Vet Clin Path 2012 Flashcards
Imprint of ureteral mass from a dog. Dx?
Primary ureteral giant cell sarcoma
Note the pleomorphic multinucleated giant cells surrounded by transitional epithelial cells (A,C) or spindle-shaped cells (B).9100 objective. Wright–Giemsa.
Primary ureteral giant cell sarcoma in a Pomeranian. Vet Clin Pathol 41/1 (2012) 141–146.
Laryngeal rhabdomyoma in a dog. What is the tumor positive for? (+ controls in inset)
(A) Cells do not express pancytokeratin AE1/ AE3.
(B) Cells are strongly immunoreactive for myoglobin.
(C) Cells are strongly immunoreactive for muscle-specific actin.
(D) Most cells do not express vimentin; there are low numbers of weakly immunoreactive cells.
Laryngeal rhabdomyoma in a dog. Vet Clin Pathol 41/4 (2012) 590–593.
Rivalta test. Is this test + or -?
Positive Rivalta test. A precipitate forms on the surface of the acetic acid solution and slowly floats to the bottom of the reaction tube. (Left) After 1 second; (Center) after 3 seconds; (Right) after 7 seconds.
Diagnostic accuracy of the Rivalta test for feline infectious peritonitis. Vet Clin Pathol 41/4 (2012) 558–567.
Histo from ovarian mass from a dog. Dx? Describe growth patterns.
Dx: ovarian papillary carcinoma. (A) Note papilliform processes projecting to the surface of the ovary. (B) Note multi-branched papillae.
What is your diagnosis? Ascites fluid from a dog with abdominal distension. Vet Clin Pathol 41/4 (2012) 605–606.
Histologic sections of a subcutaneous mass near the anus of an intact female dog. Dx? Describe the staining patterns.
(A) Nests of neoplastic cells invade subcutaneous tissue and muscle (M).
(B) A few neoplastic cells form glandular structures containing eosinophilic secretions (arrowheads), and bundles of spindle cells are obvious. The border between the 2 components is indistinct.
(C) Glandular cells and secretions (arrowheads) are strongly positive for paradoxical concanavalin A staining. Spindle cells have positive cytoplasmic staining.
(D) Spindle cells and glandular epithelial cells are immunoreactive for cytokeratin (CAM 5.2).
(E) Tumor cells do not express vimentin, whereas blood vessels and inflammatory cells are immunoreactive.
(F) Tumor cells do not express a-smooth muscle actin; immunoreactive cells are vascular smooth muscle cells.
Cytologically atypical anal sac adenocarcinoma in a dog. Vet Clin Pathol 41/2 (2012) 291–294.
A-C Blood smear from a dog. Desribe. Dx?
D-E Bone marrow from same dog. Describe.
F- Histologic section same dog.
Intravascular lymphoma withe leukemia.
(A) Note the large neoplastic cells (larger than neutrophils in the field) with numerous distinct clear vacuoles.
(B) Occasionally, faint small azurophilic cytoplasmic granules (arrow) were noted, and scattered mitotic figures were present (arrow- head).
(C) Note neoplastic cell exhibiting phagocytosis of erythrocytes (arrow).
(D) Aspirate smears contained numerous hypercellular marrow particles, and neoplastic cells comprised 5–23% of nucleated cells (arrows). Inset: A phagocytic neoplastic cell (arrow).
(E) Neoplastic cells are similar to those in peripheral blood; note marked anisocytosis and anisokaryosis.
(F) Note neoplastic cells (arrow) similar to those in blood and bone marrow aspirates.
Canine intravascular lymphoma with overt leukemia. Vet Clin Pathol 41/1 (2012) 84–91.
Duodenal brush preparation from a dog. Differentials?
Etiologic differential diagnoses for zoites included Isospora spp., Hammondia spp., Toxoplasma gondii, and Neospora caninum.
Dx: Hammondia heydorni zoites with mild lymphocytic inflammation
What is your diagnosis? Duodenal brush preparation from a dog. Vet Clin Pathol 41/3 (2012) 431–432.
Thoracic fluid from a dog. Dx?
Lymphoplasmacytic B-cell lymphoma with IgM secretion (Waldenstrom’s macroglobulinemia) (cyto dx was lymphoma with plasmacytoid features)
What is your diagnosis? Pleural effusion in a dog with hypertension. Vet Clin Pathol 41/4 (2012) 599–600.
Fine-needle aspirate of a cutaneous mass on the dorsum of a dog. Describe the cell features. What was the dx? (made on histo)
(A) Note the presence of fine dark pigment consistent with melanin within the cytoplasm of the neoplastic cell in the center (arrow).
(B) Bizarre mitotic figures are present (arrow).
(C) Neoplastic cells are oval to fusiform with wispy cytoplasmic projections.
