Vet Clin Path 2014 Flashcards
Guttural pouch lesion in a horse. Dx?
Dx: Fungal infection with probable spore formation, and marked neutrophilic inflammation
What is your diagnosis? Guttural pouch lesion in a horse. Vet Clin Pathol 43/4 (2014) 607–608.
Cytocentrifuged preparation of cerebrospinal fluid from sheep. Dx? Describe the structures.
Curved ellipsoid parasitic zoites (10.5–11.5 lm 9 4– 5 lm) are present in each image. They show granular basophilic cytoplasm with a clear area in one pole and an eosinophilic round nucleus in the other pole.
What is your diagnosis? Cerebrospinal fluid from a dog. Vet Clin Pathol 43/3 (2014) 467–468.
Histologic section of an intraocular mass from a dog. Dx? Describe. What is the stain in the bottom picture?
Dx: Iridociliary adenoma or ciliary body adenoma
Note the papillary pattern and the thick periodic acid Schiff positive basement membrane. (A) H&E (B) Periodic acid Schiff
What is your diagnosis? An intraocular mass in a dog. Vet Clin Pathol 43/2 (2014) 289–290.
Canine blood smear. Describe the changes.
Many of the platelets contain single to multiple, variably sized cytoplasmic vacuoles.
Platelet vacuoles in a dog with severe nonregenerative anemia: evidence of platelet autophagy
Adrenal tumor in a cat. Dx?
Dx: Aldosteronoma
Accuracy of cytology in distinguishing adrenocortical tumors from pheochromocytoma in companion animals. Vet Clin Pathol 43/3 (2014) 453–459.
Lesional skin biopsy from an African hedgehog with epitheliotropic T-cell lymphoma. What was it positive for?
CD3
Multicentric epitheliotropic T-cell lymphoma in an African hedgehog ( Atelerix albiventris ). Vet Clin Pathol 43/4 (2014) 601–604.
Histo of a pancreas in a dog. Describe. Dx?
Dx: bone within an islet cell carcinoma in the pancreas of a dog.
Monomorphic polygonal cells are interspersed by delicate fibrovascular stroma, typical of neuroendocrine packeting. Cells have moderate amounts of finely granular eosinophilic cytoplasm, basilar or centrally located round nuclei, clumped chromatin, and a single prominent nucleolus. Interspersed amongst the neoplastic cells are broad and irregularly arranged, anastomosing trabeculae of mineralized compact bone containing loosely set osteoblasts within lacunae.
Osseous metaplasia within a canine insulinoma. Vet Clin Pathol 43/1 (2014) 89–93.
Pancreatic mass in a dog. Dx? What is the special stain?
Dx: islet cell carcinoma in a dog.
Strong positive staining for insulin within neoplastic cells, consistent with a diagnosis of insulinoma.
Osseous metaplasia within a canine insulinoma. Vet Clin Pathol 43/1 (2014) 89–93.
Cytocentrifuged preparation of cerebrospinal fluid from sheep. Dx?
Dx: presence of zoites compatible with Sarcocystis sp
What is your diagnosis? Cerebrospinal fluid from a dog. Vet Clin Pathol 43/3 (2014) 467–468.
Histologic section of a dermal mass in a dog. Dx? Describe.
Dx: trabecular type trichoblastoma
The mass was predominantly comprised of spindled keratinocytes with elongated nuclei. Areas of peripheral palisading (arrows) help characterize this tumor as a trabecular type trichoblastoma.
What is your diagnosis? Dermal mass in a dog. Vet Clin Pathol 43/2 (2014) 285–286.
Smear of a plaque from a guttural pouch of a horse. Dx? What is the stain?
Methanolic Wright’s stain. Fungal infection with probable spore formation, and marked neutrophilic inflammation
What is your diagnosis? Guttural pouch lesion in a horse. Vet Clin Pathol 43/4 (2014) 607–608.
Histologic skin section from lesional skin in an African hedgehog. Dx? Describe.
Dx: epitheliotropic T-cell lymphoma.
(A) There are neoplastic lymphocytes arranged in large clusters in the epidermis
(B) The follicular epithelium is markedly distorted by large aggregates of neoplastic lymphocytes
(C) Sheets of neoplastic lymphocytes are present within the dermis and infiltrating the epithelium of the adjacent hair follicle (arrow)
Multicentric epitheliotropic T-cell lymphoma in an African hedgehog ( Atelerix albiventris ). Vet Clin Pathol 43/4 (2014) 601–604.
