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P2: Bacteriology > Vibrio > Flashcards

Vibrio Flashcards

1
Q

Vibrio cholera strains produce Cholera toxin:

A

V. cholerae O1
V. cholerae O139 Bengal

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2
Q

V. cholerae O1 serotypes

A

Inaba
Ogawa
Hikojima

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3
Q

V. cholerae O1 biotypes:

A

a. Classical
b. El Tor

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4
Q

V. cholerae O1 serotypes:

antigentic determinant A & C

A

Inaba

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5
Q

V. cholerae O1 serotypes:

antigenic determinant A & B

A

Ogawa

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6
Q

V. cholerae O1 serotypes:

expresses both Inaba and Ogawa antigens

A

Hikojima

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7
Q

1 causative agent of cholera pandemic

A

Classical

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8
Q

Selective media:

A

Thiosulfate Bile Salt Sucrose (TCBS)
Alkaline Bile Salt Agar

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9
Q

Enrichment broth:

A

Alkaline Peptone Broth, pH 8.6

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10
Q

Vibrio spp can tolerate ____ pH

A

6.5-9.0

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11
Q

Motility

A

Darting Motility/Shooting Star Motility

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12
Q

If culture will be delayed, transport the spx in

A

Cary-Blair
Alkaline salt transport medium
VR
Autoclaved seawater

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13
Q

Pili of V. cholerae

A

toxin co-regulated pilus

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14
Q

have 2 chromosomes

A

V. cholerae and V. parahaemolyticus

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15
Q

Cell wall has lipopolysaccharide of 3 types:

A

a. Lipid A (endotoxin)
b. Core polysaccharide
c. O polysaccharide

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16
Q

used to subdivide Vibrio into serogroups

A

O polysaccharide

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17
Q

have acidic polysaccharides

A

V. vulnificus and Non-O1 V. cholerae

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18
Q

Virulence Factor of V. cholerae

A

Cholera toxin A & B (ctxA & ctxB)
Toxin co-regulated pilus (TCP)
Chemotaxis protein
Accessory cholera enterotoxin (ACE)
Zonula occludens toxins (ZOT)
Neuraminidase

19
Q

Virulence Factor of V. parahaemolyticus

A

Kanagawa hemolysin/ Thermostable direct hemolysin (TDH)

20
Q

Virulence Factor of V. vulnificus

A

Polysaccharide capsule
Cytolysins, proteases, collagenase
Lysine degradation enzymes

21
Q

VF Biological Effect:

Hypersecretion of electrolytes and water; similar to enterotoxion of E. coli

A

Cholera toxin A & B (ctxA & ctxB)

22
Q

VF Biological Effect:

Surface binding site receptor for bacteriophage CTXΦ; mediates bacterial adherence to intestinal mucosal cells

A

Toxin co-regulated pilus (TCP)

23
Q

VF Biological Effect:

Adhesin factor

A

Chemotaxis protein

24
Q

VF Biological Effect:

Increases intestinal fluid secretion

A

Accessory cholera enterotoxin (ACE)

25
Q

VF Biological Effect:

Increases intestinal permeability

A

Zonula occludens toxins (ZOT)

26
Q

VF Biological Effect:

Modifies cell surface to increase GM1 binding sites for cholera toxin

A

Neuraminidase

27
Q

VF Biological Effect:

Enterotoxin that induces chloride ion secretion (watery diarrhea); produces beta hemolysis on BAP using human blood

A

Kanagawa hemolysin/ Thermostable direct hemolysin (TDH)

28
Q

VF Biological Effect:

Antiphagocytic

A

Polysaccharide capsule

29
Q

VF Biological Effect:

Mediate tissue destruction

A

Cytolysins, proteases, collagenase

30
Q

VF Biological Effect:

Make the intestinal mucosa alkaline

A

Lysine degradation enzymes

31
Q

The only lactose fermenter

A

V. vulnificus

32
Q

Primary treatment for V. cholerae

A

fluid and electrolyte replacement

33
Q

Primary treatment for V. parahaemolyticus

A

fluid and electrolyte therapy

34
Q

Primary treatment for V. vulnificus

A

Antibiotic therapy

35
Q

is a milder form of diarrheal disease which occurs in toxin-negative strains of V. cholerae O1 and other non-O1 serotypes

A

Gastroenteritis

36
Q

fever is rare, maybe indicative of secondary infection

A

Vibrio cholerae O1 and O139

37
Q

1L of fluid is lost per hour, resulting into severe dehydration, muscle cramps, metabolic acidosis, hypokalemia and hypovolemic shock, cardiac arrhythmia and renal failure

A

Vibrio cholerae O1 and O139

38
Q

gastroenteritis, cholera-like illness

A

Vibrio parahaemolyticus

39
Q

disease begins at 5-72 hours after exposure with explosive watery diarrhea

A

Vibrio parahaemolyticus

40
Q

wound infection may also occur from exposure to contaminated seawater

A

Vibrio parahaemolyticus

41
Q

most common manifestation: (a) primary septicemia from ingestion of contaminated food or water or (b) rapidly progressive woundinfectionfromexposuretocontaminatedseawater

A

Vibrio vulnificus

42
Q

sudden onset of fever and chills, vomiting, diarrhea and abdominal pain, secondary skin lesions and tissue necrosis are often present

A

Vibrio vulnificus

43
Q

infection is common among pxs with hepatic disease, hematopoietic disease, chronic renal failure, & those who are receiving immunosuppressive drugs

A

Vibrio vulnificus

P2: Bacteriology (11 decks)

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