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HEMA > Week 8 Lab: Smearing, Reticulocyte Count > Flashcards

Week 8 Lab: Smearing, Reticulocyte Count Flashcards

1
Q

Blood smear preparations allow the technician to perform a?

A

differential white blood cell count and morphological study

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2
Q

Blood used for smearing?

A

EDTA anticoagulated blood

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3
Q

Types of smears?

A
  • Cover glass smears
  • Wedge smears
  • Spun smears
  • Buffy coat smears
  • Thick blood smears
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4
Q

Use of cover glass smears?

A

more even distribution of white cells than wedge smear

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5
Q

Use of buffy coat smears?

A

for differential counting of patients with <1.0x10^9/L WBC count

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6
Q

Use of thick blood smears?

A

blood parasites

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7
Q

Steps in performing a Wedge smear?

A
  1. Obtain a clean glass slide, a spreader slide, EDTA blood, and a plain microhematocrit tube
  2. Fill the microhematocrit tube and carefully place a small drop of blood (10 uL) in the middle of the slide, approximately 1 cm from the labeled end (if using blood from finger or heel, careful not to touch the skin with the slide)
  3. Place the slide on a flat tabletop
  4. With the thumb and index finger of the left hand, hold the two edges of the slide.
  5. With the right hand, hold the spreader slide with the thumb on the edge of one side and the other four fingers on the edge of the other side
  6. Place the end of the spreader slide slightly in front of the drop of blood
  7. There should be an approximate 30-40 degrees angle between the two slides
  8. Draw the spreader slide back toward the drop of blood. The blood will begin to spread to the edge of the spreader slide.
  9. Keeping the angle, push the spreader slide rapidly over the entire length of the slide
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8
Q

How to produce a thinner wedge smear?

A

P - pressure increased
A - angle decreased
S - size of blood drop decreased
S - speed of spread decreased

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9
Q

How to produce a thicker wedge smear?

A

P - pressure decreased
A - angle increased
S - size of blood drop increased
S - speed of spread increased

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10
Q

After the ____________ loses its nucleus, a small amount of RNA remains in the red blood cell and the cell is known as ____________.

A

orthochromatic normoblast; reticulocytes

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11
Q

To detect the presence of RNA, the red blood cells must be stained while they are living, and this process is called?

A

supravital staining

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12
Q

In reticulocyte count, the number of reticulocytes in _________ is determined.

A

1,000 red blood cells

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13
Q

These are young RBCs released from the bone marrow.

A

Reticulocytes

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14
Q

True or false: Reticulocytes are not normal in the peripheral circulation.

A

False

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15
Q

True or false: High reticulocyte count in the blood is normal.

A

False

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16
Q

Clinical significance of reticulocyte count?

A

If the physician wants to determine the response to anemia.

17
Q

Materials used for reticulocyte count?

A
  • Slides
  • Anticoagulated blood
  • Supravital stain
  • Pipette
  • Microscope
  • Cedarwood oil
18
Q

Supravital stains used for reticulocyte count?

A
  1. Brilliant cresyl blue
  2. New methylene blue
19
Q

Procedure for Reticulocyte count?

A
  1. Place three drops of reticulocyte stain in a small test tube.
  2. Add three drops of well-mixed whole blood to the tube containing the stain.
    nung experiment, gumamit tayo ng 5 drops of stain and 5 drops of blood
  3. Mix the tube and allow it to stand at room temperature or incubate at 37C for 15 minutes
    inincubate natin using a dry bath
  4. After 15 minutes, mix the contents of the tube well and prepare smears and allow it to dry.
    dahil malabnaw ang mixture, bilisan ang pag-smear para manipis ang smear na maproproduce. mabilis lang matuyo ang smear, maximum of 5 mins
  5. Examine the smear under the oil immersion objective.
  6. Count the total number of reticulocytes found per 1,000 RBCs.
20
Q

Which area of the smear will you count the reticulocytes?

A

near the feathered edge, wherein cells are barely touching each other

21
Q

How many oil immersion fields are required for reticulocyte count?

A

10 consecutive OIF = 1,000 RBCs

22
Q

How will you count 10 OIF?

A

moving the stage downwards 10 times

23
Q

Formula for reticulocyte count?

A

Reticulocyte Count (%) = (Number of Reticulocytes Counted / 1,000) x 100

24
Q

Reference range of reticulocyte count for adults?

A

Adult: 0.5-1.5%

25
Q

Reference range of reticulocyte count for newborn?

A

Newborn: 2.0-6.0%

HEMA (13 decks)

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