Week 3: UV, FIA, and Fluoresces Spectroscopy Flashcards
What is the range of wavelengths for ultraviolet (UV) light?
The range of wavelengths for ultraviolet light is approximately 10 nm to 400 nm.
True or False: Flow Injection Analysis (FIA) is a technique used for the automatic analysis of samples.
True
Fill in the blank: The primary purpose of Flow Injection Analysis is to ________ samples for chemical analysis.
inject
What type of spectroscopy utilizes the fluorescence of a substance to analyze its properties?
Fluorescence spectroscopy
What is the principle behind fluorescence spectroscopy?
Fluorescence spectroscopy is based on the emission of light by a substance that has absorbed light or other electromagnetic radiation.
Which of the following is NOT a component of a typical Flow Injection Analysis system? A) Sample injector B) Detector C) Calibration curve D) Pump
C) Calibration curve
What is the main advantage of using Flow Injection Analysis?
The main advantage of Flow Injection Analysis is its ability to analyze multiple samples quickly and efficiently.
True or False: Ultraviolet light can cause chemical reactions in certain substances.
True
What is the common detector used in fluorescence spectroscopy?
Photomultiplier tube (PMT)
Fill in the blank: In fluorescence spectroscopy, the ________ is the light source that excites the sample.
excitation source
What is the typical use of ultraviolet spectroscopy?
Ultraviolet spectroscopy is typically used to identify and quantify substances based on their absorption of UV light.
What is the Beer-Lambert Law?
The Beer-Lambert Law relates the absorption of light to the properties of the material through which the light is traveling.
True or False: Flow Injection Analysis requires manual sample preparation for each analysis.
False
What does the term ‘quenching’ refer to in fluorescence spectroscopy?
Quenching refers to the decrease in fluorescence intensity due to various factors, such as concentration or environmental changes.
What is the main difference between UV absorbance and fluorescence spectroscopy?
UV absorbance measures the amount of light absorbed by a sample, while fluorescence spectroscopy measures the light emitted by the sample after excitation.
Fill in the blank: The ________ is a common application of Flow Injection Analysis.
monitoring of chemical processes
What type of molecules typically fluoresce?
Fluorescent molecules are often organic compounds with conjugated double bonds.
What is one limitation of fluorescence spectroscopy?
One limitation of fluorescence spectroscopy is that not all substances fluoresce.
True or False: Flow Injection Analysis can be used for both qualitative and quantitative analysis.
True
What are the two main components of a fluorescence spectrometer?
The two main components are the excitation source and the emission detector.
What is the role of a solvent in UV spectroscopy?
The solvent serves as a medium for the sample and can affect the absorption characteristics.
Fill in the blank: In fluorescence spectroscopy, the ________ is the wavelength of light emitted by the sample.
emission wavelength
What are typical applications of ultraviolet spectroscopy?
Typical applications include analyzing proteins, nucleic acids, and various organic compounds.
What is the purpose of a calibration curve in analytical chemistry?
A calibration curve is used to determine the concentration of an unknown sample based on its measured response.
True or False: Flow Injection Analysis can be integrated with other analytical techniques.
True
What is the significance of the Stokes shift in fluorescence spectroscopy?
The Stokes shift is the difference between the excitation and emission wavelengths, indicating energy loss during the process.
What is the effect of pH on fluorescence measurements?
pH can affect the ionization state of fluorescent molecules, thereby influencing their fluorescence properties.
Fill in the blank: The ________ of a sample is determined by its ability to absorb UV light.
concentration
What is a common source of UV light in spectroscopy?
A common source of UV light is a deuterium lamp.
What are the advantages of using fluorescence over UV absorbance?
Fluorescence offers higher sensitivity and specificity compared to UV absorbance.