Cytologic images of strap-like cell from a rhabdomyosarcoma located around the lead wire of a pacemaker in a dog. Dx?
Rhabdomyosarcoma
Rhabdomyosarcoma associated with the lead wire of a pacemaker generator implant. Vet Clin Pathol 43/2 (2014) 276–280.
Mass in the tendon sheath of a dog. Dx? Describe.
Dx: benign giant cell tumor of the tendon sheath (GCTTS)
Cytologically, plump spindle cells exhibiting mild-to- moderate pleomorphism and large multinucleated cells with similar staining characteristics are arranged individually with round, uniform nuclei that are often arranged in botryose or rosette-like formations in the multi- nucleated cells. Cells from both populations have a moderate amount of pink product within their cytoplasm and are found amongst a considerable amount of pink, extracellular matrix or product.
Cytologic findings from a benign giant cell tumor of the tendon sheath in a dog. Vet Clin Pathol 43/2 (2014) 270–275.
Blood smear from a cat. Dx?
Dx: left shift with bacteremia
What is your diagnosis? Blood smear from a cat. Vet Clin Pathol 43/3 (2014) 465–466.
Adrenal tumor in a dog. Where in the adrenal is this from? Describe.
Cortex.
Cells show a low nuclear to cytoplasmic ratio, a large amount of basophilic cytoplasm that may contain small empty vacuoles, and uniform round to oval central to peripheral nuclei with coarse chromatin.
Accuracy of cytology in distinguishing adrenocortical tumors from pheochromocytoma in companion animals. Vet Clin Pathol 43/3 (2014) 453–459.
Bone marrow smear from a dog with a small mammary mass and disseminated intravascular coagulation. Dx?
Circulating and metastatic carcinomatous cells in BM.
What is your diagnosis? Abnormal cells on a blood smear from a dog. Vet Clin Pathol 43/3 (2014) 461–462.
Smear of a fine-needle aspirate from lesional skin in an African hedgehog. Describe. Dx?
Dx: epitheliotropic T-cell lymphoma.
There are variably sized lymphocytes with round or indented nuclei. Cytoplasm is scant to moderate and lightly to moderately basophilic. A few mitotic figures were found, and cytoplasmic fragments are easily identified in the background.
Multicentric epitheliotropic T-cell lymphoma in an African hedgehog ( Atelerix albiventris ). Vet Clin Pathol 43/4 (2014) 601–604.
Adrenal tumor in a dog. Describe. Where in the adrenal is this from? What is the pattern?
A group of cortical cells from an adrenal cortical tumor in a dog, showing a perivascular arrangement.
Accuracy of cytology in distinguishing adrenocortical tumors from pheochromocytoma in companion animals. Vet Clin Pathol 43/3 (2014) 453–459.
Histologic section of fibrosarcoma from a hamster. What are these cells positive for?
Vimentin.
Atypical fibrosarcoma in the skin of a Roborovski hamster (Phodopus roborovskii). Vet Clin Pathol 43/2 (2014) 281–284.
Blood smear from a dog with a small mammary mass and disseminated intravascular coagulation. Dx?
Circulating and metastatic carcinomatous cells in peripheral blood
What is your diagnosis? Abnormal cells on a blood smear from a dog. Vet Clin Pathol 43/3 (2014) 461–462.
Spleen of a cat. What are these structures? What stain are they positive for?
Gamna-Gandy bodies, Prussian Blue
Gamna-Gandy bodies: a case of mistaken identity in the spleen of a cat. Vet Clin Pathol 43/1 (2014) 94–100.
Blood smear from a 5-day-old anemic foal. (B) new methylene blue. Dx?
Dx: Inflammation and marked regenerative anemia in a foal with neonatal isoerythrolysis causing
What is your diagnosis? Blood smear from a foal. Vet Clin Pathol 43/2 (2014) 287–288.
Mass from the digit of a cat. Dx?
Dx: Metastatic carcinoma; mild histiocytic and neutr- ophilic inflammation; necrosis
What is your diagnosis? Aspirate from a digit in a cat. Vet Clin Pathol 43/2 (2014) 291–292
Scattergram from a Sysmex XT-2000iv showing forward scatter (size) on the y-axis and fluorescence (nucleic acid) on the x-axis of an anemic foal (A) and an age-matched nonanemic foal (B). What do the different areas represent? What did this foal have?
