Vet Clin Path 2011 Flashcards
Typical flow cytometric results for erythrocytes from DEA 1.1-negative (A–C) and DEA 1.1-positive (D–F) dogs after incubating erythrocytes with anti-DEA 1.1 serum. Describe.
Forward scatter (FSC-H) and side scatter (SSC-H) were used to gate erythrocytes and separate them from contaminating platelets (A, D).
In the DEA 1.1-positive samples (D), there was a characteristic second small population of gated cells with greater forward scatter, probably erythrocyte aggregates. Scatterplots of fluorescence intensity (FL1-H) vs forward scatter of 10,000 gated events (erythrocytes) showed increased fluorescence of DEA 1.1-positive erythrocytes with a characteristic reverse comma scatter (E). Frequency distribution plots (C, F) of fluorescence intensity showed a clear difference in fluorescence between DEA 1.1-negative (C) and DEA 1.1-positive (F) dogs. All scatter and fluorescence axes are log scales.
Flow cytometric assessment of canine erythrocytes and platelets for dog erythrocyte antigen 1.1. Vet Clin Pathol 40/4 (2011) 435–443
Fine-needle aspirate of an ulcerative skin lesion in a dog. Wright–Giemsa, 100 objective. Differentials?
The differentials for the protozoal organisms included Neospora caninum, Toxoplasma gondii,and Sarcocystis spp.
Final dx was cutaneous neosporosis with suppurative inflammation.
What is your diagnosis? Fine-needle aspirate of ulcerative skin lesions in a dog. Vet Clin Pathol 40/3 (2011) 401–402
Imprint of a prescapular lymph node from a cow with severe anemia and leukopenia. Dx?
Many trypomastigotes of Trypanosoma vivax are found among the RBCs and lymphocytes. Quick Romanowsky stain. Inset: Trypomastigote with centrally placed vesicular nucleus and sub- terminal kinetoplast.
What is your diagnosis? Lymphadenopathy in a cow with severe anemia. Vet Clin Pathol 40/1 (2011) 103–104.
Typical flow cytometric scatterplots for control platelets (A–D) and platelets from DEA 1.1-negative (E) and DEA 1.1-positive (F) dogs. Describe the results.
Forward scatter (FSC-H) and side scatter (SSC-H) were used to gate washed platelets (A) and exclude microparticles and rare contaminating erythrocytes. Fluores- cence was recorded for 10,000 gated events (platelets). Platelet incubation with submaximal concentrations of positive control canine plasma contain- ing antibodies to platelet glycoprotein Ib/IX consistently generated strong fluorescence (FITC anti-IgG) for platelet surface-associated IgG (B). Incubation of platelets with buffer (C) consistently yielded negligible autofluorescence, and there was little increase in fluorescence when platelets were incubated with only the FITC-conjugated anti-[canine IgG] (D). There was no appreciable difference between the fluorescence of platelets from DEA 1.1-negative dogs (E) and DEA 1.1-positive dogs (F) after incubation with anti-DEA 1.1 serum.
Flow cytometric assessment of canine erythrocytes and platelets for dog erythrocyte antigen 1.1. Vet Clin Pathol 40/4 (2011) 435–443
Histologic section of the digestive system of a mussel. Dx? Describe the stages and structures in each image.
Dx. Martiellosis (Marteilia refringens)
H&E.
(A) Early phase: M. refringens primary cells (a) in gut epithelial cells.
(B) Mature phase: sporangial structures with up to 8 internal spores (b); interstitial hemocytes, consistent with moderate inflammation; arrowhead, digestive cells with cytoplasmic vacuoles. Inset: Refringent bodies in the cytoplasm of a sporangium (arrow).
What is your diagnosis? Pale yellowish digestive gland and watery tissues in Mediterranean mussels. 274 Vet Clin Pathol 40/2 (2011) 273–274
Bone marrow aspirate from a hyacinth macaw. Describe. Dx?
Dx: leukemia of unknown lineage
Many blast cells and mitotic figures. Wright–Giemsa,100 objective.
