W11 Gene tech Flashcards
What is the first step in genetic technology processes
DNA extraction and isolating DNA from cells
What is the purpose of agarose gel electrophoresis
separate DNA fragments based on size
Define restriction mapping
technique to analyse DNA by cutting it with specific restriction enzymes
What is molecular cloning
creating the same copies of a DNA fragment
What is a multiple cloning site (MCS) in molecular cloning
A region in a plasmid containing multiple restriction sites
What is Sanger sequencing
sequencing method using chain-termination
what is NGS used for
genome analysis
Define whole exome sequencing
sequencing only the protein-coding regions of a genome
What is transcriptomics (RNA-seq)
analyse gene expression of RNA molecules
What does CRISPR-Cas9 do
cause DDSB
What is the role of Cas13 in CRISPR tech
targets RNA instead of DNA seq
What can dCas13a be used for
To label RNA molecules without cutting them
what can CRISPRa and CRISPRi be used for
CRISPRa = promote gene expression
CRISPRi = inhibit gene expression
what are the steps to cloning bacteria
Amplify DNA using PCR
Digest plasmid and PCR sample with the same restriction enzyme
Insert DNA into plasmid using DNA ligase
Insert plasmid into bacteria
Grow on agar plate with correct antibiotic
what are the steps to Sanger Sequencing
PCR with fluorescent ddNTPs
separate using capillary gel electrophoresis
use laser excitation
detected by sequencing machine