Dashed red line indicates RBCs, the solid red line indicates reticulocytes, and the solid blue line indicates platelets
Inflammation and marked regenerative anemia from NI
What is your diagnosis? Blood smear from a foal. Vet Clin Pathol 43/2 (2014) 287–288.
Fine-needle aspirate of a dermal mass located on the ear base of a dog. Dx?
Dx: Trabecular type trichoblastoma
What is your diagnosis? Dermal mass in a dog. Vet Clin Pathol 43/2 (2014) 285–286.
Fine-needle aspiration of popliteal lymph nodes from a Rhodesian Ridgeback dog. Dx?
Systemic lymphadenopathy and chorioretinitis due to protothecosis.
What is your diagnosis? Systemic lymphadenopathy and blindness in a dog from Italy. Vet Clin Pathol 43/4 (2014) 605–606.
Impression smear of an excised skin mass from a hamster. Dx?
Dx: fibrosarcoma (cyto dx was STS)
There is a small population of individually arranged oval to spindle-shaped cells in a background of blood, exhibiting a moderate degree of anisocytosis, coarsely stippled chromatin, and one or more prominent nucleoli.
Atypical fibrosarcoma in the skin of a Roborovski hamster (Phodopus roborovskii). Vet Clin Pathol 43/2 (2014) 281–284.
Fine-needle aspiration of vitreous humor from the right eye from a Rhodesian Ridgeback dog. Dx?
Dx: Systemic lymphadenopathy and chorioretinitis due to protothecosis
What is your diagnosis? Systemic lymphadenopathy and blindness in a dog from Italy. Vet Clin Pathol 43/4 (2014) 605–606.
Histologic appearance of a deep portion of a mass around a pacemaker in a dog. Dx? What are the arrows and asterisks pointing to?
Dx: Subcutaneous rhabdomyosarcoma (epidermis not present in section). Neoplastic cells (long arrow) sur- round 2 adjacent lead wire tracts (asterisks). There are multifocal extended regions of hemorrhage and tumor necrosis (short arrows).
Rhabdomyosarcoma associated with the lead wire of a pacemaker generator implant. Vet Clin Pathol 43/2 (2014) 276–280.
Urine sediment of a dog after pyometra surgery, on day of admission. Dx?
Dx: Cylinduria and fragments of renal tubules
What is your diagnosis? Urine sediment changes in a dog with hemorrhagic shock and disseminated intravascular coagulation after pyometra surgery. Vet Clin Pathol 43/3 (2014) 463–464.
Spleen from a cat. What are these?
Gamna-Gandy bodies
Gamna-Gandy bodies: a case of mistaken identity in the spleen of a cat. Vet Clin Pathol 43/1 (2014) 94–100.
Thymic mass in a dog. Dx? Describe. What is the stain in C? Describe the pattern.
Dx: thymoma
The mass consists of expansions of both well-differentiated thymic medulla (bottom right) that includes Hassall’s corpuscles, and thymic cortex (left, and upper) comprised of predominantly small lymphocytes with admixed paler staining epithelial cells.
(C) Immunohistochemistry of the thymoma. There is strong, localized, concentrated immunoreactivity with Pancytokeratin antibodies in the medulla (right side), and finer, more intercalated cytokeratin immunoreactivity in the cortex (left), typical of the thymus.
Thymoma-associated lymphocytosis in a dog. Vet Clin Pathol 43/4 (2014) 584–588.
Skin mass from a hamster. Dx? Describe. Describe the inset.
Dx: fibrosarcoma
(A) Spindle cells supported by collagenous stroma are invading and expanding in the dermis and subcutis.
(B) Neoplastic cells have a basophilic, vacuolated cytoplasm, and eccentric nuclei and appear like “ganglion-like” cells. There are occasional binucleate forms (asterisk). Inset: Higher magnification of “ganglion-like” cells.
Atypical fibrosarcoma in the skin of a Roborovski hamster (Phodopus roborovskii). Vet Clin Pathol 43/2 (2014) 281–284.
Urine sediment of a dog after pyometra surgery, on day of admission. Describe.
(A) There are 2 structures resembling fragments of renal tubules with retained cylindrical forms, linear arrays of uniform epithelial cells, and an intact lumen. There are also granular debris and fat droplets.
