
Acute otitis Media
Swabs of the discharge in the external meatus should be cultured to guide the choice of antibiotics for systemic and topical therapy.
Chronic suppurative otitis media
for otitis externa, A swab should be taken from the _____ and cultured aerobically on blood agar and MacConkey agar plates for the bacteria
meatus
minimum volume for sputum
1mL
sputum is a mixture of _____ ______ and inflammatory _______ coughed up into the mouth and expectorated
bronchial secretions
exudates
Method for collecting urine from neonates
Broom hall method
The specimen commonly collected for the diagnosis of vaginitis’s, vaginosis or uterine sepsis is _________
high vaginal swab
An ________ must be collected for examination for gonococci.
endocervical swab
Swabs for genital infection must be transported in _____ _____ ______
Amie’s transport medium
Ideal sample from wounds
pus or exudates
Stool must be collected within ____ hours of onset
48
Sample amount for stool
5-10 mL
Storage temp for stool
-15C
For stool sample for suspected parasites, mix with ______ or _______
10% formalin or polyvinyl chloride
For parasite stool samples if delay is unavoidable and particularly when the weather is warm collect the specimens in a container holding 6 ml _________ transport medium
buffered glycerol saline
