EM Flashcards
What are these cells? Describe them.
Thrombocytes in the Cape vulture.
(a) A discoid thrombocyte with a smooth surface and another with pseudopodia which indicates activation (arrow). (b) Two thrombocytes with pseudopodia. Note the crater-like depression (arrow) in one cell.
“Thrombocyte morphology and morphometric observations in two vulture species” Vet Clin Pathol 38/3 (2009) 316–320
What do the images represent? Describe the pathologic changes observed.
The left-hand panel in part (B) illustrates extensive cytoplasmic vacuolation (black arrow) and mitochondrial swelling and cristeolysis (white arrow), whereas the right-hand panel shows increased intermicrovillar spacing (black arrow), in an endoscopic biopsy harvested from a dog with chronic enteropathy before dietary treatment
“A Comprehensive Pathological Survey of Duodenal Biopsies from Dogs with Diet-Responsive Chronic Enteropathy” J Vet Intern Med 2013;27:862–874
What does the image represent? What are the arrows pointing to?
Numerous immune complexes on the abluminal surface of the glomerular basement membrane
“Prevalence of Immune-Complex Glomerulonephritides in Dogs
Biopsied for Suspected Glomerular Disease: 501 Cases (2007–2012)” J Vet Intern Med 2013;27:S67–S75
What do the images represent? What are the arrows?What are the asterisks?
Embryonal rhabdomyosarcoma. (A) Note pleomorphic neoplastic cells with prominent nucleoli and intracytoplasmic coarse dense masses (arrow), paral- leling the cytoplasmic expression of desmin. (B) Note the intracytoplasmic masses of tangled myofilaments (asterisks) in a neoplastic cell.
“Subcutaneous embryonal rhabdomyosarcoma in a dog: cytologic, immunocytochemical, histologic, and ultrastructural features” Vet Clin Pathol 39/4 (2010) 499–504
What are the structures in the images? What species are these found in? What do the arrows and astericks represent?
Transmission electron micrograph of a typical vulture (Cape vulture) thrombocyte.
(a) Cross-sectioned microtubules (thick arrow), mitochondria (thin arrow), and a dense body.
(b) A large lipid droplet (*).
(c) The Golgi complex is just above the centriole (arrow).
(b and d) Opening of the surface connecting canalicular system with a depression on the surface (arrow).
“Thrombocyte morphology and morphometric observations in two vulture species” Vet Clin Pathol 38/3 (2009) 316–320
Describe the cell in the picuture. What is the conditon?
Transmission electron micrograph of oviduct cilia from a dog with primary ciliary dyskinesia
In normal cilia, there is a 9+2 arrangement of microtubules with 2 single microtubules in the central position surrounded by a peripheral ring composed of 9 pairs of microtu- bules. The 3 cilia (1, 2, and 3) present in this preparation are ab- normal. Notice that there are only 8 peripheral microtubules, and some of these are singlets rather than doublets (white arrows). Additionally, the 2 microtubules that are normally in the central position are often absent and instead replaced by a peripheral doublet microtubule (black arrow). Both inner and outer dynein arms are missing from the peripheral microtubules.
PIP JAVMA, Vol 244, No. 4, February 15, 2014
What cell is this? Describe it and what the arrow is pointing to.
One giant platelet with many dense granules, glycogen granules, ribosomes, and some a-granules (arrow).
“Characterization of blood cells in the Leopard Cat” Vet Clin Pathol 39/2 (2010) 193–198
Describe the cell in the picture. What is the arrow pointing at? What is the condition and pathophys?
Transmission electron micrograph of a neutrophil in the blood of a dog with May-Hegglin anomaly. Note the oval cytoplasmic inclusion (arrowheads) that is devoid of neutrophil granules and does not appear to be membrane-bound. Parallel thin filaments and ribosomes are present.
MHA, a hereditary autosomal dominant disorder reported in people, is associated with mutation of MYH9, a 40-exon gene encoding NMM-IIA.1,2 MHA results from mutations in exons encoding the heavy chains (NMMHC-IIA) of the protein.
“May–Hegglin anomaly in a dog” Vet Clin Pathol 40/2 (2011) 207–214
What are the structures in the images? What species are these found in?
Transmission electron micrographs of a neoplastic cell from a cutaneous chromatophoroma (neoplasia of pigment-containing and light-reflecting cells in cold-blooded animals) in a crevice kelpfish.
(D) The cell contains abundant pterinosomes (pigment- containing organelles found in normal erythrophores and xanthophores)
(E) A pterinosome has a trilamellar membrane
“Chromatophoromain a crevice kelpfish (Gibbonsia montereyensis)” Vet Clin Pathol 40/4 (2011) 549–552
What do these images represent? How is D different?
Heinz bodies in a Red Panda (A,E), a dog (B,D,F) and a cat (C). These RBCs were exposed to skunk musk.
D is a ghost RBC with Heinz bodies.
“Skunk musk causesmethemoglobin and Heinz body formation in vitro” Vet Clin Pathol 42/3 (2013) 291–300
What does the image represent?
Manatee heterophil containing many variably sized and variably shaped granules. A few large, more densely stained granules may be primary granules, with smaller less densely stained granules being secondary granules.
“Hematology of healthy Florida manatees” Vet Clin Pathol 38/2 (2009) 183–193
What structures do the images represent? What are the arrows pointing to in each picture and what is the specific location?
A. Immune complexes (white arrows) in the abluminal side (subepithelial) of the renal capillary wall.
B. Immune complexes (white arrows) along the luminal side (subendothelial) of the renal capillary wall.
“Pathologic Evaluation of Canine Renal Biopsies: Methods for Identifying Features that Differentiate Immune-Mediated Glomerulonephritides from Other Categories of Glomerular Diseases” J Vet Intern Med 2013;27:S10–S18
What is the image of?