(D) Note the eosinophilic stippling condensing in globular aggregates of pink material in the paranuclear area of neoplastic cells.
Cytologic features of clear cell adnexal carcinoma in 3 dogs. Vet Clin Pathol 41/3 (2012) 405–411.
Laryngeal mass in a dog. Dx? Describe.
Dx: Laryngeal rhabdomyoma
Large polygonal eosinophilic cells arranged in solid sheets with abundant cytoplasm and a round often centralized nucleus
Laryngeal rhabdomyoma in a dog. Vet Clin Pathol 41/4 (2012) 590–593.
Fine-needle aspirate of a subcutaneous mass at the base of the external ear canal in a Sprague-Dawley rat. (A) Giemsa. (B) Papanicolau. Dx?
Zymbal gland adenoma
What is your diagnosis? Subcutaneous enlargement near the base of the ear canal in a Sprague-Dawley rat. Vet Clin Pathol 41/1 (2012) 160–161.
(Initial cyto differential diagnoses included epidermal inclusion cyst, basal cell tumor, Zymbal gland ductular hyperplasia, and Zymbal gland adenoma)
Fine-needle aspirate of a third eyelid mass in a Paint horse. Dx?
Dx: Epithelioid variant of hemangiosarcoma. (Cyto dx was malignant epithelial neoplasm, most likely anaplastic squamous cell carcinoma, with lymphoid hyperplasia or lymphocytic infiltration. Amelanotic melanoma was also considered)
What is your diagnosis? Fine-needle aspirate of a third eyelid mass in a Paint horse.Vet Clin Pathol 41/2 (2012) 299–300.
Histologic section of liver from a howler monkey. Dx? Describe the stages.
Dx: Toxoplasmosis
Note the large cyst containing several bradyzoites in the sinusoidal space.
What is your diagnosis? Hepatosplenomegaly in a howler monkey (Alouatta fusca). Vet Clin Pathol 41/3 (2012) 433–434.
Normal canine ovary. What stage of estrous is this? What are the cells present? Describe the histo pic.
Late diestrus
(A) Fine-needle aspirate of ovary with a network of capillaries (arrow) surrounded by numerous round to polyglonal luteal cells.
(B) Histologic section of a corpus luteum with marked vascularization (arrows).
Cytologic features of normal canine ovaries in different stages of estrus with histologic comparison. Vet Clin Pathol 41/3 (2012) 396–404.
Ovarian cyst fluid from a mare. What are the structures in the cyst fluid?
Ciliary tufts
(A) variably sized and stained ciliary tufts, (B) a ciliated columnar cell with a few ciliary tufts, and (C) ciliary tufts that are partially or completely ciliated.
What is your diagnosis? Equine ovarian cyst fluid. Vet Clin Pathol 41/3 (2012) 435–436.
Peripheral blood from a dog. Dx? Describe.
Acute myeloblastic leukemia. Note atypical mononuclear cells with high N:C ratios, basophilic cytoplasm, and round to indented nuclei with coarsely stippled chromatin and, occasionally, visible nucleoli. The cytoplasm occasionally contains pink cytoplasmic granules (arrow) or small distinct vacuoles (arrow, inset).
Acute myeloblastic leukemia with associated BCR-ABL translocation in a dog. Vet Clin Pathol 41/3 (2012) 362–368
Liver from a dog. Describe. What are the structures?
Hepatic parasitic cyst with a thick capsule (arrow) and an irregular inner surface.
What is your diagnosis? Peritoneal fluid from a dog with abdominal pain. Vet Clin Pathol 41/2 (2012) 297–298.
Histologic section of a heart from a lamb with myocarditis stained with polyclonal goat anti-cTnI antibody. Describe the staining patterns. What do these results indicate?
(A) Note negative immunoreactivity in affected myocytes (arrows) and positive immunoreactivity in unaffected myocytes. 10 objective.
(B) Note infiltrating macrophages with immunoreactivity for cTnI (arrows). 40 objective.
Acommercial human cTnI assay may be used to detect plasma cTnI concentrations in sheep, and cTnI may be used as a blood-based biomarker of myocarditis in this species.
Increased plasma cardiac troponin I concentration in lambs with myocarditis. Vet Clin Pathol 41/3 (2012) 375–381.
Impression smears of core biopsy samples of a subcutaneous mass near the anus of an intact female dog. Dx?
Dx: atypical anal sac adenocarcinoma (made on histo)
(A) Cells with elliptical nuclei are present in clusters. Some cells are radially arranged around small lumina that contain eosinophilic material (arrowheads).
(B) Neoplastic cells with round nuclei and pale to basophilic cytoplasm with indistinct borders form a cluster.