What is your diagnosis? Bone marrow aspirate from a hyacinth macaw (Anodorhynchus hyacinthinus). Vet Clin Pathol 40/4 (2011) 565–566
Cytocentrifuged preparation of cerebrospinal fluid from a dog. Dx?
Eosinophilic pleocytosis due to protothecosis
What is your diagnosis? Cerebrospinal fluid from a dog. Vet Clin Pathol 40/1 (2011) 105–106
Histologic section of a anal sac gland carcinoma located in the perianal region in a cat. Describe. Dx? What is the stain in the bottom picture?
Anal sac carcioma
(A) Note the neoplastic cells arranged in papillae and palisades, the desmoplastic reaction, distinct single nucleoli, and a few mitotic figures. H&E, 9 20 objective.
(B) Note partial strong immuno- reactivity of neoplastic epithelial cells for CAM 5.2. H&E counterstain.
What is your diagnosis? Intracranial mass in a cat. Vet Clin Pathol 43/4 (2014) 611–612.
Fine-needle aspirate of a subconjunctival mass in the left eye of a horse. ID.
Mycetoma caused by Scedosporium apiospermum
(A) Fungal hyphae with thick nonstaining cell walls (arrow). RBCs, cellular debris, degenerate neutrophils, and macrophages are present in the background. (B) Marked neutrophilic inflammation (arrow- head) and globular yeast-like fungal structures (arrow). Wright–Giemsa.
Subconjunctival mycetoma caused by Scedosporium apiospermum infection in a horse. Vet Clin Pathol 40/1 (2011) 84–88
Sedimented urine froman adultmale intact mixed-breed dog with hematuria. Dx?
Dx: Dioctophyma renale infection
What is your diagnosis? Unexpected finding in sedimented urine from a dog. Vet Clin Pathol 40/3 (2011) 403–404.
Densitometer tracings obtained from agarose gel electrophoresis of (A) serum and (B) urine from a dog. Describe. Dx?
Waldenstrom’s macroglobulinemia.
(A) Note the narrow peak in the b-globulin fraction, suggestive of the presence of a monoclonal paraprotein (albumin 35.1 g/ L, a1 4.7 g/L, a2 12.9 g/L, and b2 64.3 g/L). (B) Note the small peak in a similar region (total protein 6.5 g/L, albumin 2.3 g/L, b-fraction 0.9 g/L). Marked albuminuria is present, suggestive of glomerulopathy
Waldenström’s macroglobulinemia in a dog with a bleeding diathesis. Vet Clin Pathol 40/3 (2011) 351–355
Transmission electron micrograph of a neutrophil in the blood of a dog. Describe. Dx?
May–Hegglin anomaly
Note the oval cytoplasmic inclusion (arrowheads) that is devoid of neutrophil granules and does not appear to be membrane-bound. Parallel thin filaments and ribosomes are present.
May–Hegglin anomaly in a dog. Vet Clin Pathol 40/2 (2011) 207–214.
Peritoneal fluid from a horse. Descibe. What is the material? What happened in this horse? What is the stain on the bottom?
(A) Intracellular round, clear, nonrefractile globular material is present within neutrophils and macrophages. Diff-Quik. (B) The extracellular and intracellular glob- ular material stains pink to bright red. Oil Red O.
presumed leakage of mineral oil through a transmural rectal perforation
Oil Red O-positive lipid in peritoneal fluid from a horse with a rectal tear. Vet Clin Pathol 40/2 (2011) 265–269
Histologic section of brain in the region of the hippocampus of a dog. Describe. Dx? What is the stain the bottom image?
(A) Note multifocal perivascular cuffs composed of aggregates of numerous lymphocytes, plasma cells, and small numbers of histiocytes. H&E. Inset: Inflammatory cells in a perivascular cuff. H&E, bar = 20 mm. (B) Note algal organisms with characteristic radially arranged multiple wedge-shaped endospores. Gomori’s methanamine silver
What is your diagnosis? Cerebrospinal fluid from a dog. Vet Clin Pathol 40/1 (2011) 105–106
Histologic section of a lingual liposarcoma in a dog. What 2 stains is this tumor positive for?
