VMB : Bacillus

Question 1

Bacillus are gram what

Answer 1

Bacillus is positive.. Kinda sound like zealous so it’s positive yeh

Question 2

Bacillus are gram positive except

Answer 2

Bacillus macquarensis

Question 3

Are they spore forming, true or false

Answer 3

True

Question 4

Bacillus are motile with the aid of peritrichous flagella except

Answer 4

Bacillus anthracis

Question 5

Bacillus Megaterium is associated with

Answer 5

Food poisoning

Question 6

Which Bacillus is used for biological pest control

Answer 6

Bacillus thuringiensis

Question 7

Bacillus ___ is associated with food poisoning

Answer 7

Megaterium

Question 8

Bacillus ___ is associated with food poisoning

Answer 8

Megaterium

Question 9

Anthrax occurs when bacillus anthracis endospore enter a host through ___ and ___

Answer 9

Inhalation, ingestion, abrasion on the skin

Question 10

Anthrax occurs when bacillus anthracis endospore enter a host through ___ and ___

Answer 10

Inhalation, ingestion, abrasion on the skin

Question 11

Bacillus __ can cause anthrax

Answer 11

Anthracis

Question 12

Bacillus anthracis virulence depends on

Answer 12

Production of capsules and spores

Question 13

What is a symptom of anthracis

Answer 13

The blood is dark, tarry and non clotting with bleeding from natural orifices

Question 14

What is a symptom of anthracis

Answer 14

The blood is dark, tarry and non clotting with bleeding from natural orifices

Question 15

Horses that have anthrax have what symptoms

Answer 15

Colic, diarrhoea,oedema

Question 16

Swine bacillus anthracis manifest as

Answer 16

Localized pharyngeal infection with regional lymphadenitis, ulcerative enteritis and mesenteric lymphadenitis may occur

Question 17

Human symptoms of anthracis

Answer 17

Infection through the skin produces Malignant carbuncles or pustules
Through inhalation produces pulmonary edema, haemorrhagic pneumonia and meningitis

Question 18

Anthrax can be demonstrated by what stains

Answer 18

McFadyean or Giemsa or Wright stain

Question 19

Anthrax can be demonstrated by what stains

Answer 19

McFadyean or Giemsa or Wright stain

Question 20

McFadyean reaction gives

Answer 20

Bluish rod surrouded by pink and purple capsule💦

Question 21

McFadyean reaction

Answer 21

Bluish rod surrouded by purplish pink capsule
The organisms may appear singly or in pairs or long chains with unstained central areas representing spores

Question 22

McFadyean reaction

Answer 22

Bluish rod surrouded by purplish pink capsule
The organisms may appear singly or in pairs or long chains with unstained central areas representing spores

Question 23

Steps involved in culturing anthracis

Answer 23

1. Homogenize tissue using physiological saline or broth as a diluent
2. Inoculate blood agar from suspension,blood, swaps and biopsies
   3.inoculate aerobically at 37°C For 24 hours
3. Large flat and rough colonies with irregular edges which are usually non hemolytic
4. Some areas called medusa head type or judges wig type colonies
   On nutrient agar colonies are dull, opaque and grayish white with irregular borders 🌚💀

Question 24

Steps involved in culturing anthracis

Answer 24

1. Homogenize tissue using physiological saline or broth as a diluent
2. Inoculate blood agar from suspension,blood, swaps and biopsies
   3.inoculate aerobically at 37°C For 24 hours
3. Large flat and rough colonies with irregular edges which are usually non hemolytic
4. Some areas called medusa head type or judges wig type colonies
   On nutrient agar colonies are dull, opaque and grayish white with irregular borders 🌚💀

Question 25

Selective agar for Bacillus anthracis

Answer 25

PLET
Polymyxin lysozyme EDTA thallous acetate

Question 26

Differential diagnosis of anthracis

Answer 26

Acute poisoning
Snake bite
Septicemia

Question 27

Bacillus cereus produces two types of toxin?

Answer 27

Diarrhoeal and emetic

Question 28

Name two selective media for Bacillus cereus

Answer 28

Kim and Goepfert (KG) agar
Mannitol egg yolk polymyxin agar MYP

Question 29

What appearance is Bacillus colony

Answer 29

Medusa head or judge head