Week 9: High-Performance Liquid Chromatography (HPLC) Flashcards
What does HPLC stand for?
Explain the basic principle behind HPLC separation.
What are the two essential phases involved in HPLC?
What is the role of the stationary phase in HPLC?
What is the role of the mobile phase in HPLC?
How does the interaction between the stationary and mobile phases affect separation?
What are the main types of HPLC based on the separation mechanism?
Describe normal-phase HPLC and its applications.
Describe reverse-phase HPLC and its applications.
What is ion-exchange chromatography, and how does it work?
Explain size-exclusion chromatography and its applications.
What is affinity chromatography, and where is it used?
What are the main components of an HPLC system?
What is the function of the pump in HPLC?
What are the different types of detectors used in HPLC?
Explain the role of the injector in HPLC.
What is the purpose of the column oven in HPLC?
How does the choice of column affect separation in HPLC?
What factors influence the choice of mobile phase in HPLC?
How does the gradient elution technique work in HPLC?
What is the significance of retention time in HPLC analysis?
How can you optimize resolution in HPLC?
What is the role of method validation in HPLC?
What are common problems encountered in HPLC analysis, and how can they be addressed?