Histologic section of a lingual liposarcoma in a dog.
A) S100 protein and (B) vimentin.
Cytologic, histologic, and immunohistochemical features of lingual liposarcoma in a dog. Vet Clin Pathol 40/3 (2011) 393–397.
Histologic section of spleen from a dog. Dx? Describe.
Dx: acute megakaryoblastic leukemia. The red pulp is diffusely effaced by sheets of mononuclear round cells and low numbers of dysplastic megakaryocytes with dispersed nuclei (arrows). H&E, 920 objective; inset, 40 objective.
Myeloperoxidase-positive acute megakaryoblastic leukemia in a dog. Vet Clin Pathol 40/4 (2011) 530–537
Histologic section of a renal mass from a dog. Describe. Dx?
Dx: renal carcinoma
(A) tubular to cribriform-like pattern, necrosis, and occasionally infiltrating neutrophils. (B) Neoplastic cells with variably vacuolated and eosinophilic cytoplasm, pleomorphic nuclei, and frequent mitotic figures
Paraneoplastic leukocytosis in a dog with a renal carcinoma. Vet Clin Pathol 40/1 (2011) 89–94.
Impression smear of lingual mass in a dog. Dx?
Liposarcoma (The cytologic diagnosis was sarcoma, and differential diagnoses included fibrosarcoma, liposarcoma, hemangiosarcoma, rhabdomyosarcoma, melanoma, or other soft tissue sarcoma)
What is your diagnosis? Lingual mass in a dog. Vet Clin Pathol 40/4 (2011) 561–562.
Clot retraction in blood samples from a Peruvian Paso mare and a healthy control horse. Describe the results.
Clot retraction was normal in the control sample, but no retraction was evident in the sample from the mare after several hours, supporting abnormal platelet function.
Glanzmann thrombasthenia in a 17-year-old Peruvian Paso mare. Vet Clin Pathol 40/1 (2011) 48–51
Blood smear from hyacinth macaw. Describe.
A few folded and dacryocyte-like erythrocytes. Wright–Giemsa, 100 objective.
What is your diagnosis? Bone marrow aspirate from a hyacinth macaw (Anodorhynchus hyacinthinus). Vet Clin Pathol 40/4 (2011) 565–566
Histology of an ovarian mass in a mare. Dx? Describe structures in the inset.
Dx: granulosa-theca cell tumor
Note aggregates of neoplastic granulosa cells surrounded by eosinophilic stroma. H&E. Inset: Call-Exner body surrounded by neoplastic granulosa cells. 40 objective.
What is your diagnosis? Unilateral ovarian mass in a mare. Vet Clin Pathol 40/3 (2011) 399–400
Impression smear of an ovarian mass in a mare. Dx?
Dx: granulosa-theca cell tumor
A longitudinal groove, with dense chromatin in the shape of an ‘‘H,’’ is present in a nucleus (arrow). Giemsa
What is your diagnosis? Unilateral ovarian mass in a mare. Vet Clin Pathol 40/3 (2011) 399–400
ID the structures
Blood film from a dog with Babesia canis rossi infection. Protozoal piroplasms within RBCs vary from round (A) to piriform (B) and are sometimes present in large numbers (C). An occasional ghost RBC (D, arrows) contains a piroplasm. Protozoal merozoites are also present extracellularly (E, F). Aqueous Romanowsky stain, bar = 10 mm.
Babesia canis rossi infection in a Texas dog; Vet Clin Pathol 40/3 (2011) 345–350
Fine-needle aspirate of a cutaneous mass in the mammary region of an intact female mixed breed dog. Giemsa. Dx? What is this stain?
Acantholytic squamous cell carcinoma (ASCC)
The cytoplasm of the cells is orangeophilic, indicating keratinization and squamous differentiation. Papanicolaou
What is your diagnosis? Cutaneous mass in the mammary region of a dog. Vet Clin Pathol 40/1 (2011) 101–102
Histologic sections of a subconjunctival mass in the left eye of a horse. ID/Describe. What is the stain on the right?
