Viral Structure and Replication

Question 1

Protein subunits that make up the capsid

Answer 1

Capsomer

Question 2

Put the following in order for steps in replication:

1. Replication of viral DNA
2. Assembly of virions
3. Uncoating
4. Txn of late mRNA
5. Attachment
6. Release
7. Translation of late proteins
8. Penetration
9. Txn of early mRNA
10. Translation of early proteins

Answer 2

1. Attachment
2. Penetration
3. Uncoating
4. Txn of early mRNA
5. Translation of early proteins
6. Replication of viral DNA
7. Txn of late mRNA
8. Translation of late proteins
9. Assembly of virions
10. Release

Question 3

Where do DNA viruses replicate? RNA viruses?

Answer 3

DNA= nucleus
RNA= cytoplasm

Question 4

HIV
Family?
Receptor?

Answer 4

HIV
Family= Retroviridae
Receptor= CCR5, CCR3, CXCR4, Cd4

Question 5

Human rhinovirus 14
Family?
Receptor?

Answer 5

Human rhinovirus 14
Family= PICORNAVIRIDAE
Receptor= ICAM-1

Question 6

Foot and mouth disease virus (wild type)
Family?
Receptor?

Answer 6

Foot and mouth disease virus (wild type)
Family? PICORNAVIRIDAE
Receptor? VARIOUS INTEGRINS

Question 7

Foot and mouth disease virus (cell culture adapted)
Family?
Receptor?

Answer 7

Foot and mouth disease virus (cell culture adapted)
Family? PICORNAVIRIDAE
Receptor? HEPARAN SULFATE PROTEOGLYCAN

Question 8

Influenza A virus
Family?
Receptor?

Answer 8

Influenza A virus
Family? ORTHOMYXOVIRIDAE
Receptor? SIALIC ACID

Question 9

Rabies virus
Family?
Receptor?

Answer 9

Rabies virus
Family? RHABDOVIRIDIAE
Receptor? ACETYLCHOLINE, NCAM (NEURAL ADHESION MOLECULE)

Question 10

What is the mechanism for viral maturation and release in non-enveloped viruses? Enveloped viruses?

Answer 10

Non= Cell lysis
Enveloped= Budding

Question 11

Genome: dsDNA, 1 linear
Envelope: +
Morphology: pleomorphic
Virion Size: 250 x 200 x 200 (BIG)

Answer 11

Poxviridae

Question 12

Iridoviridae
Genome: 
Envelope: 
Morphology: 
Virion Size (diameter [nM]):

Answer 12

Iridoviridae
Genome: dsDNA 1 linear
Envelope: +/-
Morphology: ISOMETRIC
Virion Size (diameter [nM]): 130-300

Question 13

ASFARVIRIDAE
Genome: 
Envelope: 
Morphology: 
Virion Size (diameter [nM]):

Answer 13

ASFARVIRIDAE
Genome: dsDNA 1 linear
Envelope: +
Morphology: SPHERICAL 
Virion Size (diameter [nM]): 173-215

Question 14

HERPESVIRIDAE
Genome: 
Envelope:  
Morphology: 
Virion Size (diameter [nM]):

Answer 14

HERPESVIRIDAE
Genome: dsDNA 1 linear
Envelope:  +
Morphology: ISOMETRIC
Virion Size (diameter [nM]): 150

Question 15

ADENOVIRIDAE
Genome:
Envelope:  
Morphology:
Virion Size (diameter [nM]):

Answer 15

ADENOVIRIDAE
Genome: dsDNA 1 linear
Envelope:  NO
Morphology: ISOMETRIC
Virion Size (diameter [nM]): 80-100

Question 16

POLYOMAVIRIDAE
Genome: 
Envelope:  
Morphology:
Virion Size (diameter [nM]):

Answer 16

POLYOMAVIRIDAE
Genome: dsDNA 1 CIRCULAR
Envelope:  NO
Morphology: ISOMETRIC
Virion Size (diameter [nM]): 40-45

Question 17

PAPILLOMAVIRIDAE
Genome: 
Envelope:  
Morphology:
Virion Size (diameter [nM]):

Answer 17

PAPILLOMAVIRIDAE
Genome: dsDNA 1 CIRCULAR
Envelope:  NO
Morphology: ISOMETRIC
Virion Size (diameter [nM]): 55

Question 18

HEPADNAVIRIDAE
Genome: 
Envelope:  
Morphology:
Virion Size (diameter [nM]):

Answer 18

HEPADNAVIRIDAE
Genome: dsDNA-RT 1 linear
Envelope:  +
Morphology: SPHERICAL
Virion Size (diameter [nM]): 42-50

