Practice Quiz

Question 1

Which bacteria is used for inserting clone plasmids into a plant cell?

Answer 1

Agrobacterium tumefaciens

Question 2

What does crRNA + tracrRNA equal

Answer 2

gRNA

Question 3

What does gRNA + Cas9 equal

Answer 3

CRISPR-Cas9 Gene Editing System

Question 4

2. Which part of the gRNA molecule serves as the template that binds to the target DNA sequence?

Answer 4

crRNA

Question 5

Which group of viral vectors is promising for its ability to infect dividing and non-dividing cells and not trigger an immune response in the patient, but has limited ability to carry larger human genes due to its small size.

Answer 5

Adeno-Associated Virus

Question 6

Cas9 requires a gRNA molecule in order to know where to cut. True or False

Answer 6

True

Question 7

Cas9 cuts with sticky ends. True or False

Answer 7

False. It cuts with blunt ends.

Question 8

What is the purpose of transferring a somatic cell nucleus into an enucleated zygote during the process of somatic cell nuclear transfer?

Answer 8

To create a cell capable of developing into a whole organism that also contains the genetic instructions of the adult to be cloned.

Question 9

Which factors play a part in ensuring that a calico cat and its clone will be phenotypically different from each other?

Answer 9

environmental factors, mitochondrial dna, and x inactivation

Question 10

Lentivirus vectors have the ability to make permenant changes to the target cell’s genome. True or False

Answer 10

true

Question 11

What is the role of Cas9 in the CRISPR-Cas9 gene editing process?

Answer 11

To cleave the target DNA sequence at the site specified by the gRNA

Question 12

21. Which methodsare used to insert a foreign gene into a host animal cell?

Answer 12

Electroporation
Microinjection
Transduction

Question 13

What issue(s) were scientists trying to resolve by the creation of the Flavr-Savr Tomato?

Answer 13

Tomatoes often become mushy before producers can effectively sell them to consumers