(A) Note rim of neutrophils (black arrowhead) surrounding the fungal elements (arrow) and occasional multinucleated cells (gray arrowhead). H&E. (B) Note black-stained fungal structures with dilated hyphae (arrow). Gomori methenamine silver.
Subconjunctival mycetoma caused by Scedosporium apiospermum infection in a horse. Vet Clin Pathol 40/1 (2011) 84–88
Histo of ulcerative an ulcerative skin lesion in a dog. Dx? Decribe structure in inset.
Histologic section of skin with focally severe chronic purulent dermatitis and panniculitis with intralesional protozoal cysts and protozoal
organisms phagocytosed by macrophages. H&E, 50 objective. Inset: Protozoal organisms phagocytosed by amacrophage. 100 objective.
What is your diagnosis? Fine-needle aspirate of ulcerative skin lesions in a dog. Vet Clin Pathol 40/3 (2011) 401–402
Imprint of the digestive gland of a mussel. Diagnosis? Describe.
Dx: Marteiliosis (Marteilia refringens)
In addition to cells of the digestive gland (Figure 1, large arrowhead) and inflammatory hemocytes (Figure 1, small arrowhead), cytologic preparations contained numerous protozoal organisms; some were 4mm and others 30–40mm in diameter (Figure 1, a and b). They resembled primary cells (nurse cells) of paramyxean parasites and were characterized by basophilic cytoplasm and 1 or more eosinophilic nuclei surrounded by pale halos. There were also polynucleated (sporulated) cells containing spores and refringent bodies (RB) typical of Marteilia spp. (Figure 1, arrow).
What is your diagnosis? Pale yellowish digestive gland and watery tissues in Mediterranean mussels. 274 Vet Clin Pathol 40/2 (2011) 273–274
Imprint of a cerebral mass in a dog. Dx?
Intracranial cavernous hemangioma (cyto dx was mesenchymal neoplasm, with meningioma or well-differentiated hemangiosarcoma as the differential diag- nosis, with evidence of previous hemorrhage).
What is your diagnosis? Intracranial mass in a dog. Vet Clin Pathol 40/4 (2011) 563–564.
Histologic section of a lingual mass in a dog. Describe. Dx?
Dx: liposarcoma (The histopathologic diagnosis was sarcoma, presumptively liposarcoma, Oil Red O confirmed)
Individual cells vary from small and round or oval to large and polygonal with moderate amounts of eosinophilic, slightly granular cytoplasm. Many neoplastic cells also have one to multiple, clear, sharply demarcated vacuoles within the cyto- plasm. Nuclei vary from medium to large and round to oval to irregular and have coarsely stippled chromatin and small and often multiple nucle- oli. H&E,20 objective
What is your diagnosis? Lingual mass in a dog. Vet Clin Pathol 40/4 (2011) 561–562.
Tracings of whole blood platelet aggregation in a horse. Left is control. Describe and explain findings.
(Left) Healthy control horse; platelet aggregation occurred as expected up to 16 ohms. (Right) Peruvian Paso mare; platelet aggregation did not occur, indicating platelet dysfunction.
Glanzmann thrombasthenia in a 17-year-old Peruvian Paso mare. Vet Clin Pathol 40/1 (2011) 48–51
Fine-needle aspirate of a renal mass from a dog. Dx?
Dx: renal carcinoma
Large flat sheets of neoplastic tubular cells have basophilic and vacuolated cytoplasm, round to ovoid nuclei with reticular chromatin, and, occasionally, nucleoli. A large amount of necrotic tissue debris is also present. May Grunwald– Giemsa. (A) Bar = 50 mm. (B) Bar = 25 mm
Paraneoplastic leukocytosis in a dog with a renal carcinoma. Vet Clin Pathol 40/1 (2011) 89–94.
Cutaneous mass from a crevice kelpfish.
ID. Describe.
What is the stain in C?
ID the important structuers in D & E.
