Vet Clin Path 2011 Flashcards
Typical flow cytometric results for erythrocytes from DEA 1.1-negative (A–C) and DEA 1.1-positive (D–F) dogs after incubating erythrocytes with anti-DEA 1.1 serum. Describe.
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Forward scatter (FSC-H) and side scatter (SSC-H) were used to gate erythrocytes and separate them from contaminating platelets (A, D).
In the DEA 1.1-positive samples (D), there was a characteristic second small population of gated cells with greater forward scatter, probably erythrocyte aggregates. Scatterplots of fluorescence intensity (FL1-H) vs forward scatter of 10,000 gated events (erythrocytes) showed increased fluorescence of DEA 1.1-positive erythrocytes with a characteristic reverse comma scatter (E). Frequency distribution plots (C, F) of fluorescence intensity showed a clear difference in fluorescence between DEA 1.1-negative (C) and DEA 1.1-positive (F) dogs. All scatter and fluorescence axes are log scales.
Flow cytometric assessment of canine erythrocytes and platelets for dog erythrocyte antigen 1.1. Vet Clin Pathol 40/4 (2011) 435–443
Fine-needle aspirate of an ulcerative skin lesion in a dog. Wright–Giemsa, 100 objective. Differentials?
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The differentials for the protozoal organisms included Neospora caninum, Toxoplasma gondii,and Sarcocystis spp.
Final dx was cutaneous neosporosis with suppurative inflammation.
What is your diagnosis? Fine-needle aspirate of ulcerative skin lesions in a dog. Vet Clin Pathol 40/3 (2011) 401–402
Imprint of a prescapular lymph node from a cow with severe anemia and leukopenia. Dx?
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Many trypomastigotes of Trypanosoma vivax are found among the RBCs and lymphocytes. Quick Romanowsky stain. Inset: Trypomastigote with centrally placed vesicular nucleus and sub- terminal kinetoplast.
What is your diagnosis? Lymphadenopathy in a cow with severe anemia. Vet Clin Pathol 40/1 (2011) 103–104.
Typical flow cytometric scatterplots for control platelets (A–D) and platelets from DEA 1.1-negative (E) and DEA 1.1-positive (F) dogs. Describe the results.
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Forward scatter (FSC-H) and side scatter (SSC-H) were used to gate washed platelets (A) and exclude microparticles and rare contaminating erythrocytes. Fluores- cence was recorded for 10,000 gated events (platelets). Platelet incubation with submaximal concentrations of positive control canine plasma contain- ing antibodies to platelet glycoprotein Ib/IX consistently generated strong fluorescence (FITC anti-IgG) for platelet surface-associated IgG (B). Incubation of platelets with buffer (C) consistently yielded negligible autofluorescence, and there was little increase in fluorescence when platelets were incubated with only the FITC-conjugated anti-[canine IgG] (D). There was no appreciable difference between the fluorescence of platelets from DEA 1.1-negative dogs (E) and DEA 1.1-positive dogs (F) after incubation with anti-DEA 1.1 serum.
Flow cytometric assessment of canine erythrocytes and platelets for dog erythrocyte antigen 1.1. Vet Clin Pathol 40/4 (2011) 435–443
Histologic section of the digestive system of a mussel. Dx? Describe the stages and structures in each image.
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Dx. Martiellosis (Marteilia refringens)
H&E.
(A) Early phase: M. refringens primary cells (a) in gut epithelial cells.
(B) Mature phase: sporangial structures with up to 8 internal spores (b); interstitial hemocytes, consistent with moderate inflammation; arrowhead, digestive cells with cytoplasmic vacuoles. Inset: Refringent bodies in the cytoplasm of a sporangium (arrow).
What is your diagnosis? Pale yellowish digestive gland and watery tissues in Mediterranean mussels. 274 Vet Clin Pathol 40/2 (2011) 273–274
Bone marrow aspirate from a hyacinth macaw. Describe. Dx?
