vaccinatie

Question 1

alternatief levende vaccins

Answer 1

• dode viruselementen
• subunit vaccins (antigen)
• DNA vaccins (plasmide)
• mRNA vaccins

Question 2

levende vaccins

Answer 2

• verzwakte vorm -> minder virulentie
  -> subcultuur in suboptimale condities
  -> mutagenese virulentiegenen
  -> mutagenese basis biochemische pathways

Question 3

recombinante vorm levende vaccins

Answer 3

• gen virus A -> levend virus X of ander immunogeen org
• gen virus A, gen virus B,… -> levend virus X
  => via in vivo recombinatie in E.coli

virus X kandidaten
- vaccina
- verzwakt salmonella (geen virus)
- tubercolosis BCG (geen virus)

gen virus A
- plasmide (geen AB resistentieselectie!)
- integratievector
- suicide vector (wegdiuleren)