WK15: Collection, Processing, Preservation of Specimen

Question 1

_____ ______ is an important part of the diagnostic test in the clinical laboratory - it detects the viral and bacterial infection

Answer 1

Serologic testing

Question 2

Substitute in case sink is not available in the area

Answer 2

Hand rub

Question 3

how long will it take for a sample to clot?

Answer 3

1 hr

Question 4

What if during the transferring of the sample to another tube, RBCs are disturbed and serum became reddish?

Answer 4

re-centrifuge

Question 5

if specimen testing is delayed refrigerate at ____ C for more than ____ hrs

Answer 5

-20C, 72 hours

Question 6

Common specimen used

Answer 6

serum

Question 7

what specimen is used for rapid testing?

Answer 7

plasma, whole blood

Question 8

interfere with colorimetric results (Falsely increases results)

Answer 8

Icteric or turbid sample

Question 9

emulsion of fat globules that often appears in serum after eating; during digestion

Answer 9

Chyle

Question 10

What should be done to remove chyle from a sample?

Answer 10

rim and re-centrifuge

Question 11

serum appears dark reddish yellow due to high bilirubin

Answer 11

icteric

Question 12

serum appears milky or turbid

Answer 12

lipemia

Question 13

Complement in the body fluids are inactivated by heating for ___C for ____ mins

Answer 13

56C, 30 mins

Question 14

Refers to the process that destroys the complement activity

Answer 14

Inactivation

Question 15

it interferes with the reactions of certain (syphilis) test and complement component (C1q)

Answer 15

Complement

Question 16

volume carried by a macropipette

Answer 16

> 1000uL

Question 17

volume carried by a micropipette

Answer 17

<1000 uL

Question 18

hollow cylinder which work like a straw and require use of additional suction device (bulb)

Answer 18

Non-piston driven vacuum assisted pipettes

Question 19

used for delivering a particular amount of liquid contained between two calibration marks; used when great accuracy is not require

Answer 19

Graduated

Question 20

marked and calibrated to deliver only one volume of the specified liquid; dispense the exact amount of liquid with a small drop left behind (specified volume)

Answer 20

Volumetric

Question 21

Utilizes a partial vacuum to draw liquid into the tip

Answer 21

Air Displacement Pipette

Question 22

Used in viscous, dense, volatile, radioactive, orvcorrosive samples

Answer 22

Positive Displacement Pipette

Question 23

used for ELISA which uses wells

Answer 23

Multisample pipette

Question 24

difference in the incubation time between the first well pipetted and the last well

Answer 24

Delta time effect

Question 25

More accurate and precise results in using a standard solution

Answer 25

Forward pipetting

Question 26

Used in high viscosity solution, buffer with detergent, and solutions that foam easily

Answer 26

Reverse pipetting

Question 27

reduces the effects of mechanical backlash or slippage of the micrometer gears and assures better accuracy

Answer 27

Dial-down technique

Question 28

Pre-wetting increases the _______ within the tip thus, reducing the variation in the sample evaporation

Answer 28

humidity

Question 29

Aspirating with the pipette perpendicularly improves accuracy up to ___ % ultramicro to ____%

Answer 29

0.5% - 2.5%

Question 30

Immersion depth for 200 -2000 UL

Answer 30

3-6mm

Question 31

True or False:

Maintain tip immersion time of 3-5 seconds after aspiration to prevent lack of aspirate

Answer 31

False 1-2 secs

Question 32

True or False Withdraw tip slowly, smoothly from liquid source to prevent contamination to sample or reagent collected

Answer 32

True