Viral Genomes and Diversiyy Flashcards
Obligate intracellular parasites
Virus
Complete, fully developed, infectious viral particle
Virion
Virion length
20 to 300 nanoneters
Nucleic acid surrounded by a protein coat
Capsid
Capsid composed of protein subunits
Capsomere
A unit of viral structure, consisting of a capsid with the enclosed nucleic acid
Nucleocapsid
Long rods that may be rigid or flexible
Helical Virus
Morphology, the capsule shape is icosahedron and capsomeres are equilateral triangle
Polyhedral virus
Rough speherical
Enveloped Virus
Polyhedral virus enclosed with an envelope
Enveloped polyhedral virus
Bacterial viruses have complicated structures
Complex viruses
Viral symmetry
Helical symmetry
Icosahedral
Complex
Rod shaped viruses (Tobacco Mosaic Virus)
Helical symmetry
Spherical shaped virus (Adenovirus, Herpes)
Icosahedral
Composed of several separate capsomere and symmetry (Poxvirus, Bacteriophage)
Complex
Viral genome
Consist of DNA or RNA
Linear or circular
Single or Double stranded
The single stranded genome has _____ and ______ sense
Positive and negative
Configuration that have the exact same base sequence as that of the viral mRNA to be translated to form viral proteins
Plus configuration
Complementary base sequence to viral mRNA
Minus configuration
Living characteristics of Viral Genome
They reporoduce but in host cells only
They can mutate
Non living characteristics of viruses
Acellular and contain no cytoplasm or organelles
Carry out no metabolism
Can be DNA or RNA
Viruses with DNA genome
Bacteriophage fX174
Bacteriophage M13
Contains circular genome of 5386 nucleotides inside a tiny icosahedral virion about 25nm in diameter
Phage fX174
Filamentous virus with helical symmetry, virion is long and thin and attaches to pilus of its host cell
Phage M13
Double stranded DNA bacteriophages
Phage 17
Phage Mu
Positive strand RNA viruses
Polio Virus
Coronavirus
Phage MS2
Negative strand RNA animal viruses
Rabies virus
Influenza Virus
T or F
There are negative strand RNA bacteriophages or archaeal viruses
F (no known)
Double stranded RNA Viruses
Reovirus
Rotavirus
Virus that use reverse transcriptase
Retrovirus
Hepadnavirus
A DNA virus enters host cell before it begins to replicate
Class 1 - dsDNA virus
Have circular genomes and replicate mostly within nucleus by a rolling circle mechanism
Class II ssDNA virus
Replicate in core capsid of the host cell cytoplasm
Class III: dsRNA virus
Have positive sense RNA genomes. They can be read by ribosomes and translate to proteins
Class IV ssRNA virus
Negative sense RNA genome, that must be transcribed by a viral polymerase in order to produce readable strand
Class V ssRNA virus
Positive sense, single stranded RNA genome but replicate through a DNA intermediate
Class VI Positive sense ssRNA reverse transcriptase virus
Have double stranded genome but replicate through ssRNA intermediate
Class VII dsDNA reverse transcriptase
Has been responsible in naming viruses
International committee on the Taxonomy of Viruses (ICTV)
ICTV names viruses based on
Nucleic acid type
Strategy for replication
Morphology
Order names ends in
Ales
Family names ends in
Viridae
Genus names ends in
Virus
A group of viruses sharing the same genetic information
Viral species
3 types of Bacterial identification
Western blotting
Restriction Fragment Length Polymorphisms (RFLP)
Polymerase chain reaction (PCR)
Viruses that infect bacteria
Bacteriophages
Bacteriophages that replicate through the lyctic life cycle. They lyse the host bacterium.
Virulent phages (Lytic cycle)
Do not immediately initiate lyctic cycle, rather becomes a non infectious prophage
Temperate phages (lysogenic cycle)
Used for detecting viruses
Plaque Assay Method
A process where the virus cannot replicate or reproduce unless the virion itself had gained entry into a suitable cell
Viral infection
Are infectious proteins
Prions
Infectious species of RNA, harmful to plants
Viroids
Difference of lyctic and lysogenic viral infection
Lyctic
DNA destroyed
Lysogenic
Merge with cell DNA
Lyctic
Produce progeny phages
Lysogenic
Not produce progeny phages
Virus lyctic cycle
Attachment
Penetration
Synthesis
Assembly
Release
Virus may replicate and destroy host
Virulent lytic infection
T or F
Viruses cause cancer
T