Week 6- Molecular Biology Techniques

Question 1

DNA:

1) techniques
2) clinical applications
3) emerging application

Answer 1

1) restriction enzyme digestion, Southern blotting, conventional sequencing, PCR
2) Identification of mutations, diagnosis of infectious agents
3) prenatal screening

Question 2

mRNA

1) techniques
2) clinical applications
3) emerging application

Answer 2

1) Northern blotting, RT-PCR, microarray analysis,
2) Breast cancer stratification
3) occular melanoma, breast cancer subtypes

Question 3

protein

1) techniques
2) clinical applications
3) emerging application

Answer 3

1) ELISA, Western blotting
2) diagnosis of infectious diseases
3) therapeutic antibodies

Question 4

somatic mutations

Answer 4

only in one cell- won’t be passed on to future generations

Question 5

Restriction enzyme digestion

Answer 5

• Bind to certain DNA sequences and cleave it to be identified
• Fragments can be put into gel electrophoresis
• Smaller fragments migrate farther than larger fragments
• Pattern of fragments is identifying characteristic of a piece of DNA