(C) Spindle cells have pale cytoplasm with indistinct borders, fine homogenous chromatin, and a small indistinct nucleolus or no nucleolus. Mild anisokaryosis and anisocytosis are noted.
(D) Some spindle cells are radially arranged.
Cytologically atypical anal sac adenocarcinoma in a dog. Vet Clin Pathol 41/2 (2012) 291–294.
Normal canine ovary. What stage of estrous is this? Describe the images.
Late diestrus
(B) Histologic section of a corpus luteum. Note increasingly vacuolated luteal cells and low numbers of neutrophils between luteal cells.
(D) Note many small vacuoles and luteal cells with finely vacuolated cytoplasm and round eccentric nuclei with stippled chromatin and sometimes a single nucleolus.
(F) Note intact leukocytes in the cytoplasm (emperipolesis) of luteal cells
Cytologic features of normal canine ovaries in different stages of estrus with histologic comparison. Vet Clin Pathol 41/3 (2012) 396–404.
Cytocentrifuged preparation of ovarian cyst fluid obtained for a mare postmortem. Dx?
Cyst fluid containing detached ciliary tufts
What is your diagnosis? Equine ovarian cyst fluid. Vet Clin Pathol 41/3 (2012) 435–436.
Blood film from a boa constrictor. Dx?
Inclusion body disease
What is your diagnosis? Blood film from a boa constrictor. Vet Clin Pathol 41/1 (2012) 158–159
Histo of a subcutaneous facial mass in a cow. Dx? Describe.
Dx: parotid adenocarcinoma
(A) Note area of neoplastic cell proliferation (left) adjacent to normal tissue (right). (B) Neoplastic cells arranged in islets surrounded by scant stroma.
Adenocarcinoma of the parotid salivary gland in a cow. Vet Clin Pathol 41/3 (2012) 424–428.
Histologic section of a subcutaneous nodule overlying the left epaxial musculature of a dog. Dx? Describe.
HSA
Vascular channels contain erythroid precursors and megakaryocytes.
What is your diagnosis? Cytologic findings from a subcutaneous nodule over the left epaxial musculature in a dog. Vet Clin Pathol 41/2 (2012) 295–296.
Blood from a goat. What are the structures the arrow is pointing to?
Ultrathin section of a large Heinz body (arrow) within an RBC ghost. The Heinz body is electron-dense, and dense aggregates of reticular material of similar electron density are associated with the plasma membrane; 3 platelets are evident.
Copper toxicosis in a Boer goat. Vet Clin Pathol 41/4 (2012) 502–508.
Histologic sections of cutaneous epitheliotropic lymphoma from a dog with a lip mass. What dual markers did the neoplasm express?
CD3 and c-kit
(A) Neoplastic cells, including cells infiltrating the mucosa, are immunoreactive for CD3 with a cytoplasmic to membranous staining pattern with a more intense membranous component. (B) Neoplastic cells are also immunoreactive for c-kit with a cytoplasmic to membranous staining pattern similar to that in A.
Cutaneous epitheliotropic lymphoma with dual CD3 and c-kit expression in a dog. Vet Clin Pathol 41/4 (2012) 594–598.
FNA from a normal canine ovary. What are the cells in this smear?
Blue-purple amorphous material is surrounded by loose aggregates of granulosa cells.
Cytologic features of normal canine ovaries in different stages of estrus with histologic comparison. Vet Clin Pathol 41/3 (2012) 396–404.
Histologic section of a mass at the base of the ear canal in a rat. H&E. Dx? Describe.
Dx: Zymbal gland adenoma
(A) The multilobulated mass has markedly dilated ducts lined by basophilic folds of epithelium. (B) Ducts are filled with laminated and amorphous keratin, and sometimes ghost cells are noted.
What is your diagnosis? Subcutaneous enlargement near the base of the ear canal in a Sprague-Dawley rat. Vet Clin Pathol 41/1 (2012) 160–161.
Histologic section of the right kidney in a dog. Dx?
Lymphoplasmacytic B-cell lymphoma with IgM secretion
Note effacement of normal tissue by neoplastic lymphocytes and plasma cells.
What is your diagnosis? Pleural effusion in a dog with hypertension. Vet Clin Pathol 41/4 (2012) 599–600
Histologic sections of duodenum from a dog. Describe. Dx? What are the special stains in C & D?
Histologic sections of duodenum from a dog with pyogranulomatous enteritis.
(A) Note a granuloma with many neutrophils, few macrophages, and low numbers of multinucleated giant cells. H&E,940 objective.
(B) Rare nonstaining hyphal structures with blunt rounded ends (arrow) are present. H&E, 100 objective.
(C) Silver staining highlights more hyphal structures. Gomori methenamine silver, 60 objective.
