Leukocytes Flashcards
STAB NEUTROPHIL
Diameter:
9-15um
STAB NEUTROPHIL
Cytoplasm:
Granules are pinker and numerous
STAB NEUTROPHIL
Nucleus:
Elongated and band shaped
SEGMENTED NEUTROPHIL
Diameter:
9-15um
SEGMENTED NEUTROPHIL
Cytoplasm:
Abundant, Pink to rose violet granules
SEGMENTED NEUTROPHIL
Nucleus:
2-5 lobes thin filament
EOSINOPHIL
Diameter:
9-15um
EOSINOPHIL
Cytoplasm:
reddish- orange granules
EOSINOPHIL
Nucleus:
2 lobes
BASOPHIL
Diameter:
10-16um
BASOPHILS
cytoplasm:
Bluish-black granules (fewer granules)
BASOPHILS
Nucleus:
rarely 3-4 lobes
SMALL LYMPHOCYTES
diameter:
8-10um
SMALLYMPHOCYTES
Cytoplasm:
moderate to dark blue
SMALL LYMPHOCYTE
Nucleus:
Round or oval in shape
MEDIUM LYMPHOCYTE
diameter:
10-12um
MEDIUM LYMPHOCYTE
cytoplasm:
Most abundant; pale to moderately blue
MEDIUM LYMPHOCYTE
nucleus:
Round or oval shape
LARGE LYMPHOCYTE
Diameter:
12-16um
LARGE LYMPHOCYTE
Cytoplasm:
Abundant; very pale blue
LARGE LYMPHOCYTE
nucleus:
Round or oval shape
MONOCYTE
diameter:
14-20um
MONOCYTES
cytoplasm:
Abundant, blue gray
MONOCYTES
nucleus:
Round, kidney shape or folded over
Ref range neutrophil
37-77%
Ref range band
0-11%
Ref range lymphocytes
10-44%
Ref range monocyte
2-10%
Ref range eosinophil
0-7%
Ref range basophil
0-1%
Function of neutrophil
Phagocytosis
Function of lymphocytes
Production of antibody
Function of eosinophil
Detect allergen, parasitic and fungal infection
Function of basophil
Detect allergen and parasitic infection
Leukocytes differential count express in
%, uL and cells/L
Length of sample should be
2/3
Oil used
Cedar wood oil
Machine or apparatus used in counting
Cell counter
Stain used in leukocyte differential count
Wright’s stain
Composition of wright’s stain
Fixative
Eosin stain
New methylene blue
How many dip in each stain
5 dips
Counting methods
Cross-sectional
Longitudinal
Battlement
Observing direction
Snake like direction
absolute increase in the number of neutrophils
Neutrophilia
Population order
Never Let Monkey Eat Banana
Size order
Monocytes
Large lymphocytes
Basophil
Medium lymphocytes
Eosinophil
Stab neutrophil
Segmented neutrophil
Small lymphocytes
NEUTROPHILIA
Appendicitis
Myelogenous
leukemia
Bacterial infection
EOSINOPHILIA
Allergic reactions
› Scarlet fever
› Parasitic infection
› Eosinophilic leukemia
LYMPHOCYTOSIS
Viral infection
› Whooping cough
› Infectious mononucleosis
› Lymphocytic leukemia
MONOCYTOSIS
Tuberculosis
Typhoid
Brucellosis
Collagen disease