Practicals Flashcards

1
Q

Why do we not leave the tournique longer than 1 mins

A
  • To prevent hemoconcentration
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2
Q

3 difference ng wbc at rbc counting:

A
  • The number of square where we read
  • Different dilution is used
  • Microscopic shapes and appearance
  • Presence of nucleus
  • Difference function
  • objectives used
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3
Q

Why do we need to confirm the dietary restriction

A
  • It can show false errors within the test results
  • ensures that suitable options are available for individuals with specific dietary needs
  • their food choices have a significant impact on their health and well-being
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4
Q

What do you called gray part of two way needl

A

Rubber

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5
Q

What do you call to the flaps of butterfly

A

Wings

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6
Q

Importantance of inverted L in counting red blood cell:

A
  • Avoid double counting of cells
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7
Q

Where can you found LE cell

A

Serum

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8
Q

Why do we use reader in microhematocrit and not in macrohematocrit:

A
  • because the capillary tube that used doesn’t have any graduation label unlike macrohematocrit ut has graduation label itself where you can read right away
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9
Q

3 test used for peripheral blood smearing

A
  • wbc counting
  • rbc counting
  • differential counting
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10
Q

Supravital stain is used for

A

To stain living cells

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11
Q

The cyanmethemoglobin is said to be the method of choice for hemoglobin determination because:

A
  • Stable dilution
  • Readily available
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12
Q

different of cyanmeth and acid hematon

A
  • color comparator is used
  • Used of spectrophotometer
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13
Q

Hemoglobin results are
affected by the following
factors :

A

Age
Sex
Altitude of locality

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14
Q

Sources of Errors cyanmeth

A
  • Improper blood collection
  • Improper puncture
  • Error method
  • Error equipment
  • Imporper colometric
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15
Q

Hemoglobin determination is used to

A
  • determine anemia
  • screen for disease with anemia
  • treatment anemia
  • evaluate polycythemia
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16
Q

Hemoglobinemia
This is found :

A

severe infection
b. severe burns
c. poisoning mushroom

17
Q

Principle of acid hematin:

A

Hemoglobin is converted to acid and resulting brownish yellow color with the color standard in the comparator block

18
Q

Pipette used in acid hematin

A

Sahli pipette

19
Q

Principle of cyanmethemoglobin

A

Convert the ferrous to ferric state

20
Q

Specimen of choice acid hematin

A

Unclotted venous blood

21
Q

3 difference ng wbc at rbc pipette:

A
  • size of bulb (r: larger/ w: smaller)
  • beads (r: red/ w: white)
  • graduation mark (r: 101/ w: 11)
  • size lumen (r: smaller/ w: larger)
22
Q

The RBC count is one of the tests that are
used for the diagnosis of

A

Anemia
Polycythemia

23
Q

Counter used in rbc

A

Tally counter

24
Q

Pathologic Variation

A

• Increase erythrocyte count in polycythemia .
• Increase in pulmonary tuberculosis and
pulmonary fibrosis.
• Increase in acute poisoning
• Decrease in anemia and after hemorrhages.

25
Q

Physiologic Variation

A

Increased count in dehydration
• Increased count in exercise
• Newborn children have higher counts than
adults,
• Women have lower counts than male
• Individual living at higher altitudes have
higher counts.

26
Q

Pathologic Variation rbc

A

• Increase erythrocyte count in polycythemia .
• Increase in pulmonary tuberculosis and
pulmonary fibrosis.
• Increase in acute poisoning
• Decrease in anemia and after hemorrhages.

27
Q

False Increase macro

A

Undercentrifugation
Prolonged standing
Inclusion buffy coat

28
Q

False Decrease macro

A

overcentrifugation
Hemolyzed Sample
increased concentration of
anticoagulant.

29
Q

S O U RC E S O F E R RO R micro

A

• Incomplete sealing of the microhematocrit tubes - falsely low
results
• Inadequate centrifugation
• Time and speed of centrifugation
• Overanticoagulated

30
Q

Esr principle

A

Detect and monitor inflammatory response

31
Q

Thick blood smears is used for

A

Blood parasite

32
Q

Moct common supravital stain

A

Brilliant cresyl blue
New methylene blue

33
Q

Principle of osmotic fragality test

A

ability of the red cells to take up fluid without lysing.