(B) Cylindrical structure resembling a fragment of a renal tubule with uniform epithelial cells and 3 erythrocytes.
What is your diagnosis? Urine sediment changes in a dog with hemorrhagic shock and disseminated intravascular coagulation after pyometra surgery. Vet Clin Pathol 43/3 (2014) 463–464.
Histologic section of the right limb of the pancreas in a dog. Dx? Describe.
Dx: bone within an islet cell carcinoma in the pancreas of a dog.
The parenchyma is disrupted by an expansile, partially encapsulated and mildly infiltrative, multilobulated mass that consists of densely arranged, monomorphic polygonal cells. Approximately 50% of the mass is com- posed of metaplastic, mature trabecular bone.
Osseous metaplasia within a canine insulinoma. Vet Clin Pathol 43/1 (2014) 89–93.
Fine-needle aspirate of an intraocular mass of a dog. (A) May Grunwald–Giemsa (B) Periodic acid Schiff. Dx?
Dx: Iridociliary adenoma or ciliary body adenoma
What is your diagnosis? An intraocular mass in a dog. Vet Clin Pathol 43/2 (2014) 289–290.
Adrenal tumor in a dog. Describe. Where in the adrenal is this from? What is the pattern?
A group of medullary cells from a canine pheochromocytoma showing a perivascular arrangement.
Accuracy of cytology in distinguishing adrenocortical tumors from pheochromocytoma in companion animals. Vet Clin Pathol 43/3 (2014) 453–459.
Fecal smear from a Macaca fascicularis presenting with liquid diarrhea, Lugol’s solution. Dx?
Dx: Blastocystis sp. infection
What is your diagnosis? Fecal smear stained with Lugol’s solution and Giemsa from a cynomolgus macaque (Macaca fascicularis) presenting with liquid diarrhea. Vet Clin Pathol 43/2 (2014) 293–294.
Mass around a pacemaker in a dog. Dx? What are the cells positive for?
Rhabdomyosarcoma
(A) Markedly pleomorphic round and elongate spindle cells are arranged in dense bundles within preexisting adipose tissue. There are multiple multinucleated cells, including strap-like cells (long arrows). There are multiple mitotic figures (short arrows). There is an individual necrotic cell (arrowhead).
(B) Neoplastic cells exhibit diffuse, strong, finely granular to globular cytoplasmic immunolabeling for a-sarcomeric actin.
Rhabdomyosarcoma associated with the lead wire of a pacemaker generator implant. Vet Clin Pathol 43/2 (2014) 276–280.
Popliteal lymph node from a Rhodesian ridgeback dog. Dx? Describe.
Dx: Systemic lymphadenopathy and chorioretinitis due to protothecosis
Round to oval organisms surrounded by thin unstained cell walls (black arrow). These structures had basophilic granular cytoplasm and contained variably sized endospores. Large granular lymphocytes (LGLs) are evident (red arrow).
What is your diagnosis? Systemic lymphadenopathy and blindness in a dog from Italy. Vet Clin Pathol 43/4 (2014) 605–606.
Fine-needle aspirate of a pancreatic mass in a dog. Dx?
Dx: carcinoma of neuroendocrine origin (primary differential diagnosis was an insulinoma)
Note a cluster of malignant epithelial cells with moderate amounts of pale basophilic cytoplasm with varying numbers of small punctuate vacuoles, and round nuclei with a fine chromatin structure and prominent nucleoli. There are also a few bare nuclei and ruptured cells, suggestive of neuroendocrine origin.
Osseous metaplasia within a canine insulinoma. Vet Clin Pathol 43/1 (2014) 89–93.
Mass in the tendon sheath of a dog. Dx? What is this tumor positive for?
Dx: Histopathologic sections of the metatarsal benign giant cell tumor of the tendon sheath (GCTTS)
(A) H&E, (B) vimentin, (C) cytokeratin AE1/3, and (D) S-100. Mononuclear and multinuclear synovioblastic cells have diffuse cytoplasmic immunopositivity for vimentin, multifocal cytoplasmic immunopositivity for cytokeratin AE1/3, and diffusely cytoplasmic and occasionally nuclear immunopositivity for S-100
Cytologic findings from a benign giant cell tumor of the tendon sheath in a dog. Vet Clin Pathol 43/2 (2014) 270–275.