(A) Impression smear of a cutaneous mass from a crevice kelpfish. Note the monomorphic population of minimally pleomorphic round cells, 15–30 lmin diameter, with abundant round, reddish-brown, intracyoplasmic granules, approximately 1 lmdiameter. Modified Wright’s, bar = 20 lm. (B,C) Histologic sections of the mass. (B) Note sheets of round to polygonal cells with reddish-brown intracytoplasmic granules within scant fibrovascu- lar stroma. H&E, bar = 50 lm. (C) The cells strongly express melan A. Monoclonal anti-melanin A antibody. Fast Red (chromogen), bar = 50 lm. (D,E) Transmission electron micrographs of a neoplastic cell from the mass. Uranyl acetate and lead citrate. (D) The cell contains abundant pterinosomes. Bar = 1lm. (E) A pterinosome has a trilamellar membrane. Bar = 100 nm.
Chromatophoromain a crevice kelpfish (Gibbonsia montereyensis). Vet Clin Pathol 40/4 (2011) 549–552
Bone marrow aspirate from a dog. Dx? Describe the cells.
Dx: acute megakaryoblastic leukemia.
(A) A megakaryoblast with several cytoplasmic projections, (B) an atypical pro- megakaryocyte, (C) a dwarf megakaryocyte, and (D) a dysplastic megakaryocyte with dispersed nuclei, asynchronous nuclear to cytoplasmic maturation, and increased nuclear to cytoplasmic ratio are present. May-Grunwald Giemsa, 100 objective
Myeloperoxidase-positive acute megakaryoblastic leukemia in a dog. Vet Clin Pathol 40/4 (2011) 530–537
Bone marrow aspirate from a hyacinth macaw. Describe. Dx?
The specimen contained many cellular marrow particles. There were 33% large blasts with large central to eccentri- cally located round to oval nuclei, finely stippled chromatin, multiple prominent nucleoli, small amounts of deeply basophilic cytoplasm, and, occasionally, paranuclear Golgi zones. Moderate numbers of erythroid and myeloid precursors and a few small lymphocytes were present. Iron stores and thrombocytes appeared adequate.The initial interpretation was leukemia of unknown lineage.
What is your diagnosis? Bone marrow aspirate from a hyacinth macaw (Anodorhynchus hyacinthinus). Vet Clin Pathol 40/4 (2011) 565–566
Impression smears of the prescapular lymph node in a dog. Describe. What are the 2 stains used?
Irregularly branching hyphal structures often have a bulbous appearance. (A) Periodic acid-Schiff. (B) Gomori methenamine silver.
Granulomatous lymphadenitis caused by Talaromyces helicus in a Labrador Retriever. Vet Clin Pathol 40/4 (2011) 553–557.
Peripheral blood smear from a dog. Dx?
Bacteremia
What is your diagnosis? Blood smear from a dog. Vet Clin Pathol 40/2 (2011) 271–272.
Immunohistochemical staining of histologic sections of a renal carcinoma. What stian are the neoplastic cells positive for?
(A) Neoplastic epithelial cells strongly express GM-CSF in the cytoplasm. Bar = 20 mm. (B) Numerous neutrophils strongly express GM-CSF in the cytoplasm in a necrotic area of the tumor. Bar = 20 mm
Paraneoplastic leukocytosis in a dog with a renal carcinoma. Vet Clin Pathol 40/1 (2011) 89–94.
Fine-needle aspirate of a lung nodule from a dog. Modified-Wright’s stain. Dx?
Paragonimiasis
What is your diagnosis? Aspirate of a lung nodule in a dog. Vet Clin Pathol 40/1 (2011) 99–100.
Histologic section of a cutaneous mass in the mammary region. Dx?
Acantholytic squamous cell carcinoma (ASCC)
Note acantholytic cells in pseudoglandular lumina. H&E
What is your diagnosis? Cutaneous mass in the mammary region of a dog. Vet Clin Pathol 40/1 (2011) 101–102
Cutaneous mass in a dog. ID. What are special stains in the bottom 2 pictures?
