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Veterinary Medicine > VMB : Bacillus > Flashcards

VMB : Bacillus Flashcards

1
Q

Bacillus are gram what

A

Bacillus is positive.. Kinda sound like zealous so it’s positive yeh

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2
Q

Bacillus are gram positive except

A

Bacillus macquarensis

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3
Q

Are they spore forming, true or false

A

True

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4
Q

Bacillus are motile with the aid of peritrichous flagella except

A

Bacillus anthracis

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5
Q

Bacillus Megaterium is associated with

A

Food poisoning

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6
Q

Which Bacillus is used for biological pest control

A

Bacillus thuringiensis

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7
Q

Bacillus ___ is associated with food poisoning

A

Megaterium

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8
Q

Bacillus ___ is associated with food poisoning

A

Megaterium

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9
Q

Anthrax occurs when bacillus anthracis endospore enter a host through ___ and ___

A

Inhalation, ingestion, abrasion on the skin

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10
Q

Anthrax occurs when bacillus anthracis endospore enter a host through ___ and ___

A

Inhalation, ingestion, abrasion on the skin

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11
Q

Bacillus __ can cause anthrax

A

Anthracis

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12
Q

Bacillus anthracis virulence depends on

A

Production of capsules and spores

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13
Q

What is a symptom of anthracis

A

The blood is dark, tarry and non clotting with bleeding from natural orifices

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14
Q

What is a symptom of anthracis

A

The blood is dark, tarry and non clotting with bleeding from natural orifices

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15
Q

Horses that have anthrax have what symptoms

A

Colic, diarrhoea,oedema

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16
Q

Swine bacillus anthracis manifest as

A

Localized pharyngeal infection with regional lymphadenitis, ulcerative enteritis and mesenteric lymphadenitis may occur

17
Q

Human symptoms of anthracis

A

Infection through the skin produces Malignant carbuncles or pustules
Through inhalation produces pulmonary edema, haemorrhagic pneumonia and meningitis

18
Q

Anthrax can be demonstrated by what stains

A

McFadyean or Giemsa or Wright stain

19
Q

Anthrax can be demonstrated by what stains

A

McFadyean or Giemsa or Wright stain

20
Q

McFadyean reaction gives

A

Bluish rod surrouded by pink and purple capsule💦

21
Q

McFadyean reaction

A

Bluish rod surrouded by purplish pink capsule
The organisms may appear singly or in pairs or long chains with unstained central areas representing spores

22
Q

McFadyean reaction

A

Bluish rod surrouded by purplish pink capsule
The organisms may appear singly or in pairs or long chains with unstained central areas representing spores

23
Q

Steps involved in culturing anthracis

A
  1. Homogenize tissue using physiological saline or broth as a diluent
  2. Inoculate blood agar from suspension,blood, swaps and biopsies
    3.inoculate aerobically at 37°C For 24 hours
  3. Large flat and rough colonies with irregular edges which are usually non hemolytic
  4. Some areas called medusa head type or judges wig type colonies
    On nutrient agar colonies are dull, opaque and grayish white with irregular borders 🌚💀
24
Q

Steps involved in culturing anthracis

A
  1. Homogenize tissue using physiological saline or broth as a diluent
  2. Inoculate blood agar from suspension,blood, swaps and biopsies
    3.inoculate aerobically at 37°C For 24 hours
  3. Large flat and rough colonies with irregular edges which are usually non hemolytic
  4. Some areas called medusa head type or judges wig type colonies
    On nutrient agar colonies are dull, opaque and grayish white with irregular borders 🌚💀
25
Q

Selective agar for Bacillus anthracis

A

PLET
Polymyxin lysozyme EDTA thallous acetate

26
Q

Differential diagnosis of anthracis

A

Acute poisoning
Snake bite
Septicemia

27
Q

Bacillus cereus produces two types of toxin?

A

Diarrhoeal and emetic

28
Q

Name two selective media for Bacillus cereus

A

Kim and Goepfert (KG) agar
Mannitol egg yolk polymyxin agar MYP

29
Q

What appearance is Bacillus colony

A

Medusa head or judge head

Veterinary Medicine (7 decks)

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