Week 1

Question 1

What is gram staining?

Answer 1

A method used to differentiate bacterial species based on their chemical and physical properties by the detection of the peptidoglycan layer

Question 2

What color is Gram positive?

Answer 2

Purple

Question 3

What color is Gram Negative?

Answer 3

Pink

Question 4

Is gram Positive a thin or thick layer?

Answer 4

Thick

Question 5

Is gram Negative a thin or thick layer?

Answer 5

Thin

Question 6

Staphylococcus epidermis, Gram + or -

Answer 6

Gram +

Question 7

Micrococcus Lutens, Gram + or -

Answer 7

Gram +

Question 8

E. Coli, Gram + or -

Answer 8

Gram -

Question 9

How many types of media?

Answer 9

1) Liquid broth
2) Agar plate
3) Agar Slant

Question 10

What are the Gram stains used? (Hint:CIS)

Answer 10

1) Crystal Violet
2) Iodine
3) Safranin

Question 11

What color would dead cells appear?

Answer 11

Blue

Question 12

What is an Endospore?

Answer 12

Thick wall structure that is formed in extreme conditions to protect the bacterias DNA

Question 13

What are the fast acid stain reagents?

Answer 13

1) Basic Fuschin
2) Acidified Alcohol
3)Methylene Blue

Question 14

What is Mycolic Acid?

Answer 14

A waxy substance that protects the cell from dehydration