Week 5 Flashcards

1
Q

5 Carbon sugar, Phosphate group and Nitrogenous base, make up what?

A

Nucleotides

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2
Q

Phosphate group is always attached to what sugar molecule

A

Number 5

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3
Q

How do nucleotides bond together?

A

Bond between phosphate group and carbon 3 of adjacent nucleotide

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4
Q

Nucleotides are read how?

A

From 5’ end to 3’ end (G-A-T-C)

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5
Q

DNA rings are made of?

A

Bases pointing inwards

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6
Q

Sides of DNA are made of?

A

Sugar-phosphate group

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7
Q

T/F DNA runs anti-parallel (3’-5’)(5’-3’)

A

True

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8
Q

A/T paired by how many bonds?

A

Two hydrogen bonds

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9
Q

C/G paired by how many bonds?

A

Three hydrogen bonds

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10
Q

Which cell has circular chromosome shape?

A

Prokaryotic

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11
Q

DNA replication forms what?

A

Two identical DNA molecules

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12
Q

DNA replication begins with?

A

Origin (A-T rich area w weaker bonds separates)

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13
Q

What enzyme binds at origin and initiated replication?

A

DNA Polymerase

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14
Q

Nucleotides are added where?

A

At replication forks

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15
Q

Gene Expression is the same as?

A

protein synthesis

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16
Q

What is gene expression?

A

conversion of gene specific DNA sequence into functional protien

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17
Q

T/F Genes are expressed on a as needed basis?

A

True

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18
Q

Transcription and translation are part of?

A

Gene expression

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19
Q

Transcription is…

A

DNA sequence –> mRNA sequnce

20
Q

Translation is…

A

mRNA –> Protien

21
Q

Transcription is mediated by what enzyme?

A

RNA Polymerase

22
Q

RNA polymerase attaches where?

A

Promoter

23
Q

T/F synthesis of complementary mRNA is done by RNA polymerase?

A

True

24
Q

Where is mRNA synthesis ended?

A

Termination site

25
Q

T/F both DNA strands are transcribed?

A

False, only one is- called non-coding strand (AKA: template strand)

26
Q

Transcription factors bind the promoter and RNA polymerase recognize these proteins in what kind of cells?

A

Eukaryotic

27
Q

Eukaryotic genes are interrupted by?

A

Intorns/Extrons (non-coding sections)

28
Q

non-coding sequence of DNA in gene

A

Introns

29
Q

Types of RNA

A

mRNA, tRNA, rRNA

30
Q

Translation ribosomes read mRNA in?

A

3 nucleotide sections called codons

31
Q

Codons specify?

A

a specific amino acid that is joined together in sequence

32
Q

All amino acids start with?

A

methionine (met, M)

33
Q

UAA, UAG, UGA are?

A

Stop codons, terminate translation

34
Q

Mechanism enhancing genetic variability?

A

Horizonal gene transfer

35
Q

bacteria enhance genetic variability by?

A

Transformation, Transduction, Conjugation, Transposons

36
Q

Transformation is?

A

bacteria take DNA from enviro (Dead bacteria release DNA into enviro)

37
Q

Transduction is?

A

Bacteriophages transfer DNA from one bacteria to another.

38
Q

Donor bacterium transfers segments of DNA into another bacterium via sex pilus.

A

Conjugation

39
Q

Jumping genes that relocate other parts of genome

A

Transposons

40
Q

Mutations occur because of?

A

Spontaneous, chemical, free radicals, radiation

41
Q

Mutations are usually stopped by?

A

Cellular repair mechanisms

42
Q

Neutral mutation

A

same amino acid is substituted

43
Q

missense mutation

A

different amino acid is substituted due to mutation

44
Q

nonsense mutation

A

early termination of translation due to stop codon mutation

45
Q

Frameshift mutation

A

Base is added or deleted mistakenly shifting frame of mRNA