Week 4 Flashcards

1
Q

What are the acid-fast stain reagents?
(HINT: BAM)

A
  1. Basic Fuschin
  2. Acidified Alcohol
  3. Methelyne Blue
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2
Q

What is Mycolic Acid?

A

A barrier, protecting the cells from dehydrating, as well as from phagocytosis by immune system cells in a host

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3
Q

When would you use the acid-fast stain? What Example could we use for Acid fast stain?

A

to test for TB (Tuberculosis), M. Gordonae

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4
Q

Steps for Acid-Fast Stain

A
  1. Mycobacteria is heat fixed as a smear
  2. Add primary stain Basic Fushin while the slide is heated over the heat bath
  3. Decolorize with Acid Alcohol
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5
Q

What color would acid-fast stains be?

A

red

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