(D) Hyphae are immunoreactive for anti-Pythium insidiosum antibody. Avidin-biotin immunoperoxidase,60 objective.
Dual infection with Pythium insidiosum and Blastomyces dermatitidis in a dog. Vet Clin Pathol 41/3 (2012) 419–423
Fecal flotation (sugar solution) from a dog. What is this structure? Dx?
Small unsporulated oocyst
Dx: Hammondia heydorni infection
What is your diagnosis? Duodenal brush preparation from a dog. Vet Clin Pathol 41/3 (2012) 431–432.
Histologic section of spleen from a boa constrictor. Dx? Describe.
Dx: inclusion body disease.
Numerous lymphocytes in the white pulp contain eosinophilic intracytoplasmic inclusions (arrows). The inclusions are round to oval, measure 3–6 lm in diameter, and displace the nuclei to the periphery of the cells. H&E, bar = 20 lm.
Direct smear from the Sabouraud dextrose agar plate from corneal scraping from the ulcerative lesion from a horse. Dx?
Single-celled microconidia (arrows), multi-cellular macroconidia (arrowheads), and hyphae are present.
What is your diagnosis? Corneal scraping from an ulcerative lesion in a Quarter horse. Vet Clin Pathol 41/4 (2012) 601–602.
Direct smear of peritoneal fluid froma dog. Dx?
Echinococcosis
What is your diagnosis? Peritoneal fluid from a dog with abdominal pain. Vet Clin Pathol 41/2 (2012) 297–298.
Histologic section of a heart from a lamb. Dx?
Dx: myocarditis
Acute multifocal myocarditis is characterized by inflammatory infiltration and myocardial necrosis (N). H&E,40 objective.
Increased plasma cardiac troponin I concentration in lambs with myocarditis. Vet Clin Pathol 41/3 (2012) 375–381.
RBCs after exposure to recombinant canine interleukin-8 and zymosan-activated plasma compared with PBS (control). Describe the changes.
Membrane ruffling and pseudopodia (arrows)
Effect of recombinant canine interleukin-6 and interleukin-8 on tissue factor procoagulant activity in canine peripheral blood mononuclear cells and purified canine monocytes. Vet Clin Pathol 41/3 (2012) 325–335
Fine-needle aspirate of a gastric lymph node from a dog. Differentials?
Note parallel-walled, negative-staining hyphae consistent with Pythium insidiosum or Lagenidium spp.
Dual infection with Pythium insidiosum and Blastomyces dermatitidis in a dog. Vet Clin Pathol 41/3 (2012) 419–423
Immunohistochemical staining of histologic sections of the ureteral mass (giant cell sarcoma) from a dog. Describe the IHC patterns.
Wide-spectrum screening cytokeratin immunoreactivity was limited to transitional epithelium (A), and both the spindle-shaped cells and multinucleated giant cells were immunoreactive for vimentin (B), indicating a tumor of mesenchymal origin.
Low numbers of scattered histiocytic cells were immunoreactive for Mac 387 (C). S-100 highlighted both the spindle- shaped cells and multinucleated giant cells with nuclei staining with greater intensity (D).
Cells surrounding vessels were immuoreactive for generalized muscle actin (E), consistent with the presence of myofibroblasts. Only vascular endothelium was immunoreactive for von Willebrand factor (F).
Primary ureteral giant cell sarcoma in a Pomeranian. Vet Clin Pathol 41/1 (2012) 141–146.
Fine-needle aspirate of normal canine ovary. What are the cells present?
granulosa cells
(A) Note small round to elongated granulosa cells arranged in loose aggregates. (B) Note granulosa cells arranged radially around purple amorphous extracellular material
Cytologic features of normal canine ovaries in different stages of estrus with histologic comparison. Vet Clin Pathol 41/3 (2012) 396–404.
Fine-needle aspirate of a subcutaneous nodule overlying the left epaxial musculature of a dog. Dx?
Marked extramedullary hematopoiesis (EMH), predominantly erythroid, with evidence of acute and chronic hemorrhage
What is your diagnosis? Cytologic findings from a subcutaneous nodule over the left epaxial musculature in a dog. Vet Clin Pathol 41/2 (2012) 295–296.
(A) Histologic section of brain through lateral ventricle (asterisk). Describe changes. (B) GMS from same area.
(C) Histologic section of the right eye. Describe changes. (B) GMS from same area.
Dx?
(A) Note increased cellularity of the ependymal lining of the lateral ventricle (arrow). H&E. (B) Same section as (A); note the numerous GMS-positive Prototheca organisms in the ependymal lining of the lateral ventricle (asterisk). Gomori’s methenamine silver (GMS).
(C) The choroid is markedly expanded with a pocket of inflammation (arrow); the sclera (asterisk) provides orientation. H&E. (D) Same section as (C) showing increased magnification of the inflammatory pocket in the choroid; note numerous GMS-positive organisms.
