week 3 exam 2 Flashcards

1
Q

What are the two main parts of photosynthesis?

A
  1. Light energy trapped and converted to chemical (light reactions)
  2. Chemical used to reduce CO2 and synthesize cell material (dark reactions)

Many phototrophs are also autotrophs.

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2
Q

What is the primary source of photosynthetic production on Earth?

A

Microbes, especially in oceans

Most of Earth’s photosynthetic production comes from these organisms.

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3
Q

What are the major light-absorbing pigments in eukaryotes and cyanobacteria?

A

Chlorophylls

These pigments play a crucial role in the light reactions of photosynthesis.

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4
Q

What are the major light-absorbing pigments in purple and green bacteria?

A

Bacteriochlorophylls

These pigments are adapted for different light conditions.

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5
Q

What is the function of accessory pigments in photosynthetic microbes?

A
  1. Transfer light energy to chlorophylls
  2. Absorb different wavelengths than chlorophylls
  3. Quench toxic forms of oxygen (photoprotection, antioxidants)

Examples include carotenoids and phycobiliproteins.

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6
Q

What are the light-harvesting arrays called that contain chlorophylls and accessory pigments in cyanobacteria and plants?

A

Photosystems

These are embedded in membranes called thylakoids.

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7
Q

How many types of photosystems are there, and what are they called?

A

Two types: Photosystem I (PSI) and Photosystem II (PSII)

These photosystems play distinct roles in the light reactions.

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8
Q

What happens when light energy is absorbed by a photosystem?

A

Energy is transferred to the Reaction Center

This process initiates the electron transport chain.

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9
Q

What occurs to chlorophyll when it absorbs light?

A

Chlorophyll electrons are excited and passed to the first acceptor in the electron transport chain (ETC)

This change is crucial for the photosynthetic process.

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10
Q

What distinguishes oxygenic photosynthesis from anoxygenic photosynthesis?

A

Oxygenic photosynthesis oxidizes H2O for electrons and forms oxygen, while anoxygenic photosynthesis uses electrons from other sources

Oxygenic photosynthesis is characteristic of eukaryotes and cyanobacteria.

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11
Q

True or False: All bacteria perform oxygenic photosynthesis.

A

False

Anoxygenic photosynthesis is performed by all other bacteria.

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12
Q

What is anabolism?

A

The synthesis of complex molecules from simpler ones with the input of energy. It uses ATP and reducing power, often in the form of NADPH.

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13
Q

Calvin Cycle?

A

An anabolic pathway for fixing CO2 into carbohydrate. It’s crucial to life and provides organic matter for heterotrophs. Reactions of photosynthesis

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14
Q

Where does the Calvin Cycle occur?

A

In chloroplasts for plants and in the cytoplasm for bacteria.

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15
Q

Name three anabolic pathways for CO2 fixation.

A

1) Calvin Cycle 2) Gluconeogenesis 3) Reductive TCA cycle (reverse Krebs/citric acid cycle)

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16
Q

What is Genotype?

A

The specific set of genes carried in the genome.

17
Q

What is Phenotype?

A

The set of observable characteristics.

18
Q

What is a Promoter?

A

The site where RNA polymerase binds to begin transcription.

19
Q

What is an Operator?

A

The site where repressor proteins bind to block transcription

20
Q

What are Inducers?

A

Molecules that bind repressors, blocking repressor binding and allowing transcription to take place.

21
Q

What is Cyclic Photophosphorylation?

A

Uses PSI, energy from ETC generates PMF to make ATP via F1F0 ATP synthase.

22
Q

What is Noncyclic Photophosphorylation?

A

Uses both PSI and PSII.

23
Q

Describe light reactions in Green and Purple Bacteria.

A

Occur in plasma membrane, use bacteriochlorophyll, anoxygenic (use H2, H2S or organic matter as electron donors), only one photosystem (PSI), only cyclic photophosphorylation (generates ATP but not NADPH).

24
Q

What is Microbial Rhodopsin?

A

A pigment protein in the plasma membrane with 7 transmembrane helices. It’s a light-driven proton pump that uses retinal to absorb light, causing conformational changes to pump protons out.

25
What experiment provided proof of DNA as genetic material in microbes?
Griffith's Transformation Experiments.
26
What are the three processes of genetic information flow
DNA replication, Transcription, and Translation
27
DNA replication
DNA replication is the process by which the genome's DNA is copied in cells
28
Translation
Translation is the process of making proteins from messenger RNA (mRNA).
29
Transcription
Transcription is the process of copying a DNA sequence into RNA. It's the first step in gene expression.
30
Gene
Functional unit of genetic information Made of deoxyribonucleic acid (DNA) - Examples: pilA, lacZ (genes), PilA, LacZ (proteins)
31
What is a genome?
All genetic material in a cell or virus - Bacterial genomes usually consist of one or more DNA chromosomes
32
What are the components of a DNA nucleotide?
Sugar (deoxyribose) - Nitrogenous base (A, G, C, or T) - Phosphate group
33
Describe DNA structure
Double helix, 2 complementary strands - Strands connected by hydrogen bonds - Base pairing rules: A with T (2 H bonds), G with C (3 H bonds)
34
How is DNA size expressed?
In base pairs (bp) - 1000 bp = 1 kilobase (kb) - E. coli chromosome: 4,640 kb or 4.64 Mbp
35
Describe DNA replication in prokaryotes
Bidirectional - From a single origin of replication (ori) - 2 forks moving in opposite directions
36
What is the major DNA replication enzyme in bacteria?
DNA Polymerase III (DNA Pol III)
37
How are many genes organized in bacteria?
As operons
38
What is the size range of bacterial genomes?
Varies greatly, from about 580 kb (e.g., Mycoplasma) to 4.64 Mbp (E. coli)