Dx: leukemia of unknown lineage
Many blast cells and mitotic figures. Wright–Giemsa,100 objective.
What is your diagnosis? Bone marrow aspirate from a hyacinth macaw (Anodorhynchus hyacinthinus). Vet Clin Pathol 40/4 (2011) 565–566
Cytocentrifuged preparation of cerebrospinal fluid from a dog. Dx?
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Eosinophilic pleocytosis due to protothecosis
What is your diagnosis? Cerebrospinal fluid from a dog. Vet Clin Pathol 40/1 (2011) 105–106
Histologic section of a anal sac gland carcinoma located in the perianal region in a cat. Describe. Dx? What is the stain in the bottom picture?
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Anal sac carcioma
(A) Note the neoplastic cells arranged in papillae and palisades, the desmoplastic reaction, distinct single nucleoli, and a few mitotic figures. H&E, 9 20 objective.
(B) Note partial strong immuno- reactivity of neoplastic epithelial cells for CAM 5.2. H&E counterstain.
What is your diagnosis? Intracranial mass in a cat. Vet Clin Pathol 43/4 (2014) 611–612.
Fine-needle aspirate of a subconjunctival mass in the left eye of a horse. ID.
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Mycetoma caused by Scedosporium apiospermum
(A) Fungal hyphae with thick nonstaining cell walls (arrow). RBCs, cellular debris, degenerate neutrophils, and macrophages are present in the background. (B) Marked neutrophilic inflammation (arrow- head) and globular yeast-like fungal structures (arrow). Wright–Giemsa.
Subconjunctival mycetoma caused by Scedosporium apiospermum infection in a horse. Vet Clin Pathol 40/1 (2011) 84–88
Sedimented urine froman adultmale intact mixed-breed dog with hematuria. Dx?
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Dx: Dioctophyma renale infection
What is your diagnosis? Unexpected finding in sedimented urine from a dog. Vet Clin Pathol 40/3 (2011) 403–404.
Densitometer tracings obtained from agarose gel electrophoresis of (A) serum and (B) urine from a dog. Describe. Dx?
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Waldenstrom’s macroglobulinemia.
(A) Note the narrow peak in the b-globulin fraction, suggestive of the presence of a monoclonal paraprotein (albumin 35.1 g/ L, a1 4.7 g/L, a2 12.9 g/L, and b2 64.3 g/L). (B) Note the small peak in a similar region (total protein 6.5 g/L, albumin 2.3 g/L, b-fraction 0.9 g/L). Marked albuminuria is present, suggestive of glomerulopathy
Waldenström’s macroglobulinemia in a dog with a bleeding diathesis. Vet Clin Pathol 40/3 (2011) 351–355
Transmission electron micrograph of a neutrophil in the blood of a dog. Describe. Dx?
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May–Hegglin anomaly
Note the oval cytoplasmic inclusion (arrowheads) that is devoid of neutrophil granules and does not appear to be membrane-bound. Parallel thin filaments and ribosomes are present.
May–Hegglin anomaly in a dog. Vet Clin Pathol 40/2 (2011) 207–214.
Peritoneal fluid from a horse. Descibe. What is the material? What happened in this horse? What is the stain on the bottom?
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(A) Intracellular round, clear, nonrefractile globular material is present within neutrophils and macrophages. Diff-Quik. (B) The extracellular and intracellular glob- ular material stains pink to bright red. Oil Red O.
presumed leakage of mineral oil through a transmural rectal perforation
Oil Red O-positive lipid in peritoneal fluid from a horse with a rectal tear. Vet Clin Pathol 40/2 (2011) 265–269
Histologic section of brain in the region of the hippocampus of a dog. Describe. Dx? What is the stain the bottom image?
