WBC COUNT Flashcards

1
Q

increased levels of leukocytes

A

Leukocytosis

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2
Q

white count above 11.0 x 109/L it means

A

Leukocytosis

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3
Q

counte below the normal

A

Leukopenia

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4
Q

Utilized to indicate infection and may also be employed to follow the progress of certain diseases and therapies

A

WBC count

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5
Q

DILUTING FLUIDS using WBC

A

1-3% acetic acid
1% hydrogen chloride
Turk’s diluting fluid

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6
Q

Diluting fluid of WBC is namely called

A

Dacie’s fluid

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7
Q

WBC pipette mark for blood

A

0.5 mark

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8
Q

Wipe the tip of the pipette using

A

Gauze

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9
Q

Dip the pipette into diluting fluid, and then draw the
diluting fluid into the pipette slowly, until the mixture
reaches the _____ mark..

A

11 mark

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10
Q

Discard the first _________ drops of the diluted
sample

A

3-4

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11
Q

What scanning objective are used for WBC counting

A

Low power objective

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12
Q

Read in what square

A

4 large squares

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13
Q

one large square is made up of

A

9 (1X1mm square)

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14
Q

each of WBC squares is divided further to

A

16 square

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15
Q

the center square for RBC is subdivided into

A

25 smaller square

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16
Q

the distance between each counting surface and coverslip
(depth) is

A

0.10 mm

17
Q

the total volume of Wbc

A

9 cu mm

18
Q

Area correction factor of WBC

A

1/4

19
Q

Depth correction factor of WBC

A

10

20
Q

Dilution correction factor of WBC

A

20

21
Q

Leukocyte calculation:

A

WBC x area of C.f x depth c. F. x dil. C.f.

22
Q

The outer line is the boundary of the
square

A

Double line

23
Q

The middle line is the boundary of the
square

A

Triple line

24
Q

Never count cells ________, __________, ____________

A

Dry, overfilled and underfilled

25
Q

Do not count cells
touching

A

Bottom line or right line (to avoid double counting)

26
Q

Counting direction used in WBC counting

A

Zigzag direction

27
Q

What L shape is considered to be counted

A

Upper L shape

28
Q

Sources of Errors of WBC prep

A

Failure to mix blood specimen
Failure to immediately mix blood specimen
Using diluent that contaminated with blood
Bubble in thoma pipette