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Application & Methods of Instrumental Analysis in Chemistry > Week 9: High-Performance Liquid Chromatography (HPLC) > Flashcards

Week 9: High-Performance Liquid Chromatography (HPLC) Flashcards

1
Q

What does HPLC stand for?

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2
Q

Explain the basic principle behind HPLC separation.

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3
Q

What are the two essential phases involved in HPLC?

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4
Q

What is the role of the stationary phase in HPLC?

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5
Q

What is the role of the mobile phase in HPLC?

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6
Q

How does the interaction between the stationary and mobile phases affect separation?

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7
Q

What are the main types of HPLC based on the separation mechanism?

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8
Q

Describe normal-phase HPLC and its applications.

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9
Q

Describe reverse-phase HPLC and its applications.

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10
Q

What is ion-exchange chromatography, and how does it work?

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11
Q

Explain size-exclusion chromatography and its applications.

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12
Q

What is affinity chromatography, and where is it used?

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13
Q

What are the main components of an HPLC system?

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14
Q

What is the function of the pump in HPLC?

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15
Q

What are the different types of detectors used in HPLC?

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16
Q

Explain the role of the injector in HPLC.

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17
Q

What is the purpose of the column oven in HPLC?

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18
Q

How does the choice of column affect separation in HPLC?

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19
Q

What factors influence the choice of mobile phase in HPLC?

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20
Q

How does the gradient elution technique work in HPLC?

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21
Q

What is the significance of retention time in HPLC analysis?

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22
Q

How can you optimize resolution in HPLC?

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23
Q

What is the role of method validation in HPLC?

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24
Q

What are common problems encountered in HPLC analysis, and how can they be addressed?

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25
Q

How is HPLC used in pharmaceutical analysis?

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26
Q

How is HPLC used in environmental analysis?

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27
Q

How is HPLC used in food analysis?

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28
Q

How is HPLC used in biomedical research?

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29
Q

How is HPLC used in industrial quality control?

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30
Q

What are the advantages of HPLC compared to other chromatographic techniques?

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Application & Methods of Instrumental Analysis in Chemistry (13 decks)

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