Practice Quiz Flashcards

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1
Q

Which bacteria is used for inserting clone plasmids into a plant cell?

A

Agrobacterium tumefaciens

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2
Q

What does crRNA + tracrRNA equal

A

gRNA

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3
Q

What does gRNA + Cas9 equal

A

CRISPR-Cas9 Gene Editing System

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4
Q
  1. Which part of the gRNA molecule serves as the template that binds to the target DNA sequence?
A

crRNA

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5
Q

Which group of viral vectors is promising for its ability to infect dividing and non-dividing cells and not trigger an immune response in the patient, but has limited ability to carry larger human genes due to its small size.

A

Adeno-Associated Virus

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6
Q

Cas9 requires a gRNA molecule in order to know where to cut. True or False

A

True

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7
Q

Cas9 cuts with sticky ends. True or False

A

False. It cuts with blunt ends.

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8
Q

What is the purpose of transferring a somatic cell nucleus into an enucleated zygote during the process of somatic cell nuclear transfer?

A

To create a cell capable of developing into a whole organism that also contains the genetic instructions of the adult to be cloned.

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9
Q

Which factors play a part in ensuring that a calico cat and its clone will be phenotypically different from each other?

A

environmental factors, mitochondrial dna, and x inactivation

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10
Q

Lentivirus vectors have the ability to make permenant changes to the target cell’s genome. True or False

A

true

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11
Q

What is the role of Cas9 in the CRISPR-Cas9 gene editing process?

A

To cleave the target DNA sequence at the site specified by the gRNA

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12
Q
  1. Which methodsare used to insert a foreign gene into a host animal cell?
A

Electroporation
Microinjection
Transduction

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13
Q

What issue(s) were scientists trying to resolve by the creation of the Flavr-Savr Tomato?

A

Tomatoes often become mushy before producers can effectively sell them to consumers

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