Week 4 Flashcards
1
Q
What are the acid-fast stain reagents?
(HINT: BAM)
A
- Basic Fuschin
- Acidified Alcohol
- Methelyne Blue
2
Q
What is Mycolic Acid?
A
A barrier, protecting the cells from dehydrating, as well as from phagocytosis by immune system cells in a host
3
Q
When would you use the acid-fast stain? What Example could we use for Acid fast stain?
A
to test for TB (Tuberculosis), M. Gordonae
4
Q
Steps for Acid-Fast Stain
A
- Mycobacteria is heat fixed as a smear
- Add primary stain Basic Fushin while the slide is heated over the heat bath
- Decolorize with Acid Alcohol
5
Q
What color would acid-fast stains be?
A
red