W12 & 13 Quiz Flashcards

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1
Q

What does the USDA stand for?

A

US Department of Agriculture

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2
Q

What does the FDA stand for?

A

Food and Drug Administration

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3
Q

What does the EPA stand for?

A

Environmental Protection Agency

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4
Q

Which three government organizations oversee GMO foods?

A

The FDA, EPA, and the USDA

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5
Q

What are the cons or the hardships when bringing GMO’s to market?

A

All GMOS have to meet the same safety standard, they need mandatory labels, extensive safety evaluations, they can sometimes be considered an invasive species through pollination

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6
Q

What pros are there and what is the process to bring GMO’s be brought to market?

A

They don’t usually cost more and they are not necessarily more/less healthy.No more likely have allergies or be toxic however allergies are watched.

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7
Q

Arguments against GMO’s

A

Farmers can’t compete with large businesses that do GMOs, Ethical feelings about not playing God, No trust in government, people don’t know about long term consequences, New allergens could be introduced that could cause problems. Not enough transparency. They are scared about viruses mutating.

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8
Q

Arguments for GMO’s

A

Farmers will lose less plants, it’s faster, you can choose genes from the same species, virus resistance, more nutrient-rich. Food scarcity could be easier. You don’t have to use as many pesticides.

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9
Q

What are approved transgenics?

A

Many GMO crops are approved and used to make ingredients that Americans eat such as cornstarch, corn syrup, corn oil, soybean oil, canola oil, or granulated sugar. A few fresh fruit and vegetables are available in GMO varieties, including potatoes, summer squash, apples, papayas, and pink pineapples.

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10
Q

Corn is the highest and contains the most varieties that are approved as genetically modified. True or False.

A

True. We use a lot of corn in this country including from corn syrup, etc

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11
Q

What is flavor savr?

A

First GMO that was eaten. a genetically modified tomato, CGN-89564-. was the first commercially grown genetically engineered food to be granted a license for human consumption. It was developed by the Californian company Calgene in the 1980s. The tomato has an improved shelf-life, increased fungal resistance and a slightly increased viscosity compared to its non-modified counterpart.he Flavr Savr contains two genes added by Calgene; a reversed antisense polygalacturonase gene which inhibits the production of the aforementioned rotting enzyme and a gene responsible for the creation of APH(3’)II,[3] which confers resistance to certain aminoglycoside antibiotics including kanamycin and neomycin.

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12
Q

What are bovine cows?

A

milk that contains human antibodies. Human antibodies made in genetically engineered cows have proved safe in an early stage clinical trial, U.S. scientists said it could be developed into a treatment for the fatal viral disease, MERS.

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13
Q

What was AquaAdvantage salmon?

A

they don’t get bigger than natural salmon but they mature rapidly. higher food source. AquAdvantage® Salmon was developed by inserting into a fertilized Atlantic salmon egg, a transgene constructed from the growth hormone gene of a related species.

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14
Q

What was the enviro pig?

A

It was the first gmo animal approved by the FDA. Enviropig™ is genetically engineered to produce the enzyme phytase in its salivary glands to enable more effective digestion of phytate, the from of phosphorus found in pig feed ingredients like corn and soybeans.

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15
Q

What was selective breeding with plants and animals?

A

That has been happening for a very long time. Certains breeds of plants are bred with other plants. Same with animals and dogs but GMO’s are different and they are relevantly new. it’s finding a gene in another organism that we like and putting into the orgamism of interest.

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16
Q

Define GMO’s

A

finding a gene in another organism that we like and putting it into the orgamism of interest.

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17
Q

What are the kinds of resistence that we are trying to improve with GMO

A

Vitamins, Immune resistence, viral resistence,

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18
Q

Explain the Soy bean with herbicide resistence

A

Transgenic soybeans carry multiple Bt genes that provide resistance to important insect pests in agricultural crops have been produced. Bt proteins can suppress major pests thus reducing insecticide

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19
Q

What virus were Plums with viral resistence protecting from?

A

The HoneySweet plum has been genetically modified to be resistant to the plum pox virus (PPV).

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20
Q

Describe what the point is for Corn with higher oil content

A

The first high-oil corn varieties were selected in 1896 at the University of Illinois. High-oil corn is a variety of corn that has been genetically selected to contain a greater oil (fat) content in the kernel than would be contained in current “standard” varieties of corn. This is from a larger germ of the corn.

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21
Q

Explain BT maize

A

Bt maize has revolutionized pest control and many farmers have benefited, but some people remain skeptical of this new technology.Bacillus thuringiensis is a species of bacteria that produces proteins that are toxic to certain insects. Because of this, it has been used as a safe microbial insecticide for over 50 years to control pest caterpillars.

