WK15: Collection, Processing, Preservation of Specimen Flashcards
_____ ______ is an important part of the diagnostic test in the clinical laboratory - it detects the viral and bacterial infection
Serologic testing
Substitute in case sink is not available in the area
Hand rub
how long will it take for a sample to clot?
1 hr
What if during the transferring of the sample to another tube, RBCs are disturbed and serum became reddish?
re-centrifuge
if specimen testing is delayed refrigerate at ____ C for more than ____ hrs
-20C, 72 hours
Common specimen used
serum
what specimen is used for rapid testing?
plasma, whole blood
interfere with colorimetric results (Falsely increases results)
Icteric or turbid sample
emulsion of fat globules that often appears in serum after eating; during digestion
Chyle
What should be done to remove chyle from a sample?
rim and re-centrifuge
serum appears dark reddish yellow due to high bilirubin
icteric
serum appears milky or turbid
lipemia
Complement in the body fluids are inactivated by heating for ___C for ____ mins
56C, 30 mins
Refers to the process that destroys the complement activity
Inactivation
it interferes with the reactions of certain (syphilis) test and complement component (C1q)
Complement
volume carried by a macropipette
> 1000uL
volume carried by a micropipette
<1000 uL
hollow cylinder which work like a straw and require use of additional suction device (bulb)
Non-piston driven vacuum assisted pipettes
used for delivering a particular amount of liquid contained between two calibration marks; used when great accuracy is not require
Graduated
marked and calibrated to deliver only one volume of the specified liquid; dispense the exact amount of liquid with a small drop left behind (specified volume)
Volumetric
Utilizes a partial vacuum to draw liquid into the tip
Air Displacement Pipette
Used in viscous, dense, volatile, radioactive, orvcorrosive samples
Positive Displacement Pipette
used for ELISA which uses wells
Multisample pipette
difference in the incubation time between the first well pipetted and the last well
Delta time effect
More accurate and precise results in using a standard solution
Forward pipetting
Used in high viscosity solution, buffer with detergent, and solutions that foam easily
Reverse pipetting
reduces the effects of mechanical backlash or slippage of the micrometer gears and assures better accuracy
Dial-down technique
Pre-wetting increases the _______ within the tip thus, reducing the variation in the sample evaporation
humidity
Aspirating with the pipette perpendicularly improves accuracy up to ___ % ultramicro to ____%
0.5% - 2.5%
Immersion depth for 200 -2000 UL
3-6mm
True or False:
Maintain tip immersion time of 3-5 seconds after aspiration to prevent lack of aspirate
False 1-2 secs
True or False Withdraw tip slowly, smoothly from liquid source to prevent contamination to sample or reagent collected
True
