lab quiz 2

Question 1

Dye

Answer 1

Organic compound containing chomophore and auxochrome

Question 2

Chomophore and auxochrome

Answer 2

attached to benzene rings

Question 3

simple stain

Answer 3

consists of one dye that stains a component of the microbial cell

Question 4

Differential stains

Answer 4

use two or more dyes and can be used to catergorize cells into groups

Question 5

Mordant

Answer 5

chemical that fixes dye in or on a substance by combinding with the dye to form an insoluble compound

Question 6

counterstain

Answer 6

color that contrasts with primary stain (safranin)

Question 7

gram positive

Answer 7

Bacteria that retain crystal violet dye after decolorization

Question 8

gram negative

Answer 8

Bacteria that take on red color of counterstain

Question 9

gram variable

Answer 9

gram cultures must be young noit more than 12 to 18 hours as age can impact

Question 10

endospores

Answer 10

non reproductive structure produced by certain bacteria (Bacillus subtilis) during sporulation. genus Bacillus and Clostridium

Question 11

flagella

Answer 11

mordant precipitates onto the flagella to increase their diameter in size

Question 12

acid fast

Answer 12

resist decolorization with acidified alcohol, methylene blue counterstain, useful in diagnosis of tuberculosis

Question 13

metachromatic granules

Answer 13

reserves of polymerized meta phosphates/ volutin, found in baceria, diagnostic feature of Cornebacterium diphtheriae

Question 14

albert stain

Answer 14

type of differential stain used for staining metachromatic granules

Question 15

capsule/slime layer

Answer 15

exospolysaccharide surrounding the cell

Question 16

protoplast

Answer 16

bound by plasma mebrane

Question 17

Lyzosome

Answer 17

Remove cell walls in bacteria, attacks peptidoglycan

Question 18

Hyphae

Answer 18

small filaments, interwoven network of hypahe results in mycelium

Question 19

molds

Answer 19

make mycelium

Question 20

chitin

Answer 20

what the hyphae cell wall is composed oof (polysaccharide)

Question 21

sporangiospores

Answer 21

develop saclike structures at tops of hyphaew

Question 22

condiospores

Answer 22

are not enclosed within a sac and role in fungal disperal

Question 23

growth curve

Answer 23

population growht, increase number of cells in a population

Question 24

batch culture

Answer 24

when microbes ar ecultivated in a liquid medium

Question 25

lag phase

Answer 25

no immediate increase in cell number

Question 26

exponential (log phase

Answer 26

growth is constant and balanced, doubling at intervals

Question 27

stationary phase

Answer 27

growth of population is limited

Question 28

turbid

Answer 28

bacteria suspensions in a culture that scatter thge light passing through the suspension

Question 28

turbidity

Answer 28

estimates bacteriqal numbers

Question 29

spectrophotometer

Answer 29

comprised of a light source and a photocell

Question 30

unit of turbidity

Answer 30

optical density