viral serology (hepatitis) Flashcards
resulta and interpretation
- HBsAg (+)
- Total anti-HBc (+)
- IgM anti-HBc (+)
- Anti-HBs (–)
acute hepa B
Serum Hepatitis is caused by the – HEPADNAVIRIDAE formerly known as the Australia or Hepatitis-associated Antigen
hepa B
- current infection
- used for viral load testing, blood/organ donor screening
- HCV phenotyping to determine optimal treatment
HCV RNA
- acute or chronic infection
- a defective virus that can only occur in presence of HBV
hepa D
- Appears during the recovery period of Acute Hepatitis B, weeks to months after HBsAg disappear.
- Provide Protective Immunity: ≥ 10 mIU/mL of Serum
anti-Hbs
- current infection
- marker of active viral replication
- used to monitor therapy
HDV RNA
results and interpretation
- HBs Ag (–)
- Total anti-HBc (+)
- Anti-HBs (+)
recovery from hepa B
- Result of Natural Infection or Immunization.
- Competitive Inhibition ELISA Test
IgG anti-Hav
- Present during active replication of the virus.
- Indicates high degree of infectivity.
HBeAg
- Marker of Acute Hepatitis A
- Peak: During First Mons. of Illness
- Declined: 6 to 12 Mons.
- Solid-Phase Antibody Capture ELISA
IgM anti-HAV
- transmission: sexual contact, blood
- incubation period: 60-90 days
- disease course: May be acute, chronic, or fulminant, or the patient may be a chronic asymptomatic carrier. Symptoms are similar to those seen in HAV infections
hepa B
Persists for the lifetime of the individual.
IgG anti-HBc
- transmission: fecal oral route
- incubation: 28 days
- disease course: acute and self limiting; no carrier state
- laboratory diagnosis: liver function test (ALT), total bilirubin, antibodies can be detected by EIA and RIA methods
hepa A
infectious hepatitis is caused by the HAV - PICORNAVIRIDAE
hepa A
1st Generation Test
ouchterlony
2nd Generation Tests
- CIE
- rheophoresis
- complement fixation
Marker of Convalescence.
anti-HBe
results and interpretation
- HBsAg (+)
- Total anti-HBc (+)
- IgM anti-HBc (–)
- Anti-HBs (–)
chronic hepa B/carrier
results and interpretation
- HBsAg (–)
- Anti-HBc (–)
- Anti-HBs (+)
hepa B immunization
resulta and interpretation
IgM anti-HAV (+)
acute hepa A
- Australian Antigen.
- First Marker to appear.
- Indicator of active infection
- Important marker in screening blood donor.
HBsAg
- Indicator of current or recent infection.
- Detects the “Core Window Period”
IgM anti-HBc
3rd Generation Tests (Most Sensitive)
- reverse passive latex agglutination
- reverse passive hemeagglutination
- ELISA
- RIA
- Not detected in serum.
- Detected through Liver Biopsy.
HBcAg
- acute, chronic, or previous infection
- Pos should be confirmed by recombinant immunoblot assay (RIBA) or molecular method
hepa C
resulta and interpretation
Total anti-HAV (+)
recovery from hepa A
