Week 3 Flashcards
The binding of one Fab on an immunoglobin to one epitope on an antigen is
affinity
Prozone is
A situation in which antibody is in excess
What type of an assay describes an antigen an antibody combining to form an insoluble complex that is detected macroscopically?
precipitation
Non-identity reactions are described as?
The formation of precipitation in an X shape between two adjacent antigen wells.
Equivalence zone is described as
The zone at which antibody and antigen are at optimal concentrations to form a large insoluble lattice together.
The diameter of the precipitin ring correlates with what?
The concentration of antigen in radial immunodiffusion
How are the strength of different types of attractions between an individual antigen and antibody molecule described?
avidity
Precipitation is involved in what?
The radial immunodiffusion test
Ouchterlony is an example of?
A qualitative precipitation procedure
Postzone is?
A situation in which antigen is in excess as compared to antibody
A continuous arc of precipitate is seen between two adjacent wells in an Ouchterlony immunodiffusion procedure, what type of a reaction is described?
identity
Rocket immunoelectrophoresis is?
Radial immunodiffusion combined with electrophoresis
The zone of equivalence is?
Where lattice formation occurs at the greatest extent
The initial forced of attraction between antigen and antibody is?
Affinity
Decreasing the strength of the current, changing pH of buffer, and increasing the strength of the current, all adversely affect what?
electrophoresis reaction
What technique can be used to best determine if two fungal antigens are identical?
Ouchterlony immunodiffusion
Immunofixation is an example of
A Western blot test used to confirm HIV
RID is what type of a diffusion reaction?
Single diffusion reaction
Soluble antigens and Soluble antibodies are involved in what reaction?
Precipitation reactions
Agglunation is
Serological reaction in which the antigen is bound to a large carrier, the soluble antibody is bound and formed into an insoluble complex that can be detected macroscopically.
Hemagglutination is?
Serological test that uses red blood cells coated with exogenous antigens (like bacterial polysaccharides) in order to detect patient antibodies in said exogenous antigen.
Passive agglunation is described as
When carrier particles are coated with antigen that is not normally found on them
Antibodies that have been coated patient red blood cells in vivo are found positive on which test?
Coombs’ Test
Coombs reagent is used for?
Enhancing agglutination with IgG coated red blood cells
IgG is sometimes used to?
To visually enhance the reaction.
