Practicals Flashcards

(33 cards)

1
Q

Why do we not leave the tournique longer than 1 mins

A
  • To prevent hemoconcentration
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2
Q

3 difference ng wbc at rbc counting:

A
  • The number of square where we read
  • Different dilution is used
  • Microscopic shapes and appearance
  • Presence of nucleus
  • Difference function
  • objectives used
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3
Q

Why do we need to confirm the dietary restriction

A
  • It can show false errors within the test results
  • ensures that suitable options are available for individuals with specific dietary needs
  • their food choices have a significant impact on their health and well-being
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4
Q

What do you called gray part of two way needl

A

Rubber

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5
Q

What do you call to the flaps of butterfly

A

Wings

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6
Q

Importantance of inverted L in counting red blood cell:

A
  • Avoid double counting of cells
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7
Q

Where can you found LE cell

A

Serum

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8
Q

Why do we use reader in microhematocrit and not in macrohematocrit:

A
  • because the capillary tube that used doesn’t have any graduation label unlike macrohematocrit ut has graduation label itself where you can read right away
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9
Q

3 test used for peripheral blood smearing

A
  • wbc counting
  • rbc counting
  • differential counting
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10
Q

Supravital stain is used for

A

To stain living cells

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11
Q

The cyanmethemoglobin is said to be the method of choice for hemoglobin determination because:

A
  • Stable dilution
  • Readily available
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12
Q

different of cyanmeth and acid hematon

A
  • color comparator is used
  • Used of spectrophotometer
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13
Q

Hemoglobin results are
affected by the following
factors :

A

Age
Sex
Altitude of locality

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14
Q

Sources of Errors cyanmeth

A
  • Improper blood collection
  • Improper puncture
  • Error method
  • Error equipment
  • Imporper colometric
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15
Q

Hemoglobin determination is used to

A
  • determine anemia
  • screen for disease with anemia
  • treatment anemia
  • evaluate polycythemia
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16
Q

Hemoglobinemia
This is found :

A

severe infection
b. severe burns
c. poisoning mushroom

17
Q

Principle of acid hematin:

A

Hemoglobin is converted to acid and resulting brownish yellow color with the color standard in the comparator block

18
Q

Pipette used in acid hematin

A

Sahli pipette

19
Q

Principle of cyanmethemoglobin

A

Convert the ferrous to ferric state

20
Q

Specimen of choice acid hematin

A

Unclotted venous blood

21
Q

3 difference ng wbc at rbc pipette:

A
  • size of bulb (r: larger/ w: smaller)
  • beads (r: red/ w: white)
  • graduation mark (r: 101/ w: 11)
  • size lumen (r: smaller/ w: larger)
22
Q

The RBC count is one of the tests that are
used for the diagnosis of

A

Anemia
Polycythemia

23
Q

Counter used in rbc

A

Tally counter

24
Q

Pathologic Variation

A

• Increase erythrocyte count in polycythemia .
• Increase in pulmonary tuberculosis and
pulmonary fibrosis.
• Increase in acute poisoning
• Decrease in anemia and after hemorrhages.

25
Physiologic Variation
Increased count in dehydration • Increased count in exercise • Newborn children have higher counts than adults, • Women have lower counts than male • Individual living at higher altitudes have higher counts.
26
Pathologic Variation rbc
• Increase erythrocyte count in polycythemia . • Increase in pulmonary tuberculosis and pulmonary fibrosis. • Increase in acute poisoning • Decrease in anemia and after hemorrhages.
27
False Increase macro
Undercentrifugation Prolonged standing Inclusion buffy coat
28
False Decrease macro
overcentrifugation Hemolyzed Sample increased concentration of anticoagulant.
29
S O U RC E S O F E R RO R micro
• Incomplete sealing of the microhematocrit tubes - falsely low results • Inadequate centrifugation • Time and speed of centrifugation • Overanticoagulated
30
Esr principle
Detect and monitor inflammatory response
31
Thick blood smears is used for
Blood parasite
32
Moct common supravital stain
Brilliant cresyl blue New methylene blue
33
Principle of osmotic fragality test
ability of the red cells to take up fluid without lysing.