Practicals Flashcards
Why do we not leave the tournique longer than 1 mins
- To prevent hemoconcentration
3 difference ng wbc at rbc counting:
- The number of square where we read
- Different dilution is used
- Microscopic shapes and appearance
- Presence of nucleus
- Difference function
- objectives used
Why do we need to confirm the dietary restriction
- It can show false errors within the test results
- ensures that suitable options are available for individuals with specific dietary needs
- their food choices have a significant impact on their health and well-being
What do you called gray part of two way needl
Rubber
What do you call to the flaps of butterfly
Wings
Importantance of inverted L in counting red blood cell:
- Avoid double counting of cells
Where can you found LE cell
Serum
Why do we use reader in microhematocrit and not in macrohematocrit:
- because the capillary tube that used doesn’t have any graduation label unlike macrohematocrit ut has graduation label itself where you can read right away
3 test used for peripheral blood smearing
- wbc counting
- rbc counting
- differential counting
Supravital stain is used for
To stain living cells
The cyanmethemoglobin is said to be the method of choice for hemoglobin determination because:
- Stable dilution
- Readily available
different of cyanmeth and acid hematon
- color comparator is used
- Used of spectrophotometer
Hemoglobin results are
affected by the following
factors :
Age
Sex
Altitude of locality
Sources of Errors cyanmeth
- Improper blood collection
- Improper puncture
- Error method
- Error equipment
- Imporper colometric
Hemoglobin determination is used to
- determine anemia
- screen for disease with anemia
- treatment anemia
- evaluate polycythemia
Hemoglobinemia
This is found :
severe infection
b. severe burns
c. poisoning mushroom
Principle of acid hematin:
Hemoglobin is converted to acid and resulting brownish yellow color with the color standard in the comparator block
Pipette used in acid hematin
Sahli pipette
Principle of cyanmethemoglobin
Convert the ferrous to ferric state
Specimen of choice acid hematin
Unclotted venous blood
3 difference ng wbc at rbc pipette:
- size of bulb (r: larger/ w: smaller)
- beads (r: red/ w: white)
- graduation mark (r: 101/ w: 11)
- size lumen (r: smaller/ w: larger)
The RBC count is one of the tests that are
used for the diagnosis of
Anemia
Polycythemia
Counter used in rbc
Tally counter
Pathologic Variation
• Increase erythrocyte count in polycythemia .
• Increase in pulmonary tuberculosis and
pulmonary fibrosis.
• Increase in acute poisoning
• Decrease in anemia and after hemorrhages.
Physiologic Variation
Increased count in dehydration
• Increased count in exercise
• Newborn children have higher counts than
adults,
• Women have lower counts than male
• Individual living at higher altitudes have
higher counts.
Pathologic Variation rbc
• Increase erythrocyte count in polycythemia .
• Increase in pulmonary tuberculosis and
pulmonary fibrosis.
• Increase in acute poisoning
• Decrease in anemia and after hemorrhages.
False Increase macro
Undercentrifugation
Prolonged standing
Inclusion buffy coat
False Decrease macro
overcentrifugation
Hemolyzed Sample
increased concentration of
anticoagulant.
S O U RC E S O F E R RO R micro
• Incomplete sealing of the microhematocrit tubes - falsely low
results
• Inadequate centrifugation
• Time and speed of centrifugation
• Overanticoagulated
Esr principle
Detect and monitor inflammatory response
Thick blood smears is used for
Blood parasite
Moct common supravital stain
Brilliant cresyl blue
New methylene blue
Principle of osmotic fragality test
ability of the red cells to take up fluid without lysing.