Dx: protothecosis
Disseminated protothecosis diagnosed by evaluation of CSF in a dog. Vet Clin Pathol 41/1 (2012) 147–152
Figure 1. Blood smear from an ill cynomolgus macaque. Dx?
Inflammation and bacteremia
What is your diagnosis? CBC data and blood smear from a cynomolgus macaque (Macaca fascicularis). Vet Clin Pathol 41/1 (2012) 162–163.
Blood from a goat. A-D are subsequent days after presentation. Describe changes. Dx?
Copper toxicosis.
(A) Initial presentation; note rare macrocytic cells with baso- philic stippling.
(B) One day after presentation; note decreased RBC density, many Heinz bodies (inset), fusiform RBCs, and a megaloblastic RBC.
(C) Two days after presentation; note decreased RBC density, many Heinz bodies, fusiform RBCs, and ghost cells containing Heinz bodies. Inset: RBC with a Cabot ring.
(D) Three days after presentation; note decreased RBC density, many Heinz bodies, ghost cells containing Heinz bodies, and fusiform RBCs
Copper toxicosis in a Boer goat. Vet Clin Pathol 41/4 (2012) 502–508.
Histologic sections of a deep dermal mass from a cat. Dx? What is the mass + for?
Anaplastic sarcoma with giant cells
(A) Note numerous multinucleate giant cells and occasionally bizarre mitotic figures. (B) Note that neoplastic cells are strongly immunoreactive for vimentin. Nuclear Fast Red.
What is your diagnosis? Ventral neck mass in a cat. Vet Clin Pathol 41/4 (2012) 603–604
Histologic section of a ureteral mass from a dog. Dx? Describe.
Primary ureteral giant cell sarcoma.
Nests of transitional epithelium cover (A) and extend deeply into (B) the neoplastic popula- tion containing spindle-shaped cells and multinucleated giant cells.
Primary ureteral giant cell sarcoma in a Pomeranian. Vet Clin Pathol 41/1 (2012) 141–146.
Normal canine ovary. What stage of estrous is this? Describe the images.
Early diestrus
(A) Histologic section of a corpus luteum in early diestrus. Note luteal cells with abundant eosinophilic to amphophilic cytoplasm and, rarely, large intracytoplasmic vacuoles. H&E, 940 objective.
(C) Note low numbers of clear lipid vacuoles and variably sized luteal cells with abundant amphophilic to deeply basophilic cytoplasm, rare small vacuoles, and round nuclei with finely reticular chromatin and sometimes a single nucleolus; a binucleate cell is present.
(E) Note intact leukocytes in the cytoplasm (emperipolesis) of luteal cells.
Cytologic features of normal canine ovaries in different stages of estrus with histologic comparison. Vet Clin Pathol 41/3 (2012) 396–404.
Laryngeal mass in a dog. Dx?
Dx: Laryngeal rhabdomyoma
Laryngeal rhabdomyoma in a dog. Vet Clin Pathol 41/4 (2012) 590–593.
Fine-needle aspirate of a brain mass from a dog. Dx? Describe.
Primitive neuroectodermal tumor
Most of the cells are small to medium-sized round cells with scant cytoplasm. Note the numerous cytoplasmic fragments and disrupted cells in the background. Diff-Quik, bar = 20 lm.
Cytologic and immunohistochemical characterization of a primitive neuroectodermal tumor in the brain of a dog. Vet Clin Pathol 41/1 (2012) 153–157.
Histologic section of bone marrow from a dog. Dx? Describe.
Dx: acute myeloblastic leukemia.
Focal cellular areas, surrounded by hemorrhage, are composed of immature mononuclear cells and a few multinucleated cells. H&E,60 objective.
Acute myeloblastic leukemia with associated BCR-ABL translocation in a dog. Vet Clin Pathol 41/3 (2012) 362–368
Fine-needle aspirate of the right mandibular lymph node from a dog with a cutaneous lip mass. Dx?
Metastatic cutaneous epitheliotropic lymphoma (cyto dx was metastatic undifferentiated round cell tumor)
Note the atypical neoplastic round cell (inset).
Cutaneous epitheliotropic lymphoma with dual CD3 and c-kit expression in a dog. Vet Clin Pathol 41/4 (2012) 594–598.
Fine-needle aspirate of the spleen of a howler monkey. Dx?
Dx: Toxoplasmosis
What is your diagnosis? Hepatosplenomegaly in a howler monkey (Alouatta fusca). Vet Clin Pathol 41/3 (2012) 433–434.
Direct smear of ascites fluid a dog. Dx?
