Enterobactenaceae / Opportunistic Pathogens

Question 1

also known as “FRIEDLANDER’S BACILLUS”

Answer 1

Klebsiella spp.

Question 2

General characteristics of klebsiella spp.

Gram stain
02 requirement
Motility on SIM
Describe Texture of Colonies
Quellung Test
TSI
Indole
MR/VP
Citrate
ODC
Urease

Answer 2

Gram stain: Gram negative
02 requirement: Facultative anaerobe
Motility on SIM: Negative
Describe Texture of Colonies: Mucoid/S colonies
Quellung Test: Positive, capsule
TSI: A/A, G+, Lactose fermenter
Indole: Negative
MR/VP: MR (-), VP (+)
Citrate: Positive
ODC: Negative
Urease: Positive

Question 3

ORNITHINE DECARBOXYLASE TEST on Klebsiella spp.

Medium:
CHO:
AA:
pH indicator:

Answer 3

Medium: Purple broth
CHO: Glucose
AA: Ornithine
pH indicator: Bromcresol purple (turns yellow in acid)

Question 4

ORNITHINE DECARBOXYLASE TEST Detects the ability of the bacteria to decarboxylate Ornithine as a source of Carbon to form the alkaline product, ______

Answer 4

Putrescine

Question 5

Turns the medium back to purple when ODC is released, from being yellow as a result of Glucose fermentation.

Answer 5

ORNITHINE DECARBOXYLASE TEST

Question 6

Positive and negative for Ornithine Decarboxylase (ODC)

Answer 6

+ Purple
- Yellow

Question 7

Detects the ability of the organism to produce urease which breaks down urea into ammonia and CO2.

Answer 7

Urease

Question 8

pH indicator of urease

Answer 8

Phenol red

Question 9

resembles Klebsiella spp., except that they are MOTILE & ODC (+)

Answer 9

Enterobacter spp.

Question 10

Enterobacter spp. examples

Answer 10

Enterobacter aerogenes
Enterobacter cloacae subsp. cloacae

Question 11

General Characteristics of Enterobacter spp.

Gram stain:_________________________________________
O2 requirement:_____________________________________ SIM:_______________________________________________ TSI:_______________________________________________
Presence of Capsule:__________________________________ IMVC:_____________________________________________ Urease:_____________________________________________ ODC:______________________________________________

Answer 11

Gram stain: Gram negative bacilli
O2 requirement: Facultative anaerobe
SIM: - - +
TSI: A/A, G+
Presence of Capsule: Present
IMVC: - - ++
Urease: +
ODC: +

Question 12

E. aerogenes VS E. cloacae

LOA

Answer 12

E. aerogenes: ++-
E. cloacae: -++

Question 13

the agent of “RED BREASTMILK”

Answer 13

Serratia spp.

Question 14

The red pigment produced by S. marcescens is also known as

Answer 14

2-methyl-3-amyl-6-methoxyprodigiosene.

Question 15

General Characteristics of Serratia spp.

TSI
ONPG test
Urease
DNAse test
Lipase
Gelatinase

Answer 15

TSI: K/A
ONPG test: Positive
Urease: Positive
DNAse test: Positive
Lipase: Positive
Gelatinase: Positive

Question 16

Serratia marcescens

SIM
IMVC

Answer 16

SIM: - -+
IMVC: - - ++

Question 17

a facultative anaerobe, H2S positive, verticaclly-transmitted bacteria

Answer 17

Citrobacter spp.

Question 18

General Characteristics of Citrobacter spp.

TSI:
ONPG:
ODC:

Answer 18

TSI: K/A, H2S+
ONPG: Positive
ODC: Positive

Question 19

Citrobacter freundii

SIM
IMVC

Answer 19

SIM: +_+
IMVC: -+-+

Question 20

The rapid urease, non-lactose fermenter group.

Answer 20

Proteus-Providencia-Morganella

Question 21

Proteus-Providencia-Morganella

Phenylalanine Deaminase
Motility
Methyl red
H2S

Answer 21

Proteus: ++++
Providencia: +++-
Morganella: +++-

Question 22

The “burnt chocolate” odor, and kidney stone-former.

Answer 22

Proteus spp.

Question 23

Proteus spp. examples

Answer 23

Proteus mirabilis
Proteus vulgaris

Question 24

P. vulgaris and P. mirabilis

LOA
Indole
Chloramphenicol
Ampicillin & Cephalosporins

Answer 24

P. vulgaris
LOA: -,-,-
Indole: Red, Positive
Chloramphenicol: R
Ampicillin & Cephalosporins: R

P. mirabilis
LOA: -+-
Indole: Yellow, Negative
Chloramphenicol: S
Ampicillin & Cephalosporins: S

Question 25

causative agent of Fowl cholera

Answer 25

Providencia spp.

Question 26

P. rettgeri VS P. stuartii

Urease: Pink - Mostly yellow
Ribitol/Adonitol fermentation: Acid is produced - Absence of acid
Trehalose fermentation: Absence of acid - Acid is produced

Answer 26

P. rettgeri
Urease: +
Ribitol/Adonitol fermentation: +
Trehalose fermentation: -

P. stuartii
Urease: Mostly -
Ribitol/Adonitol fermentation: -
Trehalose fermentation: +

Question 27

Morganella spp. examples

Answer 27

Morganella morganii subsp. morganii
M. morganii subsp. sibonii

Question 28

Providencia VS Morganella

H2S: No blackening - No blackening
Gas production: Intact TSI - Raised/Cracked TSI
Citrate: Blue - Green

Answer 28

Prividencia
H2S: -
Gas production: -
Citrate: +

Morganella
H2S: -
Gas production: +
Citrate: -