Question 19

CIRCOVIRIDAE
Genome: 
Envelope:  
Morphology: 
Virion Size (diameter [nM]):

Answer 19

CIRCOVIRIDAE
Genome: SSDNA 1-or +/- CIRCULAR
Envelope:  NO
Morphology: ISOMETRIC
Virion Size (diameter [nM]): 12-27

Question 20

PARVOVIRIDAE
Genome: 
Envelope: 
Morphology:
Virion Size (diameter [nM]):

Answer 20

PARVOVIRIDAE
Genome: SSDNA 1 +/- LINEAR
Envelope:  NO
Morphology: ISOMETRIC
Virion Size (diameter [nM]): 18-26

Question 21

RETROVIRIDAE
Genome: 
Envelope:  
Morphology:
Virion Size (diameter [nM]):

Answer 21

RETROVIRIDAE
Genome: SSRNA-RT 1 + (DIMER)
Envelope:  +
Morphology: SPHERICAL
Virion Size (diameter [nM]): 80-100

Question 22

REOVIRIDAE
Genome: 
Envelope:  
Morphology: 
Virion Size (diameter [nM]):

Answer 22

REOVIRIDAE
Genome: dsRNA 10-12 segments
Envelope:  NO
Morphology: ISOMETRIC
Virion Size (diameter [nM]): 60-80

Question 23

BIRNAVIRIDAE
Genome: 
Envelope:  
Morphology:
Virion Size (diameter [nM]):

Answer 23

BIRNAVIRIDAE
Genome: dsRNA 2 segments
Envelope:  NO
Morphology: ISOMETRIC
Virion Size (diameter [nM]): 60

Question 24

PARAMYXOVIRIDAE
Genome: 
Envelope:  
Morphology:
Virion Size (diameter [nM]):

Answer 24

PARAMYXOVIRIDAE
Genome: NssRNA 1-segment
Envelope: +
Morphology: pleomorphic
Virion Size (diameter [nM]): ~150

Question 25

``` RHABDOVIRIDAE Genome: Envelope: Morphology: Virion Size (diameter [nM]): ```

Answer 25

``` RHABDOVIRIDAE Genome: NssRNA 1-segment Envelope: + Morphology: BULLET-SHAPED Virion Size (diameter [nM]): 100-430 X 45-100 ```

Question 26

``` FILOVIRIDAE Genome: Envelope: Morphology: Virion Size (diameter [nM]): ```

Answer 26

``` FILOVIRIDAE Genome: NssRNA, 1-segment Envelope: + Morphology: FILAMENTOUS Virion Size (diameter [nM]): 600-800 X 80in diameter ```

Question 27

``` BORNAVIRIDAE Genome: Envelope: Morphology: Virion Size (diameter [nM]): ```

Answer 27

``` BORNAVIRIDAE Genome: NssRNA 1-segment Envelope: + Morphology: SPHERICAL Virion Size (diameter [nM]): 80-100 ```

Question 28

``` ORTHOMYXOVIRIDAE Genome: Envelope: Morphology: Virion Size (diameter [nM]): ```

Answer 28

``` ORTHOMYXOVIRIDAE Genome: NSSRNA 6-8 - SEGMENTS Envelope: + Morphology: PLEOMORPHIC Virion Size (diameter [nM]): 80-120 ```

Question 29

``` BUNYAVIRIDAE Genome: Envelope: Morphology: Virion Size (diameter [nM]): ```

Answer 29

``` BUNYAVIRIDAE Genome: NSSRNA, 3- OR +/- SEGMENTS Envelope: + Morphology: SPHERICAL Virion Size (diameter [nM]): 80-120 ```

Question 30

``` ARENAVIRIDAE Genome: Envelope: Morphology: Virion Size (diameter [nM]): ```

Answer 30

``` ARENAVIRIDAE Genome: NSSRNA 2 +/- SEGMENTS Envelope: + Morphology: SPHERICAL Virion Size (diameter [nM]): 50-300 ```

Question 31

Which two viral families are ssRNA, have an envelope, spherical, 1 + segment, with sizes of 45-60 and 120-160?

Answer 31

Coronaviridae and Arteriviridae

Question 32

ssRNA, no envelope, isometric, 1+ segment, ~30nM | 3 families

Answer 32

Picornaviridae Caliciviridae Astroviridae

Question 33

Togaviridae and Flaviviridae--genome, envelope, morphology, size?

Answer 33

ssRNA, has envelope, spherical, 1+ segment, ~70 and 40-60