Ovarian papillary carcinoma (cyto ddx papilloma, well-differentiated carcinoma of undetermined origin, and mesothelioma)
What is your diagnosis? Ascites fluid from a dog with abdominal distension. Vet Clin Pathol 41/4 (2012) 605–606.
Blood smear from a white0tailed fawn. Dx?
Dx: Mycoplasma ovis-like organisms (arrow- heads)
white-tailed fawn with aspiration bronchopneumonia. Note the spherical, rod-shaped, and ring-shaped forms of the hemoplasma. In white-tailed deer, sickle-shaped RBCs (arrow) are a normal in vitro artifact. Modified Wright, bar = 10 lm.
Identification of a Mycoplasma ovis-like organism in a herd of farmed white-tailed deer (Odocoileus virginianus) in rural Indiana.Vet Clin Pathol 41/1 (2012) 77–83.
Corneal scraping from the ulcerative lesion from a horse. Dx?
Keratomycosis caused by complex Fusarium solani complex
What is your diagnosis? Corneal scraping from an ulcerative lesion in a Quarter horse. Vet Clin Pathol 41/4 (2012) 601–602.
Ovarian mass in a dog. Dx?
Ovarian carcinoma. Note the papillary growth pattern. H&E,10 objective.
Cytologic features and diagnostic accuracy of analysis of effusions for detection of ovarian carcinoma in dogs. Vet Clin Pathol 41/1 (2012) 127–132.
Peroxidase and basophil cytograms from (A,C) a control rat administered vehicle and (B,D) a rat administered a xenobiotic and euthanized on study day 2. What does each numbered section represent?
On peroxidase cytograms (A,B), region 1 contains neutrophils; 2, eosinophils; 3, clumped platelets; 4, nonspecific “noise,” platelets, and nucleated RBCs (nRBCs); 5, mostly lymphocytes with some basophils and nRBCs; 6, large unstained cells; 7, monocytes.
On basophil cytograms (C,D) region 1 contains leukocytes and nRBCs; 2, nonspecific “noise”; 3, lysis-resistant cells.
What is your diagnosis? CBC and cytogram data from a Sprague-Dawley rat. Vet Clin Pathol 41/1 (2012) 164–165.
Fine-needle aspirate of a subcutaneous facial mass in a cow. Dx? Describe.
Dx: parotid gland adenocarcinoma
(A) Cohesive sheets of cells with high nuclear:cytoplasmic ratios. (B) Large round to cuboidal large cells with mild anisocytosis and anisokaryosis.
Adenocarcinoma of the parotid salivary gland in a cow. Vet Clin Pathol 41/3 (2012) 424–428.
Histologic section of a punch biopsy specimen from a cutaneous lip mass in a dog. Dx? Describe.
Dx: Cutaneous epitheliotropic lymphoma
Note the monomorphic population of neoplastic round cells having plump round nuclei with cleaved and slightly pleomorphic forms.
Cutaneous epitheliotropic lymphoma with dual CD3 and c-kit expression in a dog. Vet Clin Pathol 41/4 (2012) 594–598.
Fine-needle aspirate of a mesenteric lymph node in a dog. Dx?
Lymphoplasmacytic B-cell lymphoma with IgM secretion (Waldenstrom’s macroglobulinemia) (cyto dx was lymphoma with plasmacytoid features)
What is your diagnosis? Pleural effusion in a dog with hypertension. Vet Clin Pathol 41/4 (2012) 599–600.
Immunohistochemical staining of a histologic section of a primitive neuroectodermal tumor in the brain of a dog. What are the neoplastic cells positive for?
(A) Neoplastic cells are variably immunoreactive for vimentin. Blood vessels within the tumor are strongly immunoreactive. Alkaline phosphatase, vector red chromogen.
(B) Neoplastic cells are immunoreactive for NSE, and many cells have strong positivity.
(C) Neoplastic cells are immunoreactive for S-100.
Cytologic and immunohistochemical characterization of a primitive neuroectodermal tumor in the brain of a dog. Vet Clin Pathol 41/1 (2012) 153–157.
Peroxidase and basophil cytograms from (A,C) a control rat administered vehicle and (B,D) a rat administered a xenobiotic and euthanized on study day 2. Describe the findings and what info was gleaned from this study.