(A) The dermis and subcutis are expanded by nodular accumulations of large round cells that efface normal architecture. Cells are arranged in sheets that are partially aligned along preexisting collagen fibers. H&E, bar = 200 mm. (B)The mass is composed of large round macrophages with abundant eosinophilic foamy cytoplasm. H&E, bar = 25 mm. (C) Lipid stained positively with Oil Red O is found in both the extracellular space andmacrophages. Oil Red O, bar = 25 mm. (D) The round cells have strong immunoreactivity for CD18 confirming they are of leukocyte origin. Mouse monoclonal antibody (University of California–Davis, diluted 1:20) and Nova Red chromagen, bar = 25 mm.
Idiopathic solitary cutaneous xanthoma in a dog. Vet Clin Pathol 40/1 (2011) 95–98
What is this cell?
Grey eosinophil in peripheral blood from an Italian Greyhound. Note the presence of clear vacuoles in the cytoplasm, representing unstained granules. May-Gru¨ nwald Giemsa,100 objective.
diameter
Grey eosinophils in sighthounds: frequency in 3 breeds and comparison of eosinophil counts determined manually and with 2 hematology analyzers. Vet Clin Pathol 40/4 (2011) 475–483.
Histologic section of bone marrow from a dog. Describe. Dx?
Dx: acute megakaryoblastic leukemia.
Note hypocellularity with 75% of marrow space occupied by adipose tissue. H&E, 94 objective. Inset: Nucleated cells are predominantly medium-sized and round with fewer large multinucleated cells (arrows). Small numbers of erythroid and myeloid precursors and normal mitotic figures (arrowhead) are occasionally seen. 40 objective
Myeloperoxidase-positive acute megakaryoblastic leukemia in a dog. Vet Clin Pathol 40/4 (2011) 530–537
Fine-needle aspirate of a cutaneous mass in the mammary region of an intact female mixed breed dog. Giemsa. Dx?
Acantholytic squamous cell carcinoma (ASCC)
What is your diagnosis? Cutaneous mass in the mammary region of a dog. Vet Clin Pathol 40/1 (2011) 101–102
Slide preparation of a fecal flotation (sugar solution) from a dog with lung nodules. Dx? Describe.
Paragonimiasis
An egg has an operculum (arrow) and a thickened ridge in the shell wall along the suture line of the operculum.
What is your diagnosis? Aspirate of a lung nodule in a dog. Vet Clin Pathol 40/1 (2011) 99–100
Lymphoplasmacytic cells in bone marrow of a dog with monoclonal IgM gammopathy. Dx?
Waldenstrom’s macroglobulinemia.
Waldenström’s macroglobulinemia in a dog with a bleeding diathesis. Vet Clin Pathol 40/3 (2011) 351–355
Impression smear of the prescapular lymph node of a dog. Describe.
(A) Irregularly septate hyphae have terminal pyriform asci. (B) Numerous fungal elements within a multinucleated giant cell.
Granulomatous lymphadenitis caused by Talaromyces helicus in a Labrador Retriever. Vet Clin Pathol 40/4 (2011) 553–557.
Peripheral blood smear from a dog. Dx? Decribe.
Acute megakaryoblastic leukemia. Note (A) blasts with cytoplasmic projections, cytoplasmic vacuolation, and eosinophilic granules (thick arrow) and with multiple cytoplasmic projections and adhered platelets (thin arrow) and (B) large blast that appears to be shedding platelets (arrow). Wright, 9100 objective.
Myeloperoxidase-positive acute megakaryoblastic leukemia in a dog. Vet Clin Pathol 40/4 (2011) 530–537
Fine-needle aspirate (A, B) and imprint (C) of a lingual mass in dog. Describe. Dx?
Liposarcoma (cyto dx was sarcoma, likely liposarc)
(A) Nucleated cells are arranged individually or in loose sheets or clusters among rare networks of capillaries and palisade-like arrangements. Variably sized clear vacuoles are noted in the background. 20 objective.
(B) Nucleated cells have indistinct cellular borders and pink finely granular cytoplasm; mild anisocytosis and an- isokaryosis are noted. Numerous small clear vacuoles are present in the background. 100 objective.
(C) Numerous clear vacuoles in the back- ground surround networks of capillaries and round to spindle-shaped cells. Some cells contain a single vacuole or multiple clear vacuoles and resemble lipoblasts. 20 objective.
