WBC COUNT Flashcards
increased levels of leukocytes
Leukocytosis
white count above 11.0 x 109/L it means
Leukocytosis
counte below the normal
Leukopenia
Utilized to indicate infection and may also be employed to follow the progress of certain diseases and therapies
WBC count
DILUTING FLUIDS using WBC
1-3% acetic acid
1% hydrogen chloride
Turk’s diluting fluid
Diluting fluid of WBC is namely called
Dacie’s fluid
WBC pipette mark for blood
0.5 mark
Wipe the tip of the pipette using
Gauze
Dip the pipette into diluting fluid, and then draw the
diluting fluid into the pipette slowly, until the mixture
reaches the _____ mark..
11 mark
Discard the first _________ drops of the diluted
sample
3-4
What scanning objective are used for WBC counting
Low power objective
Read in what square
4 large squares
one large square is made up of
9 (1X1mm square)
each of WBC squares is divided further to
16 square
the center square for RBC is subdivided into
25 smaller square
the distance between each counting surface and coverslip
(depth) is
0.10 mm
the total volume of Wbc
9 cu mm
Area correction factor of WBC
1/4
Depth correction factor of WBC
10
Dilution correction factor of WBC
20
Leukocyte calculation:
WBC x area of C.f x depth c. F. x dil. C.f.
The outer line is the boundary of the
square
Double line
The middle line is the boundary of the
square
Triple line
Never count cells ________, __________, ____________
Dry, overfilled and underfilled
Do not count cells
touching
Bottom line or right line (to avoid double counting)
Counting direction used in WBC counting
Zigzag direction
What L shape is considered to be counted
Upper L shape
Sources of Errors of WBC prep
Failure to mix blood specimen
Failure to immediately mix blood specimen
Using diluent that contaminated with blood
Bubble in thoma pipette