Myelotoxicity was induced in vivo by the xenobiotic, result- ing in peripheral leukocyte pyknosis and karyorrhexis and analytical interference. Correspondingly,
The peroxidase cytogram (Figure 1) had multiple changes compared with the cytogram of a vehicle-control rat; changes consisted of decreased cells in the neutrophil and monocyte regions and poor separation between neutrophils and plate- lets and between platelets, lymphocytes, and the noise region. In the basophil cytogram, there were increased sig- nals in the region of increasing nuclear density where neu- trophils, eosinophils, and nRBCs tend to localize, and these signals were concentrated toward and into the region of non- specific noise. As the basophil cytogram is generated after the cytoplasm is stripped from the nuclei of most cells, these changes indicated the presence of significant numbers of nuclei or nuclear fragments of increased density in the sam- ple. Taken together with analyzer flags (not shown) that sig- nified increased noise, discordance between WBC counts in the peroxidase and basophil channels, and clumping of plate- lets, these findings indicated that WBC and leukocyte differ- ential counts generated by the ADVIA were unreliable for this animal. Evaluation of the blood smears revealed large numbers of pyknotic and karyorrhectic cells and pyknotic cellular debris (Figure 2).
What is your diagnosis? CBC and cytogram data from a Sprague-Dawley rat. Vet Clin Pathol 41/1 (2012) 164–165.
Conjunctival epis from feline cornea. Dx?
Dx: Chlamydial inclusions (which were often located near the nucleus in cats positive for Chlamydophila felis by PCR).
Evaluation of cytologic findings in feline conjunctivitis. Vet Clin Pathol 41/2 (2012) 283–290.
Histologic section of liver from a howler monkey. Dx? What is the stain?
Dx: Toxoplasmosis
A tissue cyst is immunoreactive for Toxoplasma antigen. 3,3′-Diaminobenzidine
What is your diagnosis? Hepatosplenomegaly in a howler monkey (Alouatta fusca). Vet Clin Pathol 41/3 (2012) 433–434.
Histologic section of duodenum from a dog. Dx?
Dx: Hammondia heydorni zoites
What is your diagnosis? Duodenal brush preparation from a dog. Vet Clin Pathol 41/3 (2012) 431–432.
Bone marrow aspirate from a dog. Dx? Describe.
Dx: acute myeloblastic leukemia
Mononuclear cells predominate and have high N:C ratios and basophilic cytoplasm. Some cells contain discrete vacuoles or coarse pink granules (arrow). A large multinucleated cell (arrowhead) is present and has a high N:C ratio, basophilic cytoplasm containing small vacuoles, and 8 round to oval nuclei. Modified Wright–Giemsa,100 objective
Acute myeloblastic leukemia with associated BCR-ABL translocation in a dog. Vet Clin Pathol 41/3 (2012) 362–368
Cutaneous mass in a dog. Describe the histo. Dx?
Histiocytic sarcoma
(A) Nonencapsulated, poorly circumscribed dermal masses are composed of round to spindle-shaped cells that extend into the subcutis, often effacing or displacing adnexal structures; densely packed sheets of cells are arranged in multiple coalescing nodules that sometimes surround a central area of necrosis. 2 objective. (B) Note densely packed sheets of round to spindle-shaped cells. Bar = 20 lm
Multifocal cutaneous histiocytic sarcoma in a young dog and review of histiocytic cell immunophenotyping. Vet Clin Pathol 41/3 (2012) 412–418.
Histologic sections of a third eyelid mass in a Paint horse. Dx? Describe. What is the inset stain?
Dx: Epithelioid variant of hemangiosarcoma
(A) Note poorly formed vascular channels containing few RBCs and variable numbers of lymphocytes. Inset: Well-differentiated area with variably sized and shaped vascular spaces filled with RBCs. H&E.
(B) Epithelioid neoplastic cells have marked anisokaryosis; a mitotic figure (arrow) is present. H&E. Inset: Neoplastic cells are immunoreactive for von Willebrand factor. Immonoperoxidase/3,-3’-diaminobenzidine/Mayer’s hematoxylin.
What is your diagnosis? Fine-needle aspirate of a third eyelid mass in a Paint horse.Vet Clin Pathol 41/2 (2012) 299–300.
Normal canine ovary. What are the pink structures from? What stage of estrous does this suggest?
Corpus albicans, late diestrus
Other findings: few luteal cells and round cells
Cytologic features of normal canine ovaries in different stages of estrus with histologic comparison. Vet Clin Pathol 41/3 (2012) 396–404.
Peritoneal fluid from 2 dogs. May-Grunwald Giemsa. Dx? Describe.
Dx: ovarian carcinoma. (A) Numerous large papillary aggregates of cells are present in a background of blood. 910 objective. (B) A papillary aggregate of cells with an even border and acinar arrangement are present. Neoplastic cells are monomorphic and show mild cytologic atypia. 40 objective.
Cytologic features and diagnostic accuracy of analysis of effusions for detection of ovarian carcinoma in dogs. Vet Clin Pathol 41/1 (2012) 127–132.
Fine-needle aspirate of a deep dermal mass located on the ventral neck of a cat. Dx?