Cytologic, histologic, and immunohistochemical features of lingual liposarcoma in a dog. Vet Clin Pathol 40/3 (2011) 393–397.
Impression smear of an ovarian mass in a mare. Giemsa. Dx?
Granulosa cell tumor
What is your diagnosis? Unilateral ovarian mass in a mare. Vet Clin Pathol 40/3 (2011) 399–400
Peripheral blood smear from a dog. Describe. Dx?
May–Hegglin anomaly
Note 2 giant platelets and neutrophils containing 1 or multiple cytoplasmic blue inclusions. Wright’s, 100 objective.
May–Hegglin anomaly in a dog. Vet Clin Pathol 40/2 (2011) 207–214.
Histologic section of a lymph node in a dog. Describe.
Multinucleate giant cells contain fungal hyphae with terminal asci. Periodic acid-Schiff, bar = 10 lm
Granulomatous lymphadenitis caused by Talaromyces helicus in a Labrador Retriever. Vet Clin Pathol 40/4 (2011) 553–557.
Bone marrow aspirate from a dog. Dx? Describe.
Dx: hronic monocytic leukemia. The sample is hypercellular and consists of a heterogeneous population of erythroid and myeloid precursors, including eosinophils, in various stages of maturation. Maturation is orderly. A possible monoblast is present (arrowhead). Wright-Giemsa, bar = 50 mm.
BCR-ABL translocation in a dog with chronic monocytic leukemia. Vet Clin Pathol 40/1 (2011) 40–47
Histologic section of spleen from a Labrador Retriever with acute megakaryoblastic leukemia. What are the 2 stains that these cells are positive for?
Neoplastic cells are immunoreactive for (A) CD61 and (B) myeloperoxidase.
Myeloperoxidase-positive acute megakaryoblastic leukemia in a dog. Vet Clin Pathol 40/4 (2011) 530–537
Imprint of a prescapular lymph node from a cow with severe anemia and leukopenia. Dx?
Bovine trypanosomiasis
What is your diagnosis? Lymphadenopathy in a cow with severe anemia. Vet Clin Pathol 40/1 (2011) 103–104.
Histologic section of a cerebral mass. Dx? Describe the stains used in B & C and the staining patterns.
Dx: Intracranial cavernous hemangioma
(A) H&E.
(B) factor VIII-RA; The cells lining vascular spaces as well as most mesenchymal cells separating vascular spaces demonstrated intense immunoreactivity for factor VIII-RA, supporting endothelial cell origin
(C) glial fibrillar acidic protein (GFAP). Rare intervening stromal cells expressed GFAP and represented entrapped glial cells within the mass.
What is your diagnosis? Intracranial mass in a dog. Vet Clin Pathol 40/4 (2011) 563–564.
Histologic section of a lingual mass in a dog. Describe. Dx?
Dx: Liposarcoma
(A) Well-differentiated portion of the tumor. Note cells with a single vacuole or multiple large clear vacuoles that displace the nucleus to the periphery of the cell.
(B) In some areas, the cells have eosinophilic granular cytoplasm without evidence of vacuolation.
Cytologic, histologic, and immunohistochemical features of lingual liposarcoma in a dog. Vet Clin Pathol 40/3 (2011) 393–397.
Fine-needle aspirate of a mass located in the perianal region in a cat. Dx?
Anal sac gland carcinoma
What is your diagnosis? Intracranial mass in a cat. Vet Clin Pathol 43/4 (2014) 611–612.
Cutaneous mass in a dog. ID.
Xanthoma
Idiopathic solitary cutaneous xanthoma in a dog. Vet Clin Pathol 40/1 (2011) 95–98
Blood film from a dog. Dx?
Chronic monocytic leukemia.
The cells have moderate amounts of lightly basophilic cytoplasm and pleomorphic nuclei with finely reticular chromatin. Infrequently, nucleoli were observed (2 cells in the center). Cells with prominent nucleoli such as these were included in the blast category. Wright-Giemsa, bar = 20 mm.
BCR-ABL translocation in a dog with chronic monocytic leukemia. Vet Clin Pathol 40/1 (2011) 40–47
