Week 4 Flashcards
Measurement of light scattered at an angle used to quantitate antigen-antibody complexes best describes:
nephelometry
The expression of cell surface markers is determined in the flow cytometer by what?
fluorescence
In most flow cytometers, labeled cells:
scatter the light and emit fluorescence
As measured in flow cytometry, cells that are the smallest and have the least granules would be identified as what type of cell?
lymphocytes
What is an intrinsic parameter that can be measured by a flow cytometer?
internal complexity
What is meant by “gating” in flow cytometry?
an electronic window separating subpopulations of cells
Name the steps are included in a Southern blot.
gel electrophoresis followed by transfer of the pattern to a membrane
A short strand of DNA or RNA used to detect an unknown nucleic acid in a patient specimen best describes what?
nucleic acid probe
A reaction characterized by amplification of a specific region of a DNA strand through denaturing and annealing best describes what?
polymerase chain reaction
In order for a probe to hybridize to the target, what condition must be met?
The target must have complementary bases.
In flow cytometry, if there are two light detectors, what does the amount of side scatter indicate?
granularity of the cell
What type of assay is being performed when patient serum + labeled Ag + known Ab are added, incubated, and then washed free of unbound material?
competitive EIA
In an ELISA assay, the amount of enzyme activity and the amount of patient analyte are in what proportions?
Directly proportional
How abundant are binding sites in competitive binding assays?
A limited number of binding sites that are present
An immunoassay described as more sensitive to agglutination reactions involving a concentration that is directly proportional to the amount of color, and requires washing carefully very important.
Noncompetitive enzyme immunoassay