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22
Q

What is atryn?

A

goat milk for treating blood clots. ATryn was developed by a Massachusetts biotechnology company, GTC Biotherapeutics, by altering the genes of goats so they would produce milk rich in antithrombin, a protein that in humans acts as a natural blood thinner

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23
Q

BT corn. Talk about this and the controversy

A

There was worry it could affect monarch butterflies but then later studies debunked that and it was approved by the FDA. Critics are worried that it can also kill beneficial insects.

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24
Q

How do the GMO’s work for plants. the process

A

They isolate the gene of interest, cut out restriction enzymes, ligate into cloning vector, insert the vector into the plant cells, select for plants that took up the vector, and then use those cells to grow a plant with Acrobacterium or a Gene Gun.

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25
Q

What is a gene gun?

A

You can insert the cloned restriction enzyme into the plant using a gene gun

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26
Q

How does the GMO work for animals

A

Using transduction with viral vectors. There is some unpredictability with it. It’s a different system then with plants.

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27
Q

What is transduction

A

The process in which a virus transfers genetic material from one bacterium to another.

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28
Q

Which viruses are used most?

A

adenovirus (AAV), adino- associated viruses (AAV2), retro viruses.

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29
Q

Retroviruses use RNA for their information. True or False

A

True

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30
Q

What is Crispr Cas9 Nuclease?

A

It’s a gene targeting technique that is faster, less expensive, and it is more accurate. It comes from bacteria and they use it as the immune system. The Cas9 is an enzyme. It’s a nuclease and a helicase. It cuts double stranded DNA. It is It attached to CRISPR which is an RNA molecule which guides the enzyme to cut. It has a specific sequence. The crispr binds to a specific sequence. The Cas9 nucleas binds to the guide and then the Cas9 cuts both strands and then the cell recognizes the damanged dna and repairs it The Crispr CAS9 doesnt repair. It just cuts it but the cell repairs it and so it can inactivate a gene that you don’t want to be active anymore. It can also fix damaged genes or introduce a new gene sequence.

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31
Q

Explain human gene editing

A

Gene editing is not new but it is expensive with a high rate of error.

32
Q

What does CRISPR stand for?

A

Clustered Regularly Interspaced Short Palindromic Repeats

33
Q

Crispr is not palendromic. True or False.

A

True. It is.

34
Q

What does CAS-9 stand for?

A

Crispr associated standard protein.

35
Q

What is Tracr DNA?

A

it holds the crispr RNA in place

36
Q

Where was CRISPR first identified?

A

e.coli

37
Q

What will Helicases dna do on the Cas proteins?

A

It will unwind the dna

38
Q

What will the nucleases on the CAS proteins during CRISPR do?

A

It will cut the dna

39
Q

How was streptoccos pygonenes?

A

This is the cas protein virus that they worked on and connected and creating crispr RNA and they have tracer RNA with something called chimera. It allows more control of where we cut as well. We can break, insert, and repair genes.

40
Q

What is chimera?

A

A chimera is essentially a single organism that’s made up of cells from two or more “individuals”

41
Q

what is a crispr system?

A

a crispr system is an immune system that was identified in bacteria and then modified in humans The CRISPR method is based on a natural system used by bacteria to protect themselves from infection by viruses but brought to a clinic and modified using RNA and DNA.

42
Q

True or False: CRISPR-Cas9 has a lot of potential as a tool for treating a range of medical conditions that have a genetic component, including cancer?, hepatitis B or even high cholesterol.

A

True

43
Q

The earliest method scientists used to edit genomes in living cells was called homologous recombination. T or F

A

True

44
Q

In what year range did the researchers started using zinc-finger nucleases (ZFN) to improve the specificity of genome editing and reduce off-target edits.

A

1990’s

45
Q

In 2009, a new class of proteins called Transcription Activator-Like Effector Nucleases (TALENs) arrived to the genome editing scene. True or False.

A

True

46
Q

While TALENs and ZFNs are comparable in terms of how efficiently they can create edits to the genome, ZFN’s bear the advantage of greater simplicity

A

False, that is TALENS have greater simplicity.

47
Q

CRISPR can make deletions in the genome and/or be engineered to insert new DNA sequences. True or False

A

True

48
Q

Over the past few years, researchers studying the crispr system realized that it could be engineered to cut not just viral DNA, but any DNA sequence at a precisely chosen location by changing the guide RNA to match the target.

