Week 3 - Cellular Organisation II Flashcards
WEEK 2 OF CELLULAR ORGANISATION
Are proteins macromolecules
Yes
What are the building blocks of proteins
Amino Acids, Nucleic Acids
How many Amino Acids are there
20
What are chains of Amino Acids, larger then 10-15 called
Polypeptides
What are chains of Amino Acids, larger then 50 called
Proteins
What determines what kind of protein something is
the Amino Acid Sequence
What is the Amino Acid Sequence determined by
DNA
What does Organic mean
Contains Carbon
What do all Amino Acids have in their base structure
Central Carbon, An Amino Group (NH2), Carboxyl Group (COOH)
What is an Amino Group chemically made up of
NH2
What is a Carboxyl Group chemically made up of
C-O-OH
What is the R group of an Amino Acid
The unique structure of the molecule
What is the biproduct of 2 amino acids becoming a peptide bond
H2O
What is a Peptide Bond
the result of losing OH (taken from Carboxyl Group) and H (Taken from An Amino Group) after they bond, leaving CO (Carboxyl Group) to bond with NH (Amino Group)
What is ALWAYS the first amino acid in ANY Protein
Methionine
What is the start Codon
the first Amino Acid structure in a protein (always Methionine)
What is the structure of Nucleic Acids
Nucleotides
What are the 2 Classifications of Nucleic Acids
DNA (Deoxyribonucleic Acid), RNA (Ribonucleic Acid)
What is the unabbreviated name for DNA
Deoxyribonucleic Acid
What is the unabbreviated name for RNA
Ribonucleic Acid
Where is DNA found
Nucleus
What is made up of 2 Nucleotide Chains, twisted together
DNA
What is the Helical Backbone
The outer parts/structure of the Double Helix of DNA
What is the Helical Backbone made out of
Phosphate and Deoxyribose
What are the components of Nucleotides
Monosaccharides, Phosphate Groups (backbone), Nitrogenous Base
What are Nitrogenous Bases
Single or Double ring of Carbon and Nitrogen atoms
What are the 5 Nitrogenous Bases
Guanine (G), Adenine (A), Cytosine (C), Thymine (T), Uracil (U)
How many Double ring Nitrogenous Bases are their
2
How many single ring Nitrogenous bases are their
3
What are the names of the Double Ring Nitrogenous Bases
Guanine (G), Adenine (A)
What are the names of the Single Ring Nitrogenous Bases
Cytosine (C), Thymine (T), Uracil (U)
What are the Matching Pairs of Nitrogenous Bases
Adenine (A) and Thymine (T), Cytosine (C) and Guanine (G)
What does RNA stand for
Ribonucleic Acid
If you see a DNA sequence like GCUAACGUAGGC, is this DNA or RNA
RNA, Because of the Uracil
What does Uracil partner with
Adenine (A)
in RNA what does Cytosine (C) partner with
Guanine (G)
Is RNA double helix’d or Single Chained
Single Chained
Where does RNA mainly function
Cytoplasm
When does the PROTEIN SYNTHESIS Process Begin
Protein Synthesis begins when a small copy of a gene called messenger RNA (mRNA) is made from the DNA in the Nucleus
What is the process from DNA -> mRNA called
Transcription
What is the process from mRNA -> Protein called
Translation
What comes first between Translation and Transcription
Transcription
What is the name of the gene that the Transcription Process always starts on
Start Codon
What is the name of the enzyme that “unzips” the DNA to reveal the genes nitrogenous bases
Helicase
What is the Helicase
An enzyme that “unzips” the DNA double helix exposing the genes nitrogenous bases
What is the name of the enzyme that “reads” the gene code and creates a parallel molecule of mRNA
RNA Polymerase
What is RNA Polymerase
An enzyme that “reads” the gene code and creates a parallel molecule of mRNA
What happens when RNA polymerase enzyme encounters Thymine (T)
adds Adenine (A) base to newly made mRNA
What happens when RNA polymerase enzyme encounters Guanine (G)
adds Cytosine (C) base to newly made mRNA
What happens when RNA polymerase enzyme encounters Cytosine (C)
adds Guanine (G) base to newly made mRNA
What happens when RNA polymerase enzyme encounters Adenine (A)
adds Uracil (U) base to newly made mRNA
What happens in the Translation Stage
The mRNA strand produced during transcription is now read and translated into amino acid chains by ribosomes - 3 nucleotides at a time (codons)
What is a Codon
Three-bade segment of mature mRNA strand
What is the Start Codons Amino Acid equivalent (AUG)
Methionine
What’s a tRNA molecule
Transfer RNA (tRNA) is brought in when ribosomes read a codon. they then bind with onto said codon and have a complimentary ANTICODON
What is an Anticodon
3 base series complementary to the codon (found on tRNA)
What is the Anticodon to GCU
CGA
What is original codon for the ANTICODON GUG
CAC
What are the 3 STOP CODONS
UAA, UAG, UG
How long does the translation phase go on for
Until it reaches a stop codon
What is a stop codon
1 of 3 codons that ends the translation phase causing the ribosomes to end the cycle
What happens when a Ribosomes hits a Stop Codon
It releases the new protein and detaches from the mRNA
What organelle sorts proteins, by adding carbs or other components, until it finally splits from vesicles
Golgi Complex
What are the Vesicles called where the protein leaves the Golgi Complex
lysosomes
What are the 5 organelles used in protein synthesis and secretion
Nucleus, Rough ER, Ribosomes, Golgi Complex, Lysosomes
what is the structural hierarchy of a cells genes
Chromatin > Chromosomes > DNA > Genes
What are the 4 main phases of Cell Cycle
Gap 1, Synthesis, Gap 2, Mitosis
How long does the cell cycle take
Length of cell cycle varies considerably depending on cell type
What happens in the First Gap Phase
Cells complete normal tasks, Centrioles begin to get replicated
What is the sole purpose of the Synthesis Phase
DNA Replication
What is the Synthesis Phase process
Helicase splits DNA, 2 DNA Polymerase enzyme reads the base sequence (in opposite directions) on each backbone of the DNA. this creates one strand old DNA and a newly made strand per NEW DNA MOLECULE
What happens in the Second Gap Phase
Machinery getting ready for mitotic phase
What is the Interphase of the Cell Cycle
Everything but the Mitotic Phase (G1, S, G2)
What is the Mitotic Phase
The process of duplicating cells
What are the 4 Phases of Mitosis
Prophase, Metaphase, Anaphase, Telophase/Cytokinesis
What happens in the Prophase
Preparation for end goal mitosis
What happens in the Anaphase
Centrioles attach to centromeres on chromosomes and pull in half
What happens in the Metaphase
Lining chromosomes in the MIDDLE to be split
What happens in the Telophase
The creation of the 2 daughter cells, separation of DNA, Cytokinesis
What happens in Cytokinesis
The separation of the Cytoplasm
What is the end goal of mitosis
Splitting a cell into 2 daughter cells
What are Centrioles
An organelle that helps cells divide (anaphase)