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IMMUNOSERO > WK13: Compliment Fixation Test > Flashcards

WK13: Compliment Fixation Test Flashcards

1
Q

Person responsible for developing CFTs

A

Jules Bordet

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2
Q

discovered that when cholera bacteria were injected into the peritoneum of a guinea pig immunized against the infection, the pig would rapidly die

A

Richard Pfeiffer

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3
Q

True or False:

Bacteriolysis occurs when bacteria is injected into non-immunized guinea pig

A

False (did not occur - bacteriolysis occurs when the same animal received the antiserum from an immunized animal)

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4
Q

Antiserum loses its power to kill bacteria at ____ C

A

55C

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5
Q

bacteria-killing phenomenon was due to the combined action of two distinct substances: _____ and _____

A

antibody in the antiserum

Alexine (complement)

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6
Q

True or False:
When red cells were added to a normal serum mixed with a specific form of bacteria in a test tube, the bacteria remained active while the red cells were destroyed through the fixation of alexine

A

True

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7
Q

when serum containing the antibody specific to the bacteria was
destroyed, the alexine and the solution separated into a ________

A

layer of clear serum

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8
Q

Determines the presence of syphilis bacteria

A

Wasserman test

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9
Q

CFT is replaced with more direct, sensitive and rapid techniques such as

A

RIAs

EIAs

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10
Q

most common source of fresh complement

A

Guinea pig

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11
Q

complement can be preserved either by ______, _____ or _____

A

lipophilizing
freezing or
by adding
preservatives.

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12
Q

diluent for titration and CFT

A

Physiological saline with added magnesium and calcium ions

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13
Q

Complement fixation test consists of a ______ and ______ both of which can activate complement.

A

test system

indicator system

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14
Q

antibody to sheep red cells which is raised in rabbits

A

Haemolysin (amboceptors)

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15
Q

True or False:
If the serum contains specific, complement activating antibody the complement will be not be activated or fixed by the antigen-antibody complex

A

False (Activated and fixed by antigen-antibody complex)

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16
Q

True or False:

If there is no formation of ag-ab complex, complement will not be fixed

A

True

17
Q

CFT: no hemolysis means that the test is _______

A

POSITIVE for the presence of Abs

18
Q

CFT: when the complement remains, and the red blood cells are lysed in the indicator stage the CFT test is ______

A

NEGATIVE

19
Q

step that detects whether complement has been utilized in the first step or not

A

STEP 2

20
Q

In the 2nd step of CFT, an ________ is added

A

indicator system

21
Q

True or False:
complement is fixed in the first step owing to the presence of antibody there will be no complement left to fix
to the indicator system.

A

True

22
Q

If there is an antibody in the patient’s serum, there will be:
A. No antigen-antibody complex - complement will be present free or unfixed in the mixture
B. no complement left to fix to the indicator system.

A

A. No

antigen-antibody complex - complement will be present free or unfixed in the mixture

23
Q

Advantages of CFT

A

Ability to screen against a large number of viral and bacterial infections at the same time, Economical

24
Q

Disadvantages of CFT

A

• Not sensitive - cannot be used for immunity screening
• Time consuming and labor intensive
• Often non-specific (e.g. cross-reactivity between HSV and
VZV)

25
Q

This modification is used when serums which don’t fix guinea pig complement is to be tested

A

Indirect Complement Fixation Test

26
Q

Horse complement which is non-hemolytic is used

A

Congulatinating Complement Absorption test

27
Q

In Congulatinating Complement Absorption test the indicator system used is sensitized sheep red blood cells
mixed with _______

A

bovine serum

28
Q

Bovine serum contains a beta globulin called ______

A

conglutinin

29
Q

When some bacteria combine with their specific antibody in the presence of complement and some particles such as erythrocytes or platelets

A

Immune Adherence

30
Q

antigen is incubated with patient’s serum in presence of complement. If specific antibody is present, it would immobilize the antigen.

A

Immobilisation test

31
Q

Incubation of a live bacterium with its specific antibody in the presence of complement leads to the lysis of the bacteria cells

A

Cytolytic Test

32
Q

Cytolytic test is the basis of vibriocidal antibody test used to measure _______

A

anti-cholera antibodies

IMMUNOSERO (6 decks)

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