A

True

49
Q

What elements does Crispr CASR-9 consist of?

A

crRNA, tracrRNA, and Cas9 protein

50
Q

Jennifer Doudna and Emmanuelle Charpentier in 2012 discovered a new level of genome targeting that is both efficient and easy to use. It requires the use of the Cas9 nuclease protein. True or false

A

true

51
Q

What does the nuclease part of Cas9 do?

A

Cas9 is a nuclease enzyme, that simply means it cuts double stranded DNA and produces breaks that must be repaired by the DNA repair system of the cell. When repair occurs, it usually leads to damage that inactivates a gene so no functional protein is made.

52
Q

What is genome editing?

A

Genome editing refers to the ability to add or delete DNA bases on a chromosome. When we specifically target a gene, then we refer to it as gene editing.

53
Q

Why are we interested in editing genes?

A

We are interested in editing genes because it allows us to determine what will happen to a cell when a protein is no longer made. In animal cells, we can produce animal models of human disease for testing drugs of interest.

54
Q

What 2 different RNAs, when combined, make up the guide RNA?

A

sgRNA (single guide) and CAS9 protein

55
Q

Explain how sgRNA and Cas9 work together to introduce changes into DNA.

A

They target specific cleavage and cut the gene. They used the PAM sequence and they rely on the cellular error prone repair to knock the gene out.

56
Q

With all of the good that gene editing can do, why do some believe it is a dangerous proposal?

A

There are ethical issues, and safety issues for altering life, especially human life.

57
Q

What are the two types of cloning?

A

Therapeutic cloning and reproductive cloning

58
Q

What is therapeutic cloning?

A

It is the creation of pluripotent stem cells using somatic cells. It is used to treat organs or tissues to cure diseases and treat injuries.

59
Q

What is reproductive cloning?

A

it is creating offspring asexually (making a whole new identical copy).

60
Q

What are the two methods used in cloning?

A

Artificial Twinning using embryos and Somatic Cell Nuclear Transfer

61
Q

What is another name for artificial twinning?

A

Blastomere Separation

62
Q

Explain the process of artificial twinning

A

the cells separate, each cell develops into an identical embryo, the embryo is implanted into a surrogate mother, identical cloned offspring is born

63
Q

Name examples of animals cloned using twinning

A

1885 sea urchins, 1902 salamanders,

64
Q

Name examples of animals cloned using nuclear transfer

A

1952 frog, 1975 rabbit, 1984 sheep, 1987 cow

65
Q

How does nuclear transfer work for cloning

A

they took the nucleus from an early embryo and transferred it into a single cell from which the original nucleus had been removed.

66
Q

When was somatic cell nuclear transfer introduced for the first time?

A

In 1997 with dolly the sheep

67
Q

What is the difference between therapeutic and reproductive cloning?

A

reproductive cloning is when you implant the embryo into the sheep or animal or plant. therapeutic cloning is when you remove stem cells from the embryo and grow in a culture instead.

68
Q

What are the advantages of cloning?

A

the production of animal models for disease studies, producing stem cells, producing of drugs from transgenic animals, it could also increase the efficiency of livestock population. It may also be possible revive endangered species

69
Q

What are the disadvantages of cloning?

A

There would be a decline in genetic diversity, we would be taking nature into our own hands and playing God, there may be other ethical concerns, there may be physical defects, or emotional/mental problems of the clone. 95% failure rate does lead to harm to animals that are being tested. There may be no benefit to consumers.

70
Q

What are some risks with whole animal cloning?

A

There is a high failure rate, large offspring syndrome (LOS), defects in vital organs, premature aging, and immune system difficulties

71
Q

What animals have been cloned using whole animal cloning?

A

Sheep, cow, mouse, pig, goat, monkey, cat, cattle, euro sheep, mule, deer, rabbit, horse, dog, water buffalo, ferret, camel, ibex, and coyote.

72
Q

Which extinct species have they cloned?

A

A passenger pigeon, the pyrenean ibex, Tasmanian tiger, and the auroch.

73
Q

Is cloning humans different procedurally from cloning animals?

A

The basic procedures for cloning humans are the same as for cloning animals.

74
Q

Are there any physiological risks to cloning humans?

A

In each step of the process, there is a risk associated with human error and improper conditions.

75
Q

Are these risks different for animals?

A

The cell differentiation process is species specific, and we do not understand it well in humans.
Most mammal clones are miscarried or born with severe deformities that result in a short life span.

76
Q

Genetics does not completely define a person. True or False

A

True. this is an ethics concern for cloning humans or animals