Week 3

Question 1

The binding of one Fab on an immunoglobin to one epitope on an antigen is

Answer 1

affinity

Question 2

Prozone is

Answer 2

A situation in which antibody is in excess

Question 3

What type of an assay describes an antigen an antibody combining to form an insoluble complex that is detected macroscopically?

Answer 3

precipitation

Question 4

Non-identity reactions are described as?

Answer 4

The formation of precipitation in an X shape between two adjacent antigen wells.

Question 5

Equivalence zone is described as

Answer 5

The zone at which antibody and antigen are at optimal concentrations to form a large insoluble lattice together.

Question 6

The diameter of the precipitin ring correlates with what?

Answer 6

The concentration of antigen in radial immunodiffusion

Question 7

How are the strength of different types of attractions between an individual antigen and antibody molecule described?

Answer 7

avidity

Question 8

Precipitation is involved in what?

Answer 8

The radial immunodiffusion test

Question 9

Ouchterlony is an example of?

Answer 9

A qualitative precipitation procedure

Question 10

Postzone is?

Answer 10

A situation in which antigen is in excess as compared to antibody

Question 11

A continuous arc of precipitate is seen between two adjacent wells in an Ouchterlony immunodiffusion procedure, what type of a reaction is described?

Answer 11

identity

Question 12

Rocket immunoelectrophoresis is?

Answer 12

Radial immunodiffusion combined with electrophoresis

Question 13

The zone of equivalence is?

Answer 13

Where lattice formation occurs at the greatest extent

Question 14

The initial forced of attraction between antigen and antibody is?

Answer 14

Affinity

Question 15

Decreasing the strength of the current, changing pH of buffer, and increasing the strength of the current, all adversely affect what?

Answer 15

electrophoresis reaction

Question 16

What technique can be used to best determine if two fungal antigens are identical?

Answer 16

Ouchterlony immunodiffusion

Question 17

Immunofixation is an example of

Answer 17

A Western blot test used to confirm HIV

Question 18

RID is what type of a diffusion reaction?

Answer 18

Single diffusion reaction

Question 19

Soluble antigens and Soluble antibodies are involved in what reaction?

Answer 19

Precipitation reactions

Question 20

Agglunation is

Answer 20

Serological reaction in which the antigen is bound to a large carrier, the soluble antibody is bound and formed into an insoluble complex that can be detected macroscopically.

Question 21

Hemagglutination is?

Answer 21

Serological test that uses red blood cells coated with exogenous antigens (like bacterial polysaccharides) in order to detect patient antibodies in said exogenous antigen.

Question 22

Passive agglunation is described as

Answer 22

When carrier particles are coated with antigen that is not normally found on them

Question 23

Antibodies that have been coated patient red blood cells in vivo are found positive on which test?

Answer 23

Coombs’ Test

Question 24

Coombs reagent is used for?

Answer 24

Enhancing agglutination with IgG coated red blood cells

Question 25

IgG is sometimes used to?

Answer 25

To visually enhance the reaction.