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Application & Methods of Instrumental Analysis in Chemistry > Week 3: UV, FIA, and Fluoresces Spectroscopy > Flashcards

Week 3: UV, FIA, and Fluoresces Spectroscopy Flashcards

1
Q

What is the range of wavelengths for ultraviolet (UV) light?

A

The range of wavelengths for ultraviolet light is approximately 10 nm to 400 nm.

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2
Q

True or False: Flow Injection Analysis (FIA) is a technique used for the automatic analysis of samples.

A

True

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3
Q

Fill in the blank: The primary purpose of Flow Injection Analysis is to ________ samples for chemical analysis.

A

inject

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4
Q

What type of spectroscopy utilizes the fluorescence of a substance to analyze its properties?

A

Fluorescence spectroscopy

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5
Q

What is the principle behind fluorescence spectroscopy?

A

Fluorescence spectroscopy is based on the emission of light by a substance that has absorbed light or other electromagnetic radiation.

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6
Q

Which of the following is NOT a component of a typical Flow Injection Analysis system? A) Sample injector B) Detector C) Calibration curve D) Pump

A

C) Calibration curve

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7
Q

What is the main advantage of using Flow Injection Analysis?

A

The main advantage of Flow Injection Analysis is its ability to analyze multiple samples quickly and efficiently.

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8
Q

True or False: Ultraviolet light can cause chemical reactions in certain substances.

A

True

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9
Q

What is the common detector used in fluorescence spectroscopy?

A

Photomultiplier tube (PMT)

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10
Q

Fill in the blank: In fluorescence spectroscopy, the ________ is the light source that excites the sample.

A

excitation source

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11
Q

What is the typical use of ultraviolet spectroscopy?

A

Ultraviolet spectroscopy is typically used to identify and quantify substances based on their absorption of UV light.

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12
Q

What is the Beer-Lambert Law?

A

The Beer-Lambert Law relates the absorption of light to the properties of the material through which the light is traveling.

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13
Q

True or False: Flow Injection Analysis requires manual sample preparation for each analysis.

A

False

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14
Q

What does the term ‘quenching’ refer to in fluorescence spectroscopy?

A

Quenching refers to the decrease in fluorescence intensity due to various factors, such as concentration or environmental changes.

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15
Q

What is the main difference between UV absorbance and fluorescence spectroscopy?

A

UV absorbance measures the amount of light absorbed by a sample, while fluorescence spectroscopy measures the light emitted by the sample after excitation.

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16
Q

Fill in the blank: The ________ is a common application of Flow Injection Analysis.

A

monitoring of chemical processes

17
Q

What type of molecules typically fluoresce?

A

Fluorescent molecules are often organic compounds with conjugated double bonds.

18
Q

What is one limitation of fluorescence spectroscopy?

A

One limitation of fluorescence spectroscopy is that not all substances fluoresce.

19
Q

True or False: Flow Injection Analysis can be used for both qualitative and quantitative analysis.

A

True

20
Q

What are the two main components of a fluorescence spectrometer?

A

The two main components are the excitation source and the emission detector.

21
Q

What is the role of a solvent in UV spectroscopy?

A

The solvent serves as a medium for the sample and can affect the absorption characteristics.

22
Q

Fill in the blank: In fluorescence spectroscopy, the ________ is the wavelength of light emitted by the sample.

A

emission wavelength

23
Q

What are typical applications of ultraviolet spectroscopy?

A

Typical applications include analyzing proteins, nucleic acids, and various organic compounds.

24
Q

What is the purpose of a calibration curve in analytical chemistry?

A

A calibration curve is used to determine the concentration of an unknown sample based on its measured response.

25
Q

True or False: Flow Injection Analysis can be integrated with other analytical techniques.

A

True

26
Q

What is the significance of the Stokes shift in fluorescence spectroscopy?

A

The Stokes shift is the difference between the excitation and emission wavelengths, indicating energy loss during the process.

27
Q

What is the effect of pH on fluorescence measurements?

A

pH can affect the ionization state of fluorescent molecules, thereby influencing their fluorescence properties.

28
Q

Fill in the blank: The ________ of a sample is determined by its ability to absorb UV light.

A

concentration

29
Q

What is a common source of UV light in spectroscopy?

A

A common source of UV light is a deuterium lamp.

30
Q

What are the advantages of using fluorescence over UV absorbance?

A

Fluorescence offers higher sensitivity and specificity compared to UV absorbance.

Application & Methods of Instrumental Analysis in Chemistry (13 decks)

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