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(A) Note multifocal perivascular cuffs composed of aggregates of numerous lymphocytes, plasma cells, and small numbers of histiocytes. H&E. Inset: Inflammatory cells in a perivascular cuff. H&E, bar = 20 mm. (B) Note algal organisms with characteristic radially arranged multiple wedge-shaped endospores. Gomori’s methanamine silver
What is your diagnosis? Cerebrospinal fluid from a dog. Vet Clin Pathol 40/1 (2011) 105–106
Histologic section of a lingual liposarcoma in a dog. What 2 stains is this tumor positive for?
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Histologic section of a lingual liposarcoma in a dog.
A) S100 protein and (B) vimentin.
Cytologic, histologic, and immunohistochemical features of lingual liposarcoma in a dog. Vet Clin Pathol 40/3 (2011) 393–397.
Histologic section of spleen from a dog. Dx? Describe.
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Dx: acute megakaryoblastic leukemia. The red pulp is diffusely effaced by sheets of mononuclear round cells and low numbers of dysplastic megakaryocytes with dispersed nuclei (arrows). H&E, 920 objective; inset, 40 objective.
Myeloperoxidase-positive acute megakaryoblastic leukemia in a dog. Vet Clin Pathol 40/4 (2011) 530–537
Histologic section of a renal mass from a dog. Describe. Dx?
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Dx: renal carcinoma
(A) tubular to cribriform-like pattern, necrosis, and occasionally infiltrating neutrophils. (B) Neoplastic cells with variably vacuolated and eosinophilic cytoplasm, pleomorphic nuclei, and frequent mitotic figures
Paraneoplastic leukocytosis in a dog with a renal carcinoma. Vet Clin Pathol 40/1 (2011) 89–94.
Impression smear of lingual mass in a dog. Dx?
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Liposarcoma (The cytologic diagnosis was sarcoma, and differential diagnoses included fibrosarcoma, liposarcoma, hemangiosarcoma, rhabdomyosarcoma, melanoma, or other soft tissue sarcoma)
What is your diagnosis? Lingual mass in a dog. Vet Clin Pathol 40/4 (2011) 561–562.
Clot retraction in blood samples from a Peruvian Paso mare and a healthy control horse. Describe the results.
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Clot retraction was normal in the control sample, but no retraction was evident in the sample from the mare after several hours, supporting abnormal platelet function.
Glanzmann thrombasthenia in a 17-year-old Peruvian Paso mare. Vet Clin Pathol 40/1 (2011) 48–51
Blood smear from hyacinth macaw. Describe.
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A few folded and dacryocyte-like erythrocytes. Wright–Giemsa, 100 objective.
What is your diagnosis? Bone marrow aspirate from a hyacinth macaw (Anodorhynchus hyacinthinus). Vet Clin Pathol 40/4 (2011) 565–566
Histology of an ovarian mass in a mare. Dx? Describe structures in the inset.
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Dx: granulosa-theca cell tumor
Note aggregates of neoplastic granulosa cells surrounded by eosinophilic stroma. H&E. Inset: Call-Exner body surrounded by neoplastic granulosa cells. 40 objective.
What is your diagnosis? Unilateral ovarian mass in a mare. Vet Clin Pathol 40/3 (2011) 399–400
Impression smear of an ovarian mass in a mare. Dx?
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Dx: granulosa-theca cell tumor
A longitudinal groove, with dense chromatin in the shape of an ‘‘H,’’ is present in a nucleus (arrow). Giemsa
What is your diagnosis? Unilateral ovarian mass in a mare. Vet Clin Pathol 40/3 (2011) 399–400
ID the structures
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Blood film from a dog with Babesia canis rossi infection. Protozoal piroplasms within RBCs vary from round (A) to piriform (B) and are sometimes present in large numbers (C). An occasional ghost RBC (D, arrows) contains a piroplasm. Protozoal merozoites are also present extracellularly (E, F). Aqueous Romanowsky stain, bar = 10 mm.
Babesia canis rossi infection in a Texas dog; Vet Clin Pathol 40/3 (2011) 345–350