(H) Basophil with large vacuoles; some vacuoles are filled with dense granules. (I) Basophil with fine lamellar and electron-dense granules.
“Hematology and clinical chemistry of adult yellow-headed temple turtles in Thailand”
What does the image represent? What are the star and arrow denoting?
Splenic macrophage from a severe combined immunodeficiency foal showing phagocytized Theileria equi-infected erythrocytes. Arrow denotes the organism inside an erythrocyte and the asterisk illustrates the nucleus of the splenic macrophage.
“Review of Equine Piroplasmosis” J Vet Intern Med 2013;27:1334–1346
What does the image represent?
Nodule-bearing section of liver from a screech owl found dead. In this image, a neoplastic myelocyte with heterogeneous granules of variable size (maximum dimension, 0.25 to 1.9 µm) is visible. The granules are spherical with moderate to high electron density. The nucleus has a prominent, electron-dense, granular nucleolus and clumped chromatin. No viral particles are evident.
Diagnosis was hepatic and splenic myelocytomatosis
“Pathology in Practice” JAVMA, Vol 244, No. 12, June 15, 2014
What is this structure? Describe it and the internal structures. What is its clinical significance?
Transmission electron micrograph of a Blastocystis protozoal cyst in feces from a dog.
The cyst is ovoid and contains a prominent, electron-lucent vacuole (V) filled with coarse, electron-dense material. The cytoplasm (C) also is filled with coarse, electron-dense material persumed to be ribosomes and polyribosomes. A single, round nucleus (N) is composed predominantly of euchromatin with a small ring of heterochromatin.
Considered clinically insignificant in this dog.
“What is your diagnosis? Rectal scraping from a dog with diarrhea” Vet Clin Pathol 38/1 (2009) 59–62
What does the image represent? What are the arrowheads pointing to?
Widespread effacement of podocyte foot processes (arrowheads) and absence of immune complexes
“Prevalence of Immune-Complex Glomerulonephritides in Dogs
Biopsied for Suspected Glomerular Disease: 501 Cases (2007–2012)” J Vet Intern Med 2013;27:S67–S75
What does the structure in the image represent? What is the astricks pointint at?
Amyloidois in a renal biopsy from a dog. Expansion of the mesangial zone by haphazardly arranged fibrils. Inset: Higher magnification of fibrils.
“Pathologic Evaluation of Canine Renal Biopsies: Methods for Identifying Features that Differentiate Immune-Mediated Glomerulonephritides from Other Categories of Glomerular Diseases” J Vet Intern Med 2013;27:S10–S18
What is the image of?
(D) Heterophil. (E) Granules of heterophil with distinct central cores.
“Hematology and clinical chemistry of adult yellow-headed temple turtles in Thailand”
What cell is this?
Basophil in a leopard.
“Characterization of blood cells in the Leopard Cat” Vet Clin Pathol 39/2 (2010) 193–198
What structures does the images represent? What are the dotted arrows pointing to?
Non-immune complex glomerulonephritis. Thickened glomerular basement membrane with multifocal electron-dense material (dotted arrows) that is consistent with lipid (E) and hyalinosis (F). Immune complexes weren’t identified.
“Pathologic Evaluation of Canine Renal Biopsies: Methods for Identifying Features that Differentiate Immune-Mediated Glomerulonephritides from Other Categories of Glomerular Diseases” J Vet Intern Med 2013;27:S10–S18
What does the image represent?
Manatee eosinophil containing large, round, densely stained granules.
“Hematology of healthy Florida manatees” Vet Clin Pathol 38/2 (2009) 183–193
What are the cells in the image? Describe them. What are the arrows pointing to?
Ultrathin section of a large Heinz body (arrow) within an RBC ghost. The Heinz body is electron-dense, and dense aggregates of reticular material of similar electron density are associated with the plasma membrane; 3 platelets are evident.
“Copper toxicosis in a Boer goat” Vet Clin Pathol 41/4 (2012) 502–508
What is the image of?
(F) Eosinophils with homogenously electron-dense granules
“Hematology and clinical chemistry of adult yellow-headed temple turtles in Thailand”
What kind of cell is this?
Eosinophils in a leopard
“Characterization of blood cells in the Leopard Cat” Vet Clin Pathol 39/2 (2010) 193–198
What is the image of?
Lymphocyte
“Hematology and clinical chemistry of adult yellow-headed temple turtles in Thailand”
What is the image of?
Azurophil
“Hematology and clinical chemistry of adult yellow-headed temple turtles in Thailand”
What does the structure represent? What species is this in? What are the arrows pointing at?
Transmission electron photomicrograph of a typical vulture thrombocyte (Cape vulture). A dense body with an electron dense gran- ule (long arrow) and a lipid droplet can be seen toward the right pole of the cell. An apparent empty vacuole (short arrow) and centriole can be seen in the left pole.
“Thrombocyte morphology and morphometric observations in two vulture species” Vet Clin Pathol 38/3 (2009) 316–320
What does the image represent?
Peripheral blood from a dog with elliptocytosis caused by a mutant b-spectrin.
“Canine elliptocytosis due to a mutant b-spectrin” Vet Clin Pathol 38/1 (2009) 52–58
What are these cells and what are the arrows pointing to?
Red blood cells in a leopard. Arrows are Heinz bodies.
“Characterization of blood cells in the Leopard Cat” Vet Clin Pathol 39/2 (2010) 193–198