Anaplastic sarcoma with giant cells (The main cytologic differential diagnosis was sarcoma, including anaplastic sarcoma with giant cells, histiocytic sarcoma, or sarcoma of other origin, such as muscle)
What is your diagnosis? Ventral neck mass in a cat. Vet Clin Pathol 41/4 (2012) 603–604
Histologic sections of cerebrum (A) and heart (B) of a dog.
C&D- What were these cells positive for? What did that suggest the lineage was?
What was the dx?
(A) Neoplastic cells, similar to those in blood and bone marrow, fill a cerebral vessel.
(B) Small discrete right atrial mass with sheets of neoplastic cells (asterisk) adjacent to normal cardiac muscle (arrow).
(C,D) Granzyme B immunoreactivity of neoplastic cells in vessels in the cerebrum (C) and the cardiac mass (D). Based on other immunophenotyic findings, strong expression of granzyme B was most consistent with a neoplasm of NK cell origin.
Dx: Intravascular lymphoma withe leukemia.
Canine intravascular lymphoma with overt leukemia. Vet Clin Pathol 41/1 (2012) 84–91.
Normal canine ovary. What is the arrow pointing at?
Corpus albicans. Note the irregular connective tissue (arrow) persisting from a degenerated corpus luteum.
Cytologic features of normal canine ovaries in different stages of estrus with histologic comparison. Vet Clin Pathol 41/3 (2012) 396–404.
Histologic section of a brain mass from a dog. Dx? Describe.
The neoplasm with sheets of small round cells with hyperchromatic nuclei. There is multifocal palisading around vessels forming pseudorosettes. Distinct rosettes are not present.
Cytologic and immunohistochemical characterization of a primitive neuroectodermal tumor in the brain of a dog. Vet Clin Pathol 41/1 (2012) 153–157.
Conjunctival epis from feline cornea. Dx?
Dx: Mycoplasma organisms in a PCR-positive cat (paler than chlamydial inclusions and were often near the edge of the epithelial cells)
Evaluation of cytologic findings in feline conjunctivitis. Vet Clin Pathol 41/2 (2012) 283–290.
Fine-needle aspirate of a cutaneous nodule under the right eye of a dog. Describe the cell features. What was the dx? (made on histo)
(A) Note cells arranged individually or in loose clusters.
(B) Neoplastic cells display multinucleation, marked anisokaryosis, and prominent and sometimes large nucleoli.
(C) Neoplastic cells have indistinct cytoplasmic borders. The light blue to gray background is the result streaming of cytoplasm from ruptured cells.
(D) Few neoplastic cells contain fibrillar to needle-shaped bright pink material (arrow).
Cytologic features of clear cell adnexal carcinoma in 3 dogs. Vet Clin Pathol 41/3 (2012) 405–411.
Cytocentrifuged preparation of cerebrospinal fluid from a dog. Dx? Describe. What are arrows pointing to? What is the stain in D?
Dx: protothecosis
(A) Marked eosinophilic pleocytosis; note the large granu- lar lymphocyte with numerous azurophilic granules (arrow).
(B) Several oval to kidney-bean shaped basophilic and granular Prototheca organisms with thin non-staining casings (arrows).
(C) Empty casings (theca) in the cytoplasm of a large mononuclear cell (arrows).
(D) Numerous GMS-positive organisms.
Disseminated protothecosis diagnosed by evaluation of CSF in a dog. Vet Clin Pathol 41/1 (2012) 147–152
Peripheral blood from a reindeer. Dx?
Theileria
Note the small (1–2 lmin length) signet ring (short arrow) and rod-shaped (long arrow) piroplasms with an eccentric magenta nucleus and basophilic cytoplasm in 2 RBCs. Wright–Giemsa
Theileriosis in a reindeer (Rangifer tarandus tarandus) associated with a potentially novel Theileria sp. Vet Clin Pathol 41/4 (2012) 497–501.
Histologic section of a parotid adenocarcinoma in a cow. What were these cells + for?
p63
Adenocarcinoma of the parotid salivary gland in a cow. Vet Clin Pathol 41/3 (2012) 424–428.
Cutaneous masses in 3 dogs. What was the dx? What 2 IHC markers were these tumors + for?
(A) Dermal well-demarcated, multinodular proliferation of neoplastic cells (Dog 1). A focus of mineralization is present within the neoplasm (arrow).
(B) Nests of neoplastic cells with extensive cytoplasmic clearing (Dog 1).
(C) Lobules of neoplastic epithelial cells supported by fibrous stroma (Dog 3).
(D) Neoplastic cells with variable cytoplasmic clearing. Note extensive pleomorphism of neoplastic cells in some regions (Dog 3).
(E,F) Neoplastic cells with positive cytoplasmic immunoreactivity for (E) pancytokeratin and (F) vimentin
Cytologic features of clear cell adnexal carcinoma in 3 dogs. Vet Clin Pathol 41/3 